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Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution. / Morozova, Olga B.; Fishman, Natalya N.; Yurkovskaya, Alexandra V.

In: ChemPhysChem, Vol. 19, No. 20, 19.10.2018, p. 2696-2702.

Research output: Contribution to journal › Article › peer-review

Harvard

Morozova, OB, Fishman, NN & Yurkovskaya, AV 2018, 'Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution', ChemPhysChem, vol. 19, no. 20, pp. 2696-2702. https://doi.org/10.1002/cphc.201800539

APA

Morozova, O. B., Fishman, N. N., & Yurkovskaya, A. V. (2018). Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution. ChemPhysChem, 19(20), 2696-2702. https://doi.org/10.1002/cphc.201800539

Vancouver

Morozova OB, Fishman NN, Yurkovskaya AV. Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution. ChemPhysChem. 2018 Oct 19;19(20):2696-2702. doi: 10.1002/cphc.201800539

Author

Morozova, Olga B. ; Fishman, Natalya N. ; Yurkovskaya, Alexandra V. / Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution. In: ChemPhysChem. 2018 ; Vol. 19, No. 20. pp. 2696-2702.

BibTeX

@article{0cc865d77f6042b087cb86042fa12851,
title = "Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution",
abstract = "Time-resolved chemically induced dynamic nuclear polarization (TR-CIDNP) is applied to follow transformation of the short-lived neutral guanine radical into a secondary guanine radical by its protonation, presumably at position N7. In the initial step the photoreaction of guanosine-5′-monophosphate (GMP) with triplet excited 3,3′,4,4′-tetracarboxy benzophenone (TCBP) leads to formation of the neutral radical G(−H).. The evidence of the radical conversion is based on the inversion of CIDNP sign for TCBP and GMP protons on the microsecond timescale as a result of the change in magnetic resonance parameters in the pairs of TCBP and GMP radicals due to structural changes of the GMP radical. Acceleration of the CIDNP sign change upon addition of phosphate (proton donor) confirms that the radical transformation responsible for the observed CIDNP kinetics is protonation of the neutral guanine radical with formation of the newly characterized cation radical, (G.+)′. From the full analysis of the pH-dependent CIDNP kinetics, the protonation and deprotonation behaviour is quantitatively characterized, giving pKa=8.0±0.2 of the cation radical (G.+)′.",
keywords = "DNA oxidation, guanine radical, NMR spectroscopy, photo-oxidation, photochemistry",
author = "Morozova, {Olga B.} and Fishman, {Natalya N.} and Yurkovskaya, {Alexandra V.}",
note = "Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2018",
month = oct,
day = "19",
doi = "10.1002/cphc.201800539",
language = "English",
volume = "19",
pages = "2696--2702",
journal = "ChemPhysChem",
issn = "1439-4235",
publisher = "Wiley-Blackwell",
number = "20",

}

RIS

TY - JOUR

T1 - Kinetics of Reversible Protonation of Transient Neutral Guanine Radical in Neutral Aqueous Solution

AU - Morozova, Olga B.

AU - Fishman, Natalya N.

AU - Yurkovskaya, Alexandra V.

N1 - Publisher Copyright: © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2018/10/19

Y1 - 2018/10/19

N2 - Time-resolved chemically induced dynamic nuclear polarization (TR-CIDNP) is applied to follow transformation of the short-lived neutral guanine radical into a secondary guanine radical by its protonation, presumably at position N7. In the initial step the photoreaction of guanosine-5′-monophosphate (GMP) with triplet excited 3,3′,4,4′-tetracarboxy benzophenone (TCBP) leads to formation of the neutral radical G(−H).. The evidence of the radical conversion is based on the inversion of CIDNP sign for TCBP and GMP protons on the microsecond timescale as a result of the change in magnetic resonance parameters in the pairs of TCBP and GMP radicals due to structural changes of the GMP radical. Acceleration of the CIDNP sign change upon addition of phosphate (proton donor) confirms that the radical transformation responsible for the observed CIDNP kinetics is protonation of the neutral guanine radical with formation of the newly characterized cation radical, (G.+)′. From the full analysis of the pH-dependent CIDNP kinetics, the protonation and deprotonation behaviour is quantitatively characterized, giving pKa=8.0±0.2 of the cation radical (G.+)′.

AB - Time-resolved chemically induced dynamic nuclear polarization (TR-CIDNP) is applied to follow transformation of the short-lived neutral guanine radical into a secondary guanine radical by its protonation, presumably at position N7. In the initial step the photoreaction of guanosine-5′-monophosphate (GMP) with triplet excited 3,3′,4,4′-tetracarboxy benzophenone (TCBP) leads to formation of the neutral radical G(−H).. The evidence of the radical conversion is based on the inversion of CIDNP sign for TCBP and GMP protons on the microsecond timescale as a result of the change in magnetic resonance parameters in the pairs of TCBP and GMP radicals due to structural changes of the GMP radical. Acceleration of the CIDNP sign change upon addition of phosphate (proton donor) confirms that the radical transformation responsible for the observed CIDNP kinetics is protonation of the neutral guanine radical with formation of the newly characterized cation radical, (G.+)′. From the full analysis of the pH-dependent CIDNP kinetics, the protonation and deprotonation behaviour is quantitatively characterized, giving pKa=8.0±0.2 of the cation radical (G.+)′.

KW - DNA oxidation

KW - guanine radical

KW - NMR spectroscopy

KW - photo-oxidation

KW - photochemistry

UR - http://www.scopus.com/inward/record.url?scp=85055154096&partnerID=8YFLogxK

U2 - 10.1002/cphc.201800539

DO - 10.1002/cphc.201800539

M3 - Article

C2 - 29978943

AN - SCOPUS:85055154096

VL - 19

SP - 2696

EP - 2702

JO - ChemPhysChem

JF - ChemPhysChem

SN - 1439-4235

IS - 20

ER -

ID: 17247146