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Key aspects of nucleic acid library design for in vitro selection. / Vorobyeva, Maria A.; Davydova, Anna S.; Vorobjev, Pavel E. et al.

In: International Journal of Molecular Sciences, Vol. 19, No. 2, 470, 05.02.2018.

Research output: Contribution to journal › Review article › peer-review

Harvard

Vorobyeva, MA, Davydova, AS, Vorobjev, PE, Pyshnyi, DV & Venyaminova, AG 2018, 'Key aspects of nucleic acid library design for in vitro selection', International Journal of Molecular Sciences, vol. 19, no. 2, 470. https://doi.org/10.3390/ijms19020470

APA

Vorobyeva, M. A., Davydova, A. S., Vorobjev, P. E., Pyshnyi, D. V., & Venyaminova, A. G. (2018). Key aspects of nucleic acid library design for in vitro selection. International Journal of Molecular Sciences, 19(2), [470]. https://doi.org/10.3390/ijms19020470

Vancouver

Vorobyeva MA, Davydova AS, Vorobjev PE, Pyshnyi DV, Venyaminova AG. Key aspects of nucleic acid library design for in vitro selection. International Journal of Molecular Sciences. 2018 Feb 5;19(2):470. doi: 10.3390/ijms19020470

Author

Vorobyeva, Maria A. ; Davydova, Anna S. ; Vorobjev, Pavel E. et al. / Key aspects of nucleic acid library design for in vitro selection. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 2.

BibTeX

@article{0dba85825d844a63bde609ee6ae00778,
title = "Key aspects of nucleic acid library design for in vitro selection",
abstract = "Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been established as powerful and tunable tools for biospecific applications, be it therapeutics, drug delivery systems or biosensors. It is now generally acknowledged that in vitro selection enables one to generate aptamers to almost any target of interest. However, the success of selection and the affinity of the resulting aptamers depend to a large extent on the nature and design of an initial random nucleic acid library. In this review, we summarize and discuss the most important features of the design of nucleic acid libraries for in vitro selection such as the nature of the library (DNA, RNA or modified nucleotides), the length of a randomized region and the presence of fixed sequences. We also compare and contrast different randomization strategies and consider computer methods of library design and some other aspects.",
keywords = "Aptamers, Design of nucleic acid libraries, SELEX, BINDING RNA, STRANDED-DNA APTAMERS, PROTEIN, RECOGNITION, HIGH-AFFINITY, STRUCTURED RNA POOLS, EVOLUTION, PRIMER, CAPTURE-SELEX, aptamers, design of nucleic acid libraries, GENOMIC SELEX",
author = "Vorobyeva, {Maria A.} and Davydova, {Anna S.} and Vorobjev, {Pavel E.} and Pyshnyi, {Dmitrii V.} and Venyaminova, {Alya G.}",
year = "2018",
month = feb,
day = "5",
doi = "10.3390/ijms19020470",
language = "English",
volume = "19",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

RIS

TY - JOUR

T1 - Key aspects of nucleic acid library design for in vitro selection

AU - Vorobyeva, Maria A.

AU - Davydova, Anna S.

AU - Vorobjev, Pavel E.

AU - Pyshnyi, Dmitrii V.

AU - Venyaminova, Alya G.

PY - 2018/2/5

Y1 - 2018/2/5

N2 - Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been established as powerful and tunable tools for biospecific applications, be it therapeutics, drug delivery systems or biosensors. It is now generally acknowledged that in vitro selection enables one to generate aptamers to almost any target of interest. However, the success of selection and the affinity of the resulting aptamers depend to a large extent on the nature and design of an initial random nucleic acid library. In this review, we summarize and discuss the most important features of the design of nucleic acid libraries for in vitro selection such as the nature of the library (DNA, RNA or modified nucleotides), the length of a randomized region and the presence of fixed sequences. We also compare and contrast different randomization strategies and consider computer methods of library design and some other aspects.

AB - Nucleic acid aptamers capable of selectively recognizing their target molecules have nowadays been established as powerful and tunable tools for biospecific applications, be it therapeutics, drug delivery systems or biosensors. It is now generally acknowledged that in vitro selection enables one to generate aptamers to almost any target of interest. However, the success of selection and the affinity of the resulting aptamers depend to a large extent on the nature and design of an initial random nucleic acid library. In this review, we summarize and discuss the most important features of the design of nucleic acid libraries for in vitro selection such as the nature of the library (DNA, RNA or modified nucleotides), the length of a randomized region and the presence of fixed sequences. We also compare and contrast different randomization strategies and consider computer methods of library design and some other aspects.

KW - Aptamers

KW - Design of nucleic acid libraries

KW - SELEX

KW - BINDING RNA

KW - STRANDED-DNA APTAMERS

KW - PROTEIN

KW - RECOGNITION

KW - HIGH-AFFINITY

KW - STRUCTURED RNA POOLS

KW - EVOLUTION

KW - PRIMER

KW - CAPTURE-SELEX

KW - aptamers

KW - design of nucleic acid libraries

KW - GENOMIC SELEX

UR - http://www.scopus.com/inward/record.url?scp=85041543400&partnerID=8YFLogxK

U2 - 10.3390/ijms19020470

DO - 10.3390/ijms19020470

M3 - Review article

C2 - 29401748

AN - SCOPUS:85041543400

VL - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 2

M1 - 470

ER -

ID: 10428200