Standard

Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation. / Butikova, Ekaterina A.; Basov, Nikita V.; Rogachev, Artem D. et al.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1870, No. 2, 159595, 03.2025.

Research output: Contribution to journal › Article › peer-review

Harvard

Butikova, EA, Basov, NV, Rogachev, AD, Gaisler, EV, Ivanisenko, VA, Demenkov, PS, Makarova, ALA, Ivanisenko, TV, Razumov, IA, Kolomeyets, DA, Cheresiz, SV, Solovieva, OI, Larionov, KP, Sotnikova, YS, Patrushev, YV, Kolchanov, NA, Pokrovsky, AG, Vinokurov, NA, Kanygin, VV, Popik, VM & Shevchenko, OA 2025, 'Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, vol. 1870, no. 2, 159595. https://doi.org/10.1016/j.bbalip.2025.159595

APA

Butikova, E. A., Basov, N. V., Rogachev, A. D., Gaisler, E. V., Ivanisenko, V. A., Demenkov, P. S., Makarova, A. L. A., Ivanisenko, T. V., Razumov, I. A., Kolomeyets, D. A., Cheresiz, S. V., Solovieva, O. I., Larionov, K. P., Sotnikova, Y. S., Patrushev, Y. V., Kolchanov, N. A., Pokrovsky, A. G., Vinokurov, N. A., Kanygin, V. V., ... Shevchenko, O. A. (2025). Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1870(2), [159595]. https://doi.org/10.1016/j.bbalip.2025.159595

Vancouver

Butikova EA, Basov NV, Rogachev AD, Gaisler EV, Ivanisenko VA, Demenkov PS et al. Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2025 Mar;1870(2):159595. doi: 10.1016/j.bbalip.2025.159595

Author

Butikova, Ekaterina A. ; Basov, Nikita V. ; Rogachev, Artem D. et al. / Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation. In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2025 ; Vol. 1870, No. 2.

BibTeX

@article{7bb62dc12f0f49e6b5038ec42887e168,
title = "Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation",
abstract = "Terahertz (THz) radiation has gained attention due to technological advancements, but its biological effects remain unclear. We investigated the impact of 2.3 THz radiation on SK-MEL-28 cells using metabolomic and gene network analysis. Forty metabolites, primarily related to purine, pyrimidine synthesis and breakdown pathways, were significantly altered post-irradiation. Lipids, such as ceramides and phosphatidylcholines, were also affected. Gene network reconstruction and analysis identified key regulators of the enzymes involved in biosynthesis and degradation of significantly altered metabolites. Mitochondrial membrane components, such as the respiratory chain complex, the proton-transporting ATP synthase complex, and components of lipid rafts reacted to THz radiation. We propose that THz radiation induces reversible disruption of the lipid raft macromolecular structure, thereby altering mitochondrial molecule transport while maintaining protein integrity, which explains the high cell survival rate. Our findings enhance the understanding of THz biological effects and emphasize the role of membrane components in the cellular response to THz radiation.",
keywords = "ANDSystem, Biological effects of non-ionizing radiation, Biophotonics, Cell metabolomics, Infrared radiation, Lipid rafts, Melanoma, THz, Targeted metabolomic screening",
author = "Butikova, {Ekaterina A.} and Basov, {Nikita V.} and Rogachev, {Artem D.} and Gaisler, {Evgeniy V.} and Ivanisenko, {Vladimir A.} and Demenkov, {Pavel S.} and Makarova, {Aelita Luiza A.} and Ivanisenko, {Timofey V.} and Razumov, {Ivan A.} and Kolomeyets, {Daria A.} and Cheresiz, {Sergey V.} and Solovieva, {Olga I.} and Larionov, {Kirill P.} and Sotnikova, {Yulia S.} and Patrushev, {Yuri V.} and Kolchanov, {Nikolay A.} and Pokrovsky, {Andrey G.} and Vinokurov, {Nikolay A.} and Kanygin, {Vladimir V.} and Popik, {Vasiliy M.} and Shevchenko, {Oleg A.}",
note = "The experimental part of the study was carried out with the support of the Russian Science Foundation (Project No. 19-72-202) at the unique Novosibirsk Free Electron Laser installation using equipment from the Siberian Center for Synchrotron and Terahertz Radiation. The funds from the state project No. BC 2020-0039-1 have been used to conduct cell research. LC-MS/MS analysis has been conducted with the support of a state project No. FSUS-2020-0035. ",
year = "2025",
month = mar,
doi = "10.1016/j.bbalip.2025.159595",
language = "English",
volume = "1870",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1879-2618",
number = "2",

}

RIS

TY - JOUR

T1 - Metabolomic and gene networks approaches reveal the role of mitochondrial membrane proteins in response of human melanoma cells to THz radiation

AU - Butikova, Ekaterina A.

