Research output: Contribution to journal › Article › peer-review
Microvasculature in hepatocellular carcinoma : An ultrastructural study. / Taskaeva, Iuliia; Bgatova, Nataliya.
In: Microvascular Research, Vol. 133, 104094, 01.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Microvasculature in hepatocellular carcinoma
T2 - An ultrastructural study
AU - Taskaeva, Iuliia
AU - Bgatova, Nataliya
N1 - Funding Information: This work was supported with financing of the Novosibirsk Research Institute of Clinical and Experimental Lymphology as part of a state order, no. 0324-2019-0045. Publisher Copyright: © 2020 Elsevier Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Background: Hepatocellular carcinoma (HCC) is one of the most vascularized tumor types, and is characterized by development of heterogeneous immature vessels with increased permeability. Here, we analyzed morphology and vascular permeability-related structures in endothelial cells of HCC microvessels. Methods: Small (Type I) and large (Type II) peritumoral blood microvessels were assessed in HCC-bearing mice. By transmission electron microscopy, endothelial cell cytoplasm area, free transport vesicles, vesiculo-vacuolar organelles and clathrin-coated vesicles were measured. Results: The phenotypic changes in the HCC microvessels included presence of sinusoidal capillarization, numerous luminal microprocesses and abnormal luminal channels, irregular dilatations of interendothelial junctions, local detachment of basement membranes and widened extracellular space. Endothelial cells Type I microvessels showed increased vesicular trafficking-related structures. Conclusion: Ultrastructural characteristics of microvessels Type I can associate with HCC new-formed microvessels. The morphological changes observed in HCC microvessels might explain the increased transcellular and paracellular permeability in HCC endothelial cells.
AB - Background: Hepatocellular carcinoma (HCC) is one of the most vascularized tumor types, and is characterized by development of heterogeneous immature vessels with increased permeability. Here, we analyzed morphology and vascular permeability-related structures in endothelial cells of HCC microvessels. Methods: Small (Type I) and large (Type II) peritumoral blood microvessels were assessed in HCC-bearing mice. By transmission electron microscopy, endothelial cell cytoplasm area, free transport vesicles, vesiculo-vacuolar organelles and clathrin-coated vesicles were measured. Results: The phenotypic changes in the HCC microvessels included presence of sinusoidal capillarization, numerous luminal microprocesses and abnormal luminal channels, irregular dilatations of interendothelial junctions, local detachment of basement membranes and widened extracellular space. Endothelial cells Type I microvessels showed increased vesicular trafficking-related structures. Conclusion: Ultrastructural characteristics of microvessels Type I can associate with HCC new-formed microvessels. The morphological changes observed in HCC microvessels might explain the increased transcellular and paracellular permeability in HCC endothelial cells.
KW - Blood capillaries
KW - Endothelial cell
KW - Hepatocellular carcinoma
KW - Microvasculature
KW - Morphology
KW - Ultrastructure
KW - Vascular permeability
KW - Vesicular trafficking
UR - http://www.scopus.com/inward/record.url?scp=85092241061&partnerID=8YFLogxK
U2 - 10.1016/j.mvr.2020.104094
DO - 10.1016/j.mvr.2020.104094
M3 - Article
C2 - 33011171
AN - SCOPUS:85092241061
VL - 133
JO - Microvascular Research
JF - Microvascular Research
SN - 0026-2862
M1 - 104094
ER -
ID: 27374419