TY - JOUR

T1 - In vivo stability of polyurethane-based electrospun vascular grafts in terms of chemistry and mechanics

AU - Gostev, Alexander A.

AU - Shundrina, Inna K.

AU - Pastukhov, Vitaliy I.

AU - Shutov, Alexey V.

AU - Chernonosova, Vera S.

AU - Karpenko, Andrey A.

AU - Laktionov, Pavel P.

N1 - Publisher Copyright: © 2020 by the authors. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/4/1

Y1 - 2020/4/1

N2 - The biostability of the polyurethanes Tecoflex EG-80A and Pellethane 2363-80A, used as basic polymers of the vascular grafts (VGs) produced by electrospinning, as well as the tensile strength of Tecoflex VGs, are studied. Solutions of Tecoflex or Pellethane with gelatin and bivalirudin in 1,1,1,3,3,3-hexafluoroisopropanol are used for VG production. After 1, 12, and 24 weeks of VG implantation in the infrarenal position of the abdominal aorta ofWistar rats, VGs are explanted, fixed in formalin, freed from outer tissues, dialyzed, and dried. The polyurethanes are extracted from VGs by dispersion/extraction in tetrahydrofuran (THF) and freed from the excess of THF-insoluble biopolymers. The stability of polyurethanes is assessed by IR spectroscopy and gel permeation chromatography. Pellethane has emerged to be stable at all experimental points. Tecoflex loses approximately 10% of its molecular weight (both Mn and Mw) after 3 months and restored its initial value within 6 months of its functioning as a graft. Mechanical testing demonstrates a 30% reduction in the tensile strength after 3 months in VG and a 10% increase after 6 months. The stability and mechanical properties of polyurethane-based VGs demonstrate their utility for the reconstitution of damaged arteries.

AB - The biostability of the polyurethanes Tecoflex EG-80A and Pellethane 2363-80A, used as basic polymers of the vascular grafts (VGs) produced by electrospinning, as well as the tensile strength of Tecoflex VGs, are studied. Solutions of Tecoflex or Pellethane with gelatin and bivalirudin in 1,1,1,3,3,3-hexafluoroisopropanol are used for VG production. After 1, 12, and 24 weeks of VG implantation in the infrarenal position of the abdominal aorta ofWistar rats, VGs are explanted, fixed in formalin, freed from outer tissues, dialyzed, and dried. The polyurethanes are extracted from VGs by dispersion/extraction in tetrahydrofuran (THF) and freed from the excess of THF-insoluble biopolymers. The stability of polyurethanes is assessed by IR spectroscopy and gel permeation chromatography. Pellethane has emerged to be stable at all experimental points. Tecoflex loses approximately 10% of its molecular weight (both Mn and Mw) after 3 months and restored its initial value within 6 months of its functioning as a graft. Mechanical testing demonstrates a 30% reduction in the tensile strength after 3 months in VG and a 10% increase after 6 months. The stability and mechanical properties of polyurethane-based VGs demonstrate their utility for the reconstitution of damaged arteries.

KW - Electrospinning

KW - Gelatin

KW - Pellethane

KW - Polyurethane stability in vivo

KW - Tecoflex

KW - Vascular grafts

KW - BIOCOMPATIBILITY

KW - vascular grafts

KW - polyurethane stability in vivo

KW - tecoflex

KW - electrospinning

KW - FABRICATION

KW - pellethane

KW - ALBUMIN

KW - SCAFFOLDS

KW - BIOSTABILITY

KW - NEW-GENERATION

KW - gelatin

UR - http://www.scopus.com/inward/record.url?scp=85086627614&partnerID=8YFLogxK

U2 - 10.3390/POLYM12040845

DO - 10.3390/POLYM12040845

M3 - Article

C2 - 32272564

AN - SCOPUS:85086627614

VL - 12

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 4

M1 - 845

ER -