Key thermodynamic characteristics of Cas9 and Cas12a endonucleases’ cleavage of a DNA substrate containing a nucleotide mismatch in the region complementary to RNA

Research output: Contribution to journal › Article › peer-review

Original language	English
Article number	151892
Pages (from-to)	151892
Journal	Biochemical and Biophysical Research Communications
Volume	768
DOIs	https://doi.org/10.1016/j.bbrc.2025.151892
Publication status	Published - 1 Jul 2025

CRISPR/Cas, Cas12a activity, Cleavage, Molecular dynamics simulation, Oligonucleotide mismatch, Thermodynamics, DNA/metabolism, Substrate Specificity, Base Pair Mismatch, CRISPR-Associated Proteins/metabolism, Molecular Dynamics Simulation, Bacterial Proteins/metabolism, RNA/metabolism, CRISPR-Associated Protein 9/metabolism, CRISPR-Cas Systems, Kinetics, Endodeoxyribonucleases/metabolism, DNA Cleavage, RNA, Guide, CRISPR-Cas Systems/metabolism

ID: 66182160