TY - JOUR

T1 - Microstructure of lymphoid tissue in lymph nodes of different localization in young, mature and old rats

AU - Demchenko, Georgii A.

AU - Abdreshov, Serik N.

AU - Yessimsiitova, Zura

AU - Gorchakov, Vladimir

AU - Berdikulov, Maxat

AU - Khussainov, Damir

AU - Koibasova, Laura

AU - Lessova, Zhanikha

N1 - Demchenko, GA, Abdreshov, SN, Yessimsiitova, Z, Gorchakov, V, Berdikulov, M, Khussainov, D, Koibasova, L, Lessova, Z 2025, Microstructure of lymphoid tissue in lymph nodes of different localization in young, mature and old rats. Caspian Journal of Environmental Sciences, 23: 979-990. This work was supported by the Program-targeted funding for scientific, scientific-technical programs BR 24993032 Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan.

PY - 2025/10

Y1 - 2025/10

N2 - Aging is a natural biological process of gradual degradation of parts and systems of the human body and the consequences of this process. These changes occur in the human genotype, both under the influence of environmental factors and as a result of endogenous processes, namely, a deficiency of antioxidant enzymes, changes in the immune system and the development of chronic inflammation. The lymphatic system is the internal environment of the body. In recent years, we and other scientists have determined general and regional signs of aging of the lymphatic system, reflecting a decrease in drainage-detoxification and immune functions, caused by a deficiency of trace elements in the lymph nodes. Considering the important role of the lymphatic system in the body, its participation in tissue drainage and its compensatory and adaptive functions, it is of interest to develop a program of restorative methods of lymphatic system functions on the processes occurring in the aging organism, and thereby contribute to the prolongation of life. This study aimed to determine the ratio of functional zones and microstructures of lymphoid tissue in nodes of different locations in young, mature, and senile rats. Lymph nodes were analyzed using Avtandilov's morphometry, and the samples were fixed in 10% neutral buffered solution formalin or Telesnitsky's fluid, processed, and embedded in paraffin for histological sectioning with various dyes, including hematoxylin and eosin. This study presents data on aging affects the lymph node microstructure and functional area. In young and mature animals, no significant differences were observed. However, aging leads to thickening of the capsule and increased connective tissue around blood vessels, disrupting the lymphoid lobule structure and causing lymph drainage issues. In older animals, the medulla remains unchanged as long as lymph flow is maintained. Compared to younger animals, older ones showed more connective tissue and fewer lymphoid nodules. There is a decreased in certain immune cell types, indicating lower immunoreactivity. Conclusion: In young animals, no significant changes were observed compared to mature animals. Ageing in lymph nodes showed increased connective tissue and reduced lymphoid nodules, with a decrease in certain cell types, and an elevation in the number of reticular cells, indicating lower immunoreactivity.

AB - Aging is a natural biological process of gradual degradation of parts and systems of the human body and the consequences of this process. These changes occur in the human genotype, both under the influence of environmental factors and as a result of endogenous processes, namely, a deficiency of antioxidant enzymes, changes in the immune system and the development of chronic inflammation. The lymphatic system is the internal environment of the body. In recent years, we and other scientists have determined general and regional signs of aging of the lymphatic system, reflecting a decrease in drainage-detoxification and immune functions, caused by a deficiency of trace elements in the lymph nodes. Considering the important role of the lymphatic system in the body, its participation in tissue drainage and its compensatory and adaptive functions, it is of interest to develop a program of restorative methods of lymphatic system functions on the processes occurring in the aging organism, and thereby contribute to the prolongation of life. This study aimed to determine the ratio of functional zones and microstructures of lymphoid tissue in nodes of different locations in young, mature, and senile rats. Lymph nodes were analyzed using Avtandilov's morphometry, and the samples were fixed in 10% neutral buffered solution formalin or Telesnitsky's fluid, processed, and embedded in paraffin for histological sectioning with various dyes, including hematoxylin and eosin. This study presents data on aging affects the lymph node microstructure and functional area. In young and mature animals, no significant differences were observed. However, aging leads to thickening of the capsule and increased connective tissue around blood vessels, disrupting the lymphoid lobule structure and causing lymph drainage issues. In older animals, the medulla remains unchanged as long as lymph flow is maintained. Compared to younger animals, older ones showed more connective tissue and fewer lymphoid nodules. There is a decreased in certain immune cell types, indicating lower immunoreactivity. Conclusion: In young animals, no significant changes were observed compared to mature animals. Ageing in lymph nodes showed increased connective tissue and reduced lymphoid nodules, with a decrease in certain cell types, and an elevation in the number of reticular cells, indicating lower immunoreactivity.

KW - Aging

KW - Cells

KW - Lymphatic system

KW - Lymphoid tissue

KW - Microstructure

UR - https://www.scopus.com/pages/publications/105024537848

UR - https://www.mendeley.com/catalogue/4fdddf9e-51bb-35f3-b9c6-338a01f3d878/

U2 - 10.22124/cjes.2025.9213

DO - 10.22124/cjes.2025.9213

M3 - Article

VL - 23

SP - 979

EP - 990

JO - Caspian Journal of Environmental Sciences

JF - Caspian Journal of Environmental Sciences

SN - 1735-3033

IS - 4

M1 - 13

ER -