In situ functionalization of HPLC monolithic columns based on divinylbenzene-styrene-4-vinylbenzyl chloride

Standard

In situ functionalization of HPLC monolithic columns based on divinylbenzene-styrene-4-vinylbenzyl chloride. / Sotnikova, Yulia S.; Patrushev, Yuri V.; Sidelnikov, Vladimir N. et al.

In: Talanta, Vol. 220, 121400, 01.12.2020.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{97b387cb50f7435ab00d3ab0f0f7ea7c,

title = "In situ functionalization of HPLC monolithic columns based on divinylbenzene-styrene-4-vinylbenzyl chloride",

abstract = "The properties of chromatographic columns are largely determined by functional groups located on the sorbent surface. For monolithic columns, surface functional groups can be created during synthesis stage or by chemical bonding with the complete surface of the sorbent. One of sorbent modification approach is to use on-column click reactions with surface reactive groups. In this study, the surface treatment of monolithic sorbent based on divinylbenzene (DVB), styrene (St) and 4-vinylbenzyl chloride (4VBC) copolymer by heterocyclic nitrogen-containing compounds 1-methylimidazole (1MI), 2-methylimidazole (2MI), 2-methylpyridine (2 MP) and 4-methylpyridine (4 MP) is described. The reaction of nitrogen-containing heterocycles with chloromethyl fragments on the surface results in formation of ion pairs and significantly changes the selectivity of monolithic columns. The chromatographic properties of prepared columns are studied. Modified columns can be operated in reversed-phase (RP) chromatography or in hydrophilic interaction liquid chromatography (HILIC) with different composition of the mobile phase. Separation examples of various chemical substances classes are given.",

keywords = "Click reactions, HPLC, Mixed-mode chromatography, Monolithic columns, Porous polymer, SEPARATION, CHROMATOGRAPHY, PROTEINS",

author = "Sotnikova, {Yulia S.} and Patrushev, {Yuri V.} and Sidelnikov, {Vladimir N.} and Mazaeva, {Anastasia A.}",

year = "2020",

month = dec,

day = "1",

doi = "10.1016/j.talanta.2020.121400",

language = "English",

volume = "220",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - In situ functionalization of HPLC monolithic columns based on divinylbenzene-styrene-4-vinylbenzyl chloride

AU - Sotnikova, Yulia S.

AU - Patrushev, Yuri V.

AU - Sidelnikov, Vladimir N.

AU - Mazaeva, Anastasia A.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - The properties of chromatographic columns are largely determined by functional groups located on the sorbent surface. For monolithic columns, surface functional groups can be created during synthesis stage or by chemical bonding with the complete surface of the sorbent. One of sorbent modification approach is to use on-column click reactions with surface reactive groups. In this study, the surface treatment of monolithic sorbent based on divinylbenzene (DVB), styrene (St) and 4-vinylbenzyl chloride (4VBC) copolymer by heterocyclic nitrogen-containing compounds 1-methylimidazole (1MI), 2-methylimidazole (2MI), 2-methylpyridine (2 MP) and 4-methylpyridine (4 MP) is described. The reaction of nitrogen-containing heterocycles with chloromethyl fragments on the surface results in formation of ion pairs and significantly changes the selectivity of monolithic columns. The chromatographic properties of prepared columns are studied. Modified columns can be operated in reversed-phase (RP) chromatography or in hydrophilic interaction liquid chromatography (HILIC) with different composition of the mobile phase. Separation examples of various chemical substances classes are given.

AB - The properties of chromatographic columns are largely determined by functional groups located on the sorbent surface. For monolithic columns, surface functional groups can be created during synthesis stage or by chemical bonding with the complete surface of the sorbent. One of sorbent modification approach is to use on-column click reactions with surface reactive groups. In this study, the surface treatment of monolithic sorbent based on divinylbenzene (DVB), styrene (St) and 4-vinylbenzyl chloride (4VBC) copolymer by heterocyclic nitrogen-containing compounds 1-methylimidazole (1MI), 2-methylimidazole (2MI), 2-methylpyridine (2 MP) and 4-methylpyridine (4 MP) is described. The reaction of nitrogen-containing heterocycles with chloromethyl fragments on the surface results in formation of ion pairs and significantly changes the selectivity of monolithic columns. The chromatographic properties of prepared columns are studied. Modified columns can be operated in reversed-phase (RP) chromatography or in hydrophilic interaction liquid chromatography (HILIC) with different composition of the mobile phase. Separation examples of various chemical substances classes are given.

KW - Click reactions

KW - HPLC

KW - Mixed-mode chromatography

KW - Monolithic columns

KW - Porous polymer

KW - SEPARATION

KW - CHROMATOGRAPHY

KW - PROTEINS

UR - http://www.scopus.com/inward/record.url?scp=85087968588&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2020.121400

DO - 10.1016/j.talanta.2020.121400

M3 - Article

C2 - 32928418

AN - SCOPUS:85087968588

VL - 220

JO - Talanta

JF - Talanta

SN - 0039-9140

M1 - 121400

ER -

ID: 24768847