Research output: Contribution to journal › Article › peer-review
In Situ Enhanced Yields of Microbial Nanowires: The Key Role of Environmental Stress. / Song, Bo; Wang, Zhibin; Wang, Lei et al.
In: ACS Biomaterials Science & Engineering, Vol. 9, No. 6, 12.06.2023, p. 3253-3261.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - In Situ Enhanced Yields of Microbial Nanowires: The Key Role of Environmental Stress
AU - Song, Bo
AU - Wang, Zhibin
AU - Wang, Lei
AU - Wang, Qi
AU - Li, Jiaxin
AU - Song, Manjiao
AU - Ali, Jafar
AU - Wang, Yaxin
AU - Glebov, Evgeni M
AU - Zhuang, Xuliang
N1 - This research was funded by the National Natural Science Foundation of China (21976197, 42230411, and 42177099) and the Fundamental Research Funds for the Central Universities (E1E40508X2).
PY - 2023/6/12
Y1 - 2023/6/12
N2 - The conductive microbial nanowires of Geobacter sulfurreducens serve as a model for long-range extracellular electron transfer (EET), which is considered a revolutionary "green" nanomaterial in the fields of bioelectronics, renewable energy, and bioremediation. However, there is no efficient pathway to induce microorganisms to express a large amount of microbial nanowires. Here, several strategies have been used to successfully induce the expression of microbial nanowires. Microbial nanowire expression was closely related to the concentration of electron acceptors. The microbial nanowire was around 17.02 μm in length, more than 3 times compared to its own length. The graphite electrode was used as an alternative electron acceptor by G. sulfurreducens, which obtained a fast start-up time of 44 h in microbial fuel cells (MFCs). Meanwhile, Fe(III) citrate-coated sugarcane carbon and biochar were prepared to test the applicability of these strategies in the actual microbial community. The unsatisfied EET efficiency between c-type cytochrome and extracellular insoluble electron receptors promoted the expression of microbial nanowires. Hence, microbial nanowires were proposed to be an effective survival strategy for G. sulfurreducens to cope with various environmental stresses. Based on this top-down strategy of artificially constructed microbial environmental stress, this study is of great significance for exploring more efficient methods to induce microbial nanowires expression.
AB - The conductive microbial nanowires of Geobacter sulfurreducens serve as a model for long-range extracellular electron transfer (EET), which is considered a revolutionary "green" nanomaterial in the fields of bioelectronics, renewable energy, and bioremediation. However, there is no efficient pathway to induce microorganisms to express a large amount of microbial nanowires. Here, several strategies have been used to successfully induce the expression of microbial nanowires. Microbial nanowire expression was closely related to the concentration of electron acceptors. The microbial nanowire was around 17.02 μm in length, more than 3 times compared to its own length. The graphite electrode was used as an alternative electron acceptor by G. sulfurreducens, which obtained a fast start-up time of 44 h in microbial fuel cells (MFCs). Meanwhile, Fe(III) citrate-coated sugarcane carbon and biochar were prepared to test the applicability of these strategies in the actual microbial community. The unsatisfied EET efficiency between c-type cytochrome and extracellular insoluble electron receptors promoted the expression of microbial nanowires. Hence, microbial nanowires were proposed to be an effective survival strategy for G. sulfurreducens to cope with various environmental stresses. Based on this top-down strategy of artificially constructed microbial environmental stress, this study is of great significance for exploring more efficient methods to induce microbial nanowires expression.
KW - electron acceptors
KW - environmental stresses
KW - extracellular electron transfer
KW - microbial nanowires
KW - nanomaterial
KW - Electron Transport
KW - Ferric Compounds/metabolism
KW - Bioelectric Energy Sources
KW - Electric Conductivity
KW - Nanowires
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85159597764&origin=inward&txGid=0eea7f38114825510eee623dd6fb6e9b
UR - https://www.mendeley.com/catalogue/a31d879e-6cfd-3024-90d2-158042462c33/
U2 - 10.1021/acsbiomaterials.3c00313
DO - 10.1021/acsbiomaterials.3c00313
M3 - Article
C2 - 37146257
VL - 9
SP - 3253
EP - 3261
JO - ACS Biomaterials Science & Engineering
JF - ACS Biomaterials Science & Engineering
SN - 2373-9878
IS - 6
ER -
ID: 49443039