Research output: Contribution to journal › Article › peer-review
Locus-Specific Bisulfate NGS Sequencing of GSTP1, RNF219, and KIAA1539 Genes in the Total Pool of Cell-Free and Cell-Surface-Bound DNA in Prostate Cancer: A Novel Approach for Prostate Cancer Diagnostics. / Bryzgunova, Olga; Bondar, Anna; Ruzankin, Pavel et al.
In: Cancers, Vol. 15, No. 2, 431, 09.01.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Locus-Specific Bisulfate NGS Sequencing of GSTP1, RNF219, and KIAA1539 Genes in the Total Pool of Cell-Free and Cell-Surface-Bound DNA in Prostate Cancer: A Novel Approach for Prostate Cancer Diagnostics
AU - Bryzgunova, Olga
AU - Bondar, Anna
AU - Ruzankin, Pavel
AU - Tarasenko, Anton
AU - Zaripov, Marat
AU - Kabilov, Marsel
AU - Laktionov, Pavel
N1 - Funding: The study of O.B., A.B., M.Z., M.K. and P.L. was supported by the Russian state budget project via the ICBFM SB RAS No. 121030200173-6; the study of A.T. and P.R. was supported by the Program for fundamental scientific research of the Siberian Branch of the Russian Academy of Sciences, project FWNF-2022-0010.
PY - 2023/1/9
Y1 - 2023/1/9
N2 - The locus-specific methylation of three genes (GSTP1, RNF219, and KIAA1539, also known as FAM214B) in the total pool of blood cell-free DNA, including cell-free DNA from plasma and cell-surface-bound DNA, of patients with prostate cancer and healthy donors was studied on the MiSeq platform. Our study found a higher methylation index of loci for total cell-free DNA compared with cell-free DNA. For total cell-free DNA, the methylation of GSTP1 in each of the 11 positions provided a complete separation of cancer patients from healthy donors, whereas for cell-free DNA, there were no positions in the three genes allowing for such separation. Among the prostate cancer patients, the minimum proportion of GSTP1 genes methylated in any of the 17 positions was 12.1% of the total circulated DNA fragments, and the minimum proportion of GSTP1 genes methylated in any of the 11 diagnostically specific positions was 8.4%. Total cell-free DNA was shown to be more convenient and informative as a source of methylated DNA molecules circulating in the blood than cell-free DNA.
AB - The locus-specific methylation of three genes (GSTP1, RNF219, and KIAA1539, also known as FAM214B) in the total pool of blood cell-free DNA, including cell-free DNA from plasma and cell-surface-bound DNA, of patients with prostate cancer and healthy donors was studied on the MiSeq platform. Our study found a higher methylation index of loci for total cell-free DNA compared with cell-free DNA. For total cell-free DNA, the methylation of GSTP1 in each of the 11 positions provided a complete separation of cancer patients from healthy donors, whereas for cell-free DNA, there were no positions in the three genes allowing for such separation. Among the prostate cancer patients, the minimum proportion of GSTP1 genes methylated in any of the 17 positions was 12.1% of the total circulated DNA fragments, and the minimum proportion of GSTP1 genes methylated in any of the 11 diagnostically specific positions was 8.4%. Total cell-free DNA was shown to be more convenient and informative as a source of methylated DNA molecules circulating in the blood than cell-free DNA.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85146742642&origin=inward&txGid=b723f60ea60f27fb8cbbfba78037431e
UR - https://www.mendeley.com/catalogue/9d3273b6-c92b-3ef6-a58f-42f2399ac3ba/
U2 - 10.3390/cancers15020431
DO - 10.3390/cancers15020431
M3 - Article
C2 - 36672380
VL - 15
JO - Cancers
JF - Cancers
SN - 2072-6694
IS - 2
M1 - 431
ER -
ID: 43662584