TY - JOUR

T1 - Intensive cultivation of Salileptolyngbya sp. IBSS-CYA-8 on microcarriers in a suspended-solid phase photobioreactor

AU - Miroshnichenko, E. S.

AU - Zheleznova, S. N.

AU - Ignateva, D. A.

AU - Naumov, I. V.

AU - Dimov, S. V.

AU - Kapranov, S. V.

AU - Gevorgiz, R. G.

N1 - Intensive cultivation of Salileptolyngbya sp. IBSS-CYA-8 on microcarriers in a suspended-solid phase photobioreactor / E. S. Miroshnichenko, S. N. Zheleznova, D. A. Ignateva [et al.] // Journal of Applied Phycology. – 2026. – DOI 10.1007/s10811-025-03781-w. – EDN CXTECZ. This study was conducted according to the topics of the governmental assignment to the A.O. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, # 124022400152-1 (Comprehensive study of the mechanisms of functioning of marine biotechnological complexes for obtaining biologically active substances from aquatic organisms) and # 124030100030-0 (Studying the features of the functioning and dynamics of subtropical and tropical coastal ecosystems under the climate change and anthropogenic load using remote sensing, cloud information processing, and machine learning to create a scientific basis for their rational use), and the financial support for this study provided by the Russian Science Foundation (grant No 25-79-30002).

PY - 2026/2

Y1 - 2026/2

N2 - Marine filamentous cyanobacteria are a promising source of bioactive compounds, yet their large-scale cultivation remains challenging due to low cell density and light limitations. This study focuses on the intensive cultivation of Salileptolyngbya sp. IBSS-CYA-8 using microcarriers in a suspended-solid phase photobioreactor. The strain was isolated from the Black Sea and identified through morphological and phylogenetic analyses as a new species. Cultivation experiments employed polyamide particles (5-µm, 50-µm, 1000-µm) and silicon dioxide sorbent Polisorb MP as microcarriers to promote biofilm formation. A modified BG-11-M medium with stoichiometrically adjusted nutrient ratios was used to support intensive growth. Biomass productivity was assessed using an original technique based on monitoring inorganic phosphorus uptake and biochemical analyses were used to quantify proteins, lipids, carbohydrates, and phycobiliproteins. Results demonstrated that microcarriers significantly improved biomass concentration (3.0±0.04 g DW L-1), with high productivity: 0.32 g DW L-1 day-1 on Polisorb MP and 0.30 g DW L-1 day-1 on polyamide 50-µm particles. The modified BG-11-M medium increased biomass output compared to the standard BG-11, with protein and lipid contents reaching 56% and 25% of dry weight, respectively. Notably, the strain exhibited high phycoerythrin production (125.8 mg g-1 DW and 37.7 mg L-1 day-1), surpassing many commercial red algae. The study highlights the efficacy of microcarriers and optimized media for scalable cyanobacterial cultivation, positioning Salileptolyngbya sp. IBSS-CYA-8 as a promising candidate for various industrial applications.

AB - Marine filamentous cyanobacteria are a promising source of bioactive compounds, yet their large-scale cultivation remains challenging due to low cell density and light limitations. This study focuses on the intensive cultivation of Salileptolyngbya sp. IBSS-CYA-8 using microcarriers in a suspended-solid phase photobioreactor. The strain was isolated from the Black Sea and identified through morphological and phylogenetic analyses as a new species. Cultivation experiments employed polyamide particles (5-µm, 50-µm, 1000-µm) and silicon dioxide sorbent Polisorb MP as microcarriers to promote biofilm formation. A modified BG-11-M medium with stoichiometrically adjusted nutrient ratios was used to support intensive growth. Biomass productivity was assessed using an original technique based on monitoring inorganic phosphorus uptake and biochemical analyses were used to quantify proteins, lipids, carbohydrates, and phycobiliproteins. Results demonstrated that microcarriers significantly improved biomass concentration (3.0±0.04 g DW L-1), with high productivity: 0.32 g DW L-1 day-1 on Polisorb MP and 0.30 g DW L-1 day-1 on polyamide 50-µm particles. The modified BG-11-M medium increased biomass output compared to the standard BG-11, with protein and lipid contents reaching 56% and 25% of dry weight, respectively. Notably, the strain exhibited high phycoerythrin production (125.8 mg g-1 DW and 37.7 mg L-1 day-1), surpassing many commercial red algae. The study highlights the efficacy of microcarriers and optimized media for scalable cyanobacterial cultivation, positioning Salileptolyngbya sp. IBSS-CYA-8 as a promising candidate for various industrial applications.

KW - Biochemical composition

KW - Biofilm cultivation

KW - Marine cyanobacteria, Salileptolyngbya

KW - Microcarriers

UR - https://www.scopus.com/pages/publications/105029871440

UR - https://elibrary.ru/item.asp?id=88978818

UR - https://www.mendeley.com/catalogue/617b33b2-83bb-3bd5-9d9b-fc4b6152afff/

U2 - 10.1007/s10811-025-03781-w

DO - 10.1007/s10811-025-03781-w

M3 - Article

VL - 38

SP - 141

EP - 159

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

IS - 1

ER -