AU - Basov, Nikita V.

AU - Rogachev, Artem D.

AU - Gaisler, Evgeniy V.

AU - Ivanisenko, Vladimir A.

AU - Demenkov, Pavel S.

AU - Makarova, Aelita Luiza A.

AU - Ivanisenko, Timofey V.

AU - Razumov, Ivan A.

AU - Kolomeyets, Daria A.

AU - Cheresiz, Sergey V.

AU - Solovieva, Olga I.

AU - Larionov, Kirill P.

AU - Sotnikova, Yulia S.

AU - Patrushev, Yuri V.

AU - Kolchanov, Nikolay A.

AU - Pokrovsky, Andrey G.

AU - Vinokurov, Nikolay A.

AU - Kanygin, Vladimir V.

AU - Popik, Vasiliy M.

AU - Shevchenko, Oleg A.

N1 - The experimental part of the study was carried out with the support of the Russian Science Foundation (Project No. 19-72-202) at the unique Novosibirsk Free Electron Laser installation using equipment from the Siberian Center for Synchrotron and Terahertz Radiation. The funds from the state project No. BC 2020-0039-1 have been used to conduct cell research. LC-MS/MS analysis has been conducted with the support of a state project No. FSUS-2020-0035.

PY - 2025/3

Y1 - 2025/3

N2 - Terahertz (THz) radiation has gained attention due to technological advancements, but its biological effects remain unclear. We investigated the impact of 2.3 THz radiation on SK-MEL-28 cells using metabolomic and gene network analysis. Forty metabolites, primarily related to purine, pyrimidine synthesis and breakdown pathways, were significantly altered post-irradiation. Lipids, such as ceramides and phosphatidylcholines, were also affected. Gene network reconstruction and analysis identified key regulators of the enzymes involved in biosynthesis and degradation of significantly altered metabolites. Mitochondrial membrane components, such as the respiratory chain complex, the proton-transporting ATP synthase complex, and components of lipid rafts reacted to THz radiation. We propose that THz radiation induces reversible disruption of the lipid raft macromolecular structure, thereby altering mitochondrial molecule transport while maintaining protein integrity, which explains the high cell survival rate. Our findings enhance the understanding of THz biological effects and emphasize the role of membrane components in the cellular response to THz radiation.

AB - Terahertz (THz) radiation has gained attention due to technological advancements, but its biological effects remain unclear. We investigated the impact of 2.3 THz radiation on SK-MEL-28 cells using metabolomic and gene network analysis. Forty metabolites, primarily related to purine, pyrimidine synthesis and breakdown pathways, were significantly altered post-irradiation. Lipids, such as ceramides and phosphatidylcholines, were also affected. Gene network reconstruction and analysis identified key regulators of the enzymes involved in biosynthesis and degradation of significantly altered metabolites. Mitochondrial membrane components, such as the respiratory chain complex, the proton-transporting ATP synthase complex, and components of lipid rafts reacted to THz radiation. We propose that THz radiation induces reversible disruption of the lipid raft macromolecular structure, thereby altering mitochondrial molecule transport while maintaining protein integrity, which explains the high cell survival rate. Our findings enhance the understanding of THz biological effects and emphasize the role of membrane components in the cellular response to THz radiation.

KW - ANDSystem

KW - Biological effects of non-ionizing radiation

KW - Biophotonics

KW - Cell metabolomics

KW - Infrared radiation

KW - Lipid rafts

KW - Melanoma

KW - THz

KW - Targeted metabolomic screening

UR - https://www.mendeley.com/catalogue/2e953f30-1019-335c-b5ec-13c069ab07e4/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85215815043&origin=inward&txGid=f723b5cbf72f658a757ac6956ed61035

U2 - 10.1016/j.bbalip.2025.159595

DO - 10.1016/j.bbalip.2025.159595

M3 - Article

C2 - 39842507

VL - 1870

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1879-2618

IS - 2

M1 - 159595

ER -

ID: 63506364