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Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia. / Shekhovtsov, Sergei V.; Bulakhova, Nina A.; Tsentalovich, Yuri P. et al.

In: Scientific Reports, Vol. 10, No. 1, 14604, 03.09.2020.

Research output: Contribution to journal › Article › peer-review

Harvard

Shekhovtsov, SV, Bulakhova, NA, Tsentalovich, YP, Zelentsova, EA, Yanshole, LV, Meshcheryakova, EN & Berman, DI 2020, 'Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia', Scientific Reports, vol. 10, no. 1, 14604. https://doi.org/10.1038/s41598-020-71616-4

APA

Shekhovtsov, S. V., Bulakhova, N. A., Tsentalovich, Y. P., Zelentsova, E. A., Yanshole, L. V., Meshcheryakova, E. N., & Berman, D. I. (2020). Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia. Scientific Reports, 10(1), [14604]. https://doi.org/10.1038/s41598-020-71616-4

Vancouver

Shekhovtsov SV, Bulakhova NA, Tsentalovich YP, Zelentsova EA, Yanshole LV, Meshcheryakova EN et al. Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia. Scientific Reports. 2020 Sept 3;10(1):14604. doi: 10.1038/s41598-020-71616-4

Author

Shekhovtsov, Sergei V. ; Bulakhova, Nina A. ; Tsentalovich, Yuri P. et al. / Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia. In: Scientific Reports. 2020 ; Vol. 10, No. 1.

BibTeX

@article{0229841191e24d569569a53cbf9208d1,
title = "Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia",
abstract = "The Siberian wood frog Rana amurensis is a recently discovered example of extreme hypoxia tolerance that is able to survive several months without oxygen. We studied metabolomic profiles of heart and liver of R. amurensis exposed to 17 days of extreme hypoxia. Without oxygen, the studied tissues experience considerable stress with a drastic decrease of ATP, phosphocreatine, and NAD+ concentrations, and concomitant increase of AMP, creatine, and NADH. Heart and liver switch to different pathways of glycolysis with differential accumulation of lactate, alanine, succinate, as well as 2,3-butanediol (previously not reported for vertebrates as an end product of glycolysis) and depletion of aspartate. We also observed statistically significant changes in concentrations of certain osmolytes and choline-related compounds. Low succinate/fumarate ratio and high glutathione levels indicate adaptations to reoxygenation stress. Our data suggest that maintenance of the ATP/ADP pool is not required for survival of R. amurensis, in contrast to anoxia-tolerant turtles.",
keywords = "ANOXIA TOLERANCE, REPERFUSION INJURY, LC-MS, 2,3-BUTANEDIOL, GLYCEROL, SUCCINATE, ACCUMULATION, ISCHEMIA",
author = "Shekhovtsov, {Sergei V.} and Bulakhova, {Nina A.} and Tsentalovich, {Yuri P.} and Zelentsova, {Ekaterina A.} and Yanshole, {Lyudmila V.} and Meshcheryakova, {Ekaterina N.} and Berman, {Daniil I.}",
year = "2020",
month = sep,
day = "3",
doi = "10.1038/s41598-020-71616-4",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Metabolic response of the Siberian wood frog Rana amurensis to extreme hypoxia

AU - Shekhovtsov, Sergei V.

AU - Bulakhova, Nina A.

AU - Tsentalovich, Yuri P.

AU - Zelentsova, Ekaterina A.

AU - Yanshole, Lyudmila V.

AU - Meshcheryakova, Ekaterina N.

AU - Berman, Daniil I.

PY - 2020/9/3

Y1 - 2020/9/3

N2 - The Siberian wood frog Rana amurensis is a recently discovered example of extreme hypoxia tolerance that is able to survive several months without oxygen. We studied metabolomic profiles of heart and liver of R. amurensis exposed to 17 days of extreme hypoxia. Without oxygen, the studied tissues experience considerable stress with a drastic decrease of ATP, phosphocreatine, and NAD+ concentrations, and concomitant increase of AMP, creatine, and NADH. Heart and liver switch to different pathways of glycolysis with differential accumulation of lactate, alanine, succinate, as well as 2,3-butanediol (previously not reported for vertebrates as an end product of glycolysis) and depletion of aspartate. We also observed statistically significant changes in concentrations of certain osmolytes and choline-related compounds. Low succinate/fumarate ratio and high glutathione levels indicate adaptations to reoxygenation stress. Our data suggest that maintenance of the ATP/ADP pool is not required for survival of R. amurensis, in contrast to anoxia-tolerant turtles.

AB - The Siberian wood frog Rana amurensis is a recently discovered example of extreme hypoxia tolerance that is able to survive several months without oxygen. We studied metabolomic profiles of heart and liver of R. amurensis exposed to 17 days of extreme hypoxia. Without oxygen, the studied tissues experience considerable stress with a drastic decrease of ATP, phosphocreatine, and NAD+ concentrations, and concomitant increase of AMP, creatine, and NADH. Heart and liver switch to different pathways of glycolysis with differential accumulation of lactate, alanine, succinate, as well as 2,3-butanediol (previously not reported for vertebrates as an end product of glycolysis) and depletion of aspartate. We also observed statistically significant changes in concentrations of certain osmolytes and choline-related compounds. Low succinate/fumarate ratio and high glutathione levels indicate adaptations to reoxygenation stress. Our data suggest that maintenance of the ATP/ADP pool is not required for survival of R. amurensis, in contrast to anoxia-tolerant turtles.

KW - ANOXIA TOLERANCE

KW - REPERFUSION INJURY

KW - LC-MS

KW - 2,3-BUTANEDIOL

KW - GLYCEROL

KW - SUCCINATE

KW - ACCUMULATION

KW - ISCHEMIA

UR - http://www.scopus.com/inward/record.url?scp=85090123020&partnerID=8YFLogxK

U2 - 10.1038/s41598-020-71616-4

DO - 10.1038/s41598-020-71616-4

M3 - Article

C2 - 32884088

AN - SCOPUS:85090123020

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 14604

ER -

ID: 25292763