Research output: Contribution to journal › Article › peer-review
Influence of Cellular Lipids on Cryopreservation of Mammalian Oocytes and Preimplantation Embryos : A Review. / Amstislavsky, Sergei; Mokrousova, Valentina; Brusentsev, Eugeny et al.
In: Biopreservation and Biobanking, Vol. 17, No. 1, 01.02.2019, p. 76-83.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Influence of Cellular Lipids on Cryopreservation of Mammalian Oocytes and Preimplantation Embryos
T2 - A Review
AU - Amstislavsky, Sergei
AU - Mokrousova, Valentina
AU - Brusentsev, Eugeny
AU - Okotrub, Konstantin
AU - Comizzoli, Pierre
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Lipids are among the most abundant and essential cell components. Specifically, cytoplasmic lipid droplets (LDs) play crucial roles in cellular energy homeostasis. The foci of this review are (1) the composition and roles of lipids during oocyte maturation and early embryonic development, (2) possible causes of cryoinjuries in lipid-rich oocytes/embryos, and (3) ways to overcome these detrimental effects. Recent reports show that LDs in oocytes and embryos are not only energy depots but also are active organelles, possessing many other functions. In addition, analysis of the current literature confirms that lipid phase transition followed by phase separation during cryopreservation is one of the major causes of cryodamage in lipid-rich oocytes and embryos. While LDs and cell membranes are sensitive to chilling and freezing conditions, recent advances in vitrification and delipidation of lipid-rich oocytes and embryos partly mitigate cryodamage. The multidisciplinary approach is critical to reveal mechanisms underlying cryodamage and provides a theoretical basis for optimal cryopreservation of lipid-rich oocytes/embryos.
AB - Lipids are among the most abundant and essential cell components. Specifically, cytoplasmic lipid droplets (LDs) play crucial roles in cellular energy homeostasis. The foci of this review are (1) the composition and roles of lipids during oocyte maturation and early embryonic development, (2) possible causes of cryoinjuries in lipid-rich oocytes/embryos, and (3) ways to overcome these detrimental effects. Recent reports show that LDs in oocytes and embryos are not only energy depots but also are active organelles, possessing many other functions. In addition, analysis of the current literature confirms that lipid phase transition followed by phase separation during cryopreservation is one of the major causes of cryodamage in lipid-rich oocytes and embryos. While LDs and cell membranes are sensitive to chilling and freezing conditions, recent advances in vitrification and delipidation of lipid-rich oocytes and embryos partly mitigate cryodamage. The multidisciplinary approach is critical to reveal mechanisms underlying cryodamage and provides a theoretical basis for optimal cryopreservation of lipid-rich oocytes/embryos.
KW - cryopreservation
KW - Lipid droplets
KW - oocytes
KW - preimplantation embryo
KW - CLOSED-SYSTEM
KW - FATTY-ACID-COMPOSITION
KW - PHASE-TRANSITIONS
KW - RAMAN-SPECTROSCOPY
KW - MOUSE EMBRYOS
KW - DEVELOPMENTAL COMPETENCE
KW - BOVINE-OOCYTES
KW - lipid droplets
KW - PORCINE EMBRYOS
KW - IN-VITRO FERTILIZATION
KW - CHILLING SENSITIVITY
UR - http://www.scopus.com/inward/record.url?scp=85061602385&partnerID=8YFLogxK
U2 - 10.1089/bio.2018.0039
DO - 10.1089/bio.2018.0039
M3 - Article
C2 - 30256133
AN - SCOPUS:85061602385
VL - 17
SP - 76
EP - 83
JO - Biopreservation and Biobanking
JF - Biopreservation and Biobanking
SN - 1947-5535
IS - 1
ER -
ID: 18562846