TY - JOUR

T1 - Method for correction of experimental action spectrum using actual overlapping spectra of radiation sources

AU - Lyulyukin, Mikhail

AU - Kovalevskiy, Nikita

AU - Timofeeva, Sofya

AU - Gusachenko, Egor

AU - Solovyeva, Maria

AU - Selishchev, Dmitry

AU - Kozlov, Denis

N1 - Funding Information: The reported study was funded by RFBR, project number 19-33-60019. The research was performed using equipment of the Shared-Use Center "National Center for the Study of Catalysts" at the Boreskov Institute of Catalysis Siberian Branch of the Russian Academy of Sciences. © 2021 The Authors. Published by Elsevier B.V.

PY - 2021/1

Y1 - 2021/1

N2 - Experimental dependency of the photosystem's response on the wavelength of exciting radiation, also known as action spectrum, may be substantially affected by the spectrum shape of this radiation. This is especially important in the case, when different radiation sources are used for the investigation of action spectrum. For instance, too wide emission peaks of radiation sources can blur the scopes of actual action spectrum and distort information about the properties of photosystem at certain wavelength regions. Here, we propose a method for the correction of experimental action spectrum by the recalculation of experimental data of photoresponse according to actual spectra of exciting radiation. In the case of overlapping radiation spectra from different radiation sources, this method results in much better correlation of experimental action spectrum to actual action spectrum or absorption spectrum of photosystem. The data on photoactivity of several photocatalysts are presented to illustrate and validate the proposed method. • Activity of photosystem depends on the actual spectrum of the radiation source • Single-peak optical radiation sources with the same basic wavelength may cause a different photoactivity • Effect of actual spectrum of the light source on the photoactivity is to be considered

AB - Experimental dependency of the photosystem's response on the wavelength of exciting radiation, also known as action spectrum, may be substantially affected by the spectrum shape of this radiation. This is especially important in the case, when different radiation sources are used for the investigation of action spectrum. For instance, too wide emission peaks of radiation sources can blur the scopes of actual action spectrum and distort information about the properties of photosystem at certain wavelength regions. Here, we propose a method for the correction of experimental action spectrum by the recalculation of experimental data of photoresponse according to actual spectra of exciting radiation. In the case of overlapping radiation spectra from different radiation sources, this method results in much better correlation of experimental action spectrum to actual action spectrum or absorption spectrum of photosystem. The data on photoactivity of several photocatalysts are presented to illustrate and validate the proposed method. • Activity of photosystem depends on the actual spectrum of the radiation source • Single-peak optical radiation sources with the same basic wavelength may cause a different photoactivity • Effect of actual spectrum of the light source on the photoactivity is to be considered

KW - Action spectra

KW - Basic wavelength

KW - Irradiation

KW - Method for correction of action spectrum using irradiation spectra (MCASIS)

KW - Photoresponse

KW - Spectra

UR - http://www.scopus.com/inward/record.url?scp=85099356177&partnerID=8YFLogxK

U2 - 10.1016/j.mex.2021.101221

DO - 10.1016/j.mex.2021.101221

M3 - Article

C2 - 34434744

AN - SCOPUS:85099356177

VL - 8

JO - MethodsX

JF - MethodsX

SN - 2215-0161

M1 - 101221

ER -