Standard

Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer. / Tamkovich, S. N.; Somov, A. K.; Karpukhina, K. V. et al.

PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA): Interdisciplinary Problems and Clinical Applications - Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017. ed. / EY Gutmanas; OB Naimark; YP Sharkeev. Vol. 1882 American Institute of Physics Inc., 2017. 020075 (AIP Conference Proceedings; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Harvard

Tamkovich, SN, Somov, AK, Karpukhina, KV, Grigor'Eva, AE, Yunusova, NV, Stakheeva, MN, Voytsitskiy, VE, Kondakova, IV & Laktionov, PP 2017, Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer. in EY Gutmanas, OB Naimark & YP Sharkeev (eds), PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA): Interdisciplinary Problems and Clinical Applications - Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017. vol. 1882, 020075, AIP Conference Proceedings, vol. 1882, American Institute of Physics Inc., International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017, Tomsk, Russian Federation, 23.05.2017. https://doi.org/10.1063/1.5001654

APA

Tamkovich, S. N., Somov, A. K., Karpukhina, K. V., Grigor'Eva, A. E., Yunusova, N. V., Stakheeva, M. N., Voytsitskiy, V. E., Kondakova, I. V., & Laktionov, P. P. (2017). Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer. In EY. Gutmanas, OB. Naimark, & YP. Sharkeev (Eds.), PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA): Interdisciplinary Problems and Clinical Applications - Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 (Vol. 1882). [020075] (AIP Conference Proceedings; Vol. 1882). American Institute of Physics Inc.. https://doi.org/10.1063/1.5001654

Vancouver

Tamkovich SN, Somov AK, Karpukhina KV, Grigor'Eva AE, Yunusova NV, Stakheeva MN et al. Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer. In Gutmanas EY, Naimark OB, Sharkeev YP, editors, PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA): Interdisciplinary Problems and Clinical Applications - Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017. Vol. 1882. American Institute of Physics Inc. 2017. 020075. (AIP Conference Proceedings). doi: 10.1063/1.5001654

Author

Tamkovich, S. N. ; Somov, A. K. ; Karpukhina, K. V. et al. / Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer. PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA): Interdisciplinary Problems and Clinical Applications - Proceedings of the International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017. editor / EY Gutmanas ; OB Naimark ; YP Sharkeev. Vol. 1882 American Institute of Physics Inc., 2017. (AIP Conference Proceedings).

BibTeX

@inproceedings{6e0dc889131b46c7934a420dac939ee1,
title = "Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer",
abstract = "The exosomes containing tumor-specific protein represent a valuable source of material for the non-invasive diagnosis of malignant neoplasms. The exosomes from plasma and cell-associated exosomes from blood of healthy women, the patients with mastopathy and breast cancer patients were studied with the nanoparticle tracking analysis, transmission electron microscopy, flow cytometry and protein profiling. The exposure of CD63, CD24, CD9, and CD81 demonstrates isolation of mainly exosomes. A major part of exosomes in the blood of healthy women, patients with mastopathy and breast cancer patients is shown to be associated with the surface of blood cells. The concentration of the exosomes in the plasma of the breast cancer patients is higher than in the healthy women's plasma. The proteins ranged from 10 to 250 kDa were found in the exosomes by gradient SDS PAAGE; 8 regions of electrophoregram differ between healthy and illness patients in the expression level and number of proteins. These proteins are planned to be identified by MALDI TOF and studied as potential breast cancer markers.",
author = "Tamkovich, {S. N.} and Somov, {A. K.} and Karpukhina, {K. V.} and Grigor'Eva, {A. E.} and Yunusova, {N. V.} and Stakheeva, {M. N.} and Voytsitskiy, {V. E.} and Kondakova, {I. V.} and Laktionov, {P. P.}",
year = "2017",
month = sep,
day = "28",
doi = "10.1063/1.5001654",
language = "English",
volume = "1882",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "EY Gutmanas and OB Naimark and YP Sharkeev",
booktitle = "PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA)",
note = "International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017 ; Conference date: 23-05-2017 Through 26-05-2017",

}

RIS

TY - GEN

T1 - Isolation and characterization of exosomes from blood of patients with mastopathy and breast cancer

AU - Tamkovich, S. N.

AU - Somov, A. K.

AU - Karpukhina, K. V.

AU - Grigor'Eva, A. E.

AU - Yunusova, N. V.

AU - Stakheeva, M. N.

AU - Voytsitskiy, V. E.

AU - Kondakova, I. V.

AU - Laktionov, P. P.

PY - 2017/9/28

Y1 - 2017/9/28

N2 - The exosomes containing tumor-specific protein represent a valuable source of material for the non-invasive diagnosis of malignant neoplasms. The exosomes from plasma and cell-associated exosomes from blood of healthy women, the patients with mastopathy and breast cancer patients were studied with the nanoparticle tracking analysis, transmission electron microscopy, flow cytometry and protein profiling. The exposure of CD63, CD24, CD9, and CD81 demonstrates isolation of mainly exosomes. A major part of exosomes in the blood of healthy women, patients with mastopathy and breast cancer patients is shown to be associated with the surface of blood cells. The concentration of the exosomes in the plasma of the breast cancer patients is higher than in the healthy women's plasma. The proteins ranged from 10 to 250 kDa were found in the exosomes by gradient SDS PAAGE; 8 regions of electrophoregram differ between healthy and illness patients in the expression level and number of proteins. These proteins are planned to be identified by MALDI TOF and studied as potential breast cancer markers.

AB - The exosomes containing tumor-specific protein represent a valuable source of material for the non-invasive diagnosis of malignant neoplasms. The exosomes from plasma and cell-associated exosomes from blood of healthy women, the patients with mastopathy and breast cancer patients were studied with the nanoparticle tracking analysis, transmission electron microscopy, flow cytometry and protein profiling. The exposure of CD63, CD24, CD9, and CD81 demonstrates isolation of mainly exosomes. A major part of exosomes in the blood of healthy women, patients with mastopathy and breast cancer patients is shown to be associated with the surface of blood cells. The concentration of the exosomes in the plasma of the breast cancer patients is higher than in the healthy women's plasma. The proteins ranged from 10 to 250 kDa were found in the exosomes by gradient SDS PAAGE; 8 regions of electrophoregram differ between healthy and illness patients in the expression level and number of proteins. These proteins are planned to be identified by MALDI TOF and studied as potential breast cancer markers.

UR - http://www.scopus.com/inward/record.url?scp=85041667712&partnerID=8YFLogxK

U2 - 10.1063/1.5001654

DO - 10.1063/1.5001654

M3 - Conference contribution

AN - SCOPUS:85041667712

VL - 1882

T3 - AIP Conference Proceedings

BT - PHYSICS OF CANCER: INTERDISCIPLINARY PROBLEMS AND CLINICAL APPLICATIONS (PC IPCA)

A2 - Gutmanas, EY

A2 - Naimark, OB

A2 - Sharkeev, YP

PB - American Institute of Physics Inc.

T2 - International Conference on Physics of Cancer: Interdisciplinary Problems and Clinical Applications, PC IPCA 2017

Y2 - 23 May 2017 through 26 May 2017

ER -

ID: 9641196