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Modern approaches for identification of modified nucleotides in RNA. / Filippova, J. A.; Semenov, D. V.; Juravlev, E. S. et al.

In: Biochemistry (Moscow), Vol. 82, No. 11, 01.11.2017, p. 1217-1233.

Research output: Contribution to journal › Review article › peer-review

Harvard

Filippova, JA, Semenov, DV, Juravlev, ES, Komissarov, AB, Richter, VA & Stepanov, GA 2017, 'Modern approaches for identification of modified nucleotides in RNA', Biochemistry (Moscow), vol. 82, no. 11, pp. 1217-1233. https://doi.org/10.1134/S0006297917110013

APA

Filippova, J. A., Semenov, D. V., Juravlev, E. S., Komissarov, A. B., Richter, V. A., & Stepanov, G. A. (2017). Modern approaches for identification of modified nucleotides in RNA. Biochemistry (Moscow), 82(11), 1217-1233. https://doi.org/10.1134/S0006297917110013

Vancouver

Filippova JA, Semenov DV, Juravlev ES, Komissarov AB, Richter VA, Stepanov GA. Modern approaches for identification of modified nucleotides in RNA. Biochemistry (Moscow). 2017 Nov 1;82(11):1217-1233. doi: 10.1134/S0006297917110013

Author

Filippova, J. A. ; Semenov, D. V. ; Juravlev, E. S. et al. / Modern approaches for identification of modified nucleotides in RNA. In: Biochemistry (Moscow). 2017 ; Vol. 82, No. 11. pp. 1217-1233.

BibTeX

@article{cb13732a46244f159c0317269d4c824b,
title = "Modern approaches for identification of modified nucleotides in RNA",
abstract = "This review considers approaches for detection of modified monomers in the RNA structure of living organisms. Recently, some data on dynamic alterations in the pool of modifications of the key RNA species that depend on external factors affecting the cells and physiological conditions of the whole organism have been accumulated. The recent studies have presented experimental data on relationship between the mechanisms of formation of modified/minor nucleotides of RNA in mammalian cells and the development of various pathologies. The development of novel methods for detection of chemical modifications of RNA nucleotides in the cells of living organisms and accumulation of knowledge on the contribution of modified monomers to metabolism and functioning of individual RNA species establish the basis for creation of novel diagnostic and therapeutic approaches. This review includes a short description of routine methods for determination of modified nucleotides in RNA and considers in detail modern approaches that enable not only detection but also quantitative assessment of the modification level of various nucleotides in individual RNA species.",
keywords = "chemical modifications of nucleotides, mass spectrometry, minor nucleotides, post-transcriptional RNA modifications, reverse transcription, RNA editing, transcriptome-wide high-throughput sequencing",
author = "Filippova, {J. A.} and Semenov, {D. V.} and Juravlev, {E. S.} and Komissarov, {A. B.} and Richter, {V. A.} and Stepanov, {G. A.}",
year = "2017",
month = nov,
day = "1",
doi = "10.1134/S0006297917110013",
language = "English",
volume = "82",
pages = "1217--1233",
journal = "Biochemistry (Moscow)",
issn = "0006-2979",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "11",

}

RIS

TY - JOUR

T1 - Modern approaches for identification of modified nucleotides in RNA

AU - Filippova, J. A.

AU - Semenov, D. V.

AU - Juravlev, E. S.

AU - Komissarov, A. B.

AU - Richter, V. A.

AU - Stepanov, G. A.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - This review considers approaches for detection of modified monomers in the RNA structure of living organisms. Recently, some data on dynamic alterations in the pool of modifications of the key RNA species that depend on external factors affecting the cells and physiological conditions of the whole organism have been accumulated. The recent studies have presented experimental data on relationship between the mechanisms of formation of modified/minor nucleotides of RNA in mammalian cells and the development of various pathologies. The development of novel methods for detection of chemical modifications of RNA nucleotides in the cells of living organisms and accumulation of knowledge on the contribution of modified monomers to metabolism and functioning of individual RNA species establish the basis for creation of novel diagnostic and therapeutic approaches. This review includes a short description of routine methods for determination of modified nucleotides in RNA and considers in detail modern approaches that enable not only detection but also quantitative assessment of the modification level of various nucleotides in individual RNA species.

AB - This review considers approaches for detection of modified monomers in the RNA structure of living organisms. Recently, some data on dynamic alterations in the pool of modifications of the key RNA species that depend on external factors affecting the cells and physiological conditions of the whole organism have been accumulated. The recent studies have presented experimental data on relationship between the mechanisms of formation of modified/minor nucleotides of RNA in mammalian cells and the development of various pathologies. The development of novel methods for detection of chemical modifications of RNA nucleotides in the cells of living organisms and accumulation of knowledge on the contribution of modified monomers to metabolism and functioning of individual RNA species establish the basis for creation of novel diagnostic and therapeutic approaches. This review includes a short description of routine methods for determination of modified nucleotides in RNA and considers in detail modern approaches that enable not only detection but also quantitative assessment of the modification level of various nucleotides in individual RNA species.

KW - chemical modifications of nucleotides

KW - mass spectrometry

KW - minor nucleotides

KW - post-transcriptional RNA modifications

KW - reverse transcription

KW - RNA editing

KW - transcriptome-wide high-throughput sequencing

UR - http://www.scopus.com/inward/record.url?scp=85034254538&partnerID=8YFLogxK

U2 - 10.1134/S0006297917110013

DO - 10.1134/S0006297917110013

M3 - Review article

C2 - 29223150

AN - SCOPUS:85034254538

VL - 82

SP - 1217

EP - 1233

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - 11

ER -

ID: 9675298