Research output: Contribution to journal › Article › peer-review
Integrative Study of the Life Cycle in the Marine Protist Thraustochytrium aureum ssp. strugatskii. / Doroshkov, Alexey V.; Naumenko, Ludmila G.; Iukhtanov, Daniil A. et al.
In: International Journal of Molecular Sciences, Vol. 26, No. 23, 11302, 22.11.2025.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Integrative Study of the Life Cycle in the Marine Protist Thraustochytrium aureum ssp. strugatskii
AU - Doroshkov, Alexey V.
AU - Naumenko, Ludmila G.
AU - Iukhtanov, Daniil A.
AU - Morozova, Ksenia N.
AU - Kiseleva, Elena V.
AU - Menzorov, Aleksei G.
AU - Zubairova, Ulyana S.
N1 - This research was funded by Russian Science Foundation, grant number 24-24-00451 (https://rscf.ru/project/24-24-00451/, accessed on 25 October 2025). The APC was funded by Russian Science Foundation, grant number 24-24-00451.
PY - 2025/11/22
Y1 - 2025/11/22
N2 - Thraustochytrium aureum ssp. strugatskii, a marine protist belonging to the class Labyrinthulea, exhibits a complex life cycle characterized by alternating motile and vegetative phases. Using an integrative multimodal microscopy approach, we reconstructed its full developmental cycle and analyzed the coordination between cellular morphology, subcellular architecture, and population-level behavior. Transmission and scanning electron microscopy, combined with fluorescence and time-lapse imaging, revealed the dynamics of nuclear division, organelle rearrangement, and zoospore formation. Morphometric analysis of serial ultrathin sections demonstrated distinct changes in mitochondrial distribution, Golgi apparatus, and lipid droplet abundance during transitions between stages. We have shown that vegetative cells undergo synchronized karyokinesis coupled with stable nuclear-to-cytoplasmic ratios, leading to the emergence of multinucleate stages prior to zoospore formation. The integration of ultrastructural and dynamic data enabled us to propose a systems-level model linking metabolic state, morphogenesis, and population structure. This model highlights feedback regulation between nutrient availability, biomass accumulation, and developmental synchronization. Our results establish that T. aureum ssp. strugatskii has good potential to serve as a tractable model organism for systems-level studies of protists and provide an initial framework for predictive modeling of its life cycle under controlled conditions.
AB - Thraustochytrium aureum ssp. strugatskii, a marine protist belonging to the class Labyrinthulea, exhibits a complex life cycle characterized by alternating motile and vegetative phases. Using an integrative multimodal microscopy approach, we reconstructed its full developmental cycle and analyzed the coordination between cellular morphology, subcellular architecture, and population-level behavior. Transmission and scanning electron microscopy, combined with fluorescence and time-lapse imaging, revealed the dynamics of nuclear division, organelle rearrangement, and zoospore formation. Morphometric analysis of serial ultrathin sections demonstrated distinct changes in mitochondrial distribution, Golgi apparatus, and lipid droplet abundance during transitions between stages. We have shown that vegetative cells undergo synchronized karyokinesis coupled with stable nuclear-to-cytoplasmic ratios, leading to the emergence of multinucleate stages prior to zoospore formation. The integration of ultrastructural and dynamic data enabled us to propose a systems-level model linking metabolic state, morphogenesis, and population structure. This model highlights feedback regulation between nutrient availability, biomass accumulation, and developmental synchronization. Our results establish that T. aureum ssp. strugatskii has good potential to serve as a tractable model organism for systems-level studies of protists and provide an initial framework for predictive modeling of its life cycle under controlled conditions.
KW - Thraustochytrium aureum ssp. strugatskii
KW - Labyrinthulea
KW - life cycle
KW - multimodal microscopy
KW - ultrastructure
KW - morphometric analysis
KW - karyokinesis
KW - population dynamics
KW - biotechnology
KW - marine protists
UR - https://www.scopus.com/pages/publications/105024653471
UR - https://www.mendeley.com/catalogue/ae18a473-5735-3501-8c70-814bf516fa66/
U2 - 10.3390/ijms262311302
DO - 10.3390/ijms262311302
M3 - Article
C2 - 41373465
VL - 26
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 23
M1 - 11302
ER -
ID: 72845685