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Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate. / Fan, Haiyan; Dukenbayev, Kanat; Nurtay, Lazzat et al.

In: Journal of Inorganic Biochemistry, Vol. 258, 112619, 2024.

Research output: Contribution to journal › Article › peer-review

Harvard

Fan, H, Dukenbayev, K, Nurtay, L, Nazir, F, Daniyeva, N, Pham, TT & Benassi, E 2024, 'Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate', Journal of Inorganic Biochemistry, vol. 258, 112619. https://doi.org/10.1016/j.jinorgbio.2024.112619

APA

Fan, H., Dukenbayev, K., Nurtay, L., Nazir, F., Daniyeva, N., Pham, T. T., & Benassi, E. (2024). Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate. Journal of Inorganic Biochemistry, 258, [112619]. https://doi.org/10.1016/j.jinorgbio.2024.112619

Vancouver

Fan H, Dukenbayev K, Nurtay L, Nazir F, Daniyeva N, Pham TT et al. Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate. Journal of Inorganic Biochemistry. 2024;258:112619. doi: 10.1016/j.jinorgbio.2024.112619

Author

Fan, Haiyan ; Dukenbayev, Kanat ; Nurtay, Lazzat et al. / Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate. In: Journal of Inorganic Biochemistry. 2024 ; Vol. 258.

BibTeX

@article{369f9eba2d5e4896bbd888871298f04b,
title = "Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate",
abstract = "The present study describes a novel antimicrobial mechanism based on Sodium Orthovanadate (SOV), an alkaline phosphatase inhibitor. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were employed to examine the surface morphologies of the test organism, Escherichia coli (E. coli), during various antibacterial phases. Our results indicated that SOV kills bacteria by attacking cell wall growth and development, leaving E. coli's outer membrane intact. Our antimicrobial test indicated that the MIC of SOV for both E. coli and Lactococcus lactis (L. lactis) is 40 μM. A combination of quantum mechanical calculations and vibrational spectroscopy revealed that divanadate from SOV strongly coordinates with Ca2+ and Mg2+, which are the activity centers for the phosphatase that regulates bacterial cell wall synthesis. The current study is the first to propose the antibacterial mechanism caused by SOV attacking cell wall.",
keywords = "Alkaline phosphatase inhibitor, Antimicrobial, Quantum mechanical calculations, Sodium orthovanadate, Vibrational spectroscopy",
author = "Haiyan Fan and Kanat Dukenbayev and Lazzat Nurtay and Faisal Nazir and Nurgul Daniyeva and Pham, {Tri T.} and Enrico Benassi",
note = "This work was supported by Nazarbayev University Small Grants 11022021FD2928 (HF) and 021220FD4451 (TTP).",
year = "2024",
doi = "10.1016/j.jinorgbio.2024.112619",
language = "русский",
volume = "258",
journal = "Journal of Inorganic Biochemistry",
issn = "0162-0134",
publisher = "Elsevier Science Inc.",

}

RIS

TY - JOUR

T1 - Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate

AU - Fan, Haiyan

AU - Dukenbayev, Kanat

AU - Nurtay, Lazzat

AU - Nazir, Faisal

AU - Daniyeva, Nurgul

AU - Pham, Tri T.

AU - Benassi, Enrico

N1 - This work was supported by Nazarbayev University Small Grants 11022021FD2928 (HF) and 021220FD4451 (TTP).

PY - 2024

Y1 - 2024

N2 - The present study describes a novel antimicrobial mechanism based on Sodium Orthovanadate (SOV), an alkaline phosphatase inhibitor. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were employed to examine the surface morphologies of the test organism, Escherichia coli (E. coli), during various antibacterial phases. Our results indicated that SOV kills bacteria by attacking cell wall growth and development, leaving E. coli's outer membrane intact. Our antimicrobial test indicated that the MIC of SOV for both E. coli and Lactococcus lactis (L. lactis) is 40 μM. A combination of quantum mechanical calculations and vibrational spectroscopy revealed that divanadate from SOV strongly coordinates with Ca2+ and Mg2+, which are the activity centers for the phosphatase that regulates bacterial cell wall synthesis. The current study is the first to propose the antibacterial mechanism caused by SOV attacking cell wall.

AB - The present study describes a novel antimicrobial mechanism based on Sodium Orthovanadate (SOV), an alkaline phosphatase inhibitor. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were employed to examine the surface morphologies of the test organism, Escherichia coli (E. coli), during various antibacterial phases. Our results indicated that SOV kills bacteria by attacking cell wall growth and development, leaving E. coli's outer membrane intact. Our antimicrobial test indicated that the MIC of SOV for both E. coli and Lactococcus lactis (L. lactis) is 40 μM. A combination of quantum mechanical calculations and vibrational spectroscopy revealed that divanadate from SOV strongly coordinates with Ca2+ and Mg2+, which are the activity centers for the phosphatase that regulates bacterial cell wall synthesis. The current study is the first to propose the antibacterial mechanism caused by SOV attacking cell wall.

KW - Alkaline phosphatase inhibitor

KW - Antimicrobial

KW - Quantum mechanical calculations

KW - Sodium orthovanadate

KW - Vibrational spectroscopy

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85194547248&origin=inward&txGid=35255104a5494c89da0d138fcb414ed0

UR - https://www.mendeley.com/catalogue/946fe9a0-e525-36b2-9252-f5d19bbbe745/

U2 - 10.1016/j.jinorgbio.2024.112619

DO - 10.1016/j.jinorgbio.2024.112619

M3 - статья

C2 - 38823066

VL - 258

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

SN - 0162-0134

M1 - 112619

ER -

ID: 60849469