Research output: Contribution to journal › Article › peer-review
In Silico Transcriptomic Analysis of Wound-Healing-Associated Genes in Malignant Pleural Mesothelioma. / Rouka, Erasmia; Beltsios, Eleftherios; Goundaroulis, Dimos et al.
In: Medicina (Kaunas, Lithuania), Vol. 55, No. 6, 267, 12.06.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - In Silico Transcriptomic Analysis of Wound-Healing-Associated Genes in Malignant Pleural Mesothelioma
AU - Rouka, Erasmia
AU - Beltsios, Eleftherios
AU - Goundaroulis, Dimos
AU - Vavougios, Georgios D.
AU - Solenov, Evgeniy I.
AU - Hatzoglou, Chrissi
AU - Gourgoulianis, Konstantinos I.
AU - Zarogiannis, Sotirios G.
PY - 2019/6/12
Y1 - 2019/6/12
N2 - Background and objectives: Malignant pleural mesothelioma (MPM) is a devastating malignancy with poor prognosis. Reliable biomarkers for MPM diagnosis, monitoring, and prognosis are needed. The aim of this study was to identify genes associated with wound healing processes whose expression could serve as a prognostic factor in MPM patients. Materials and Methods: We used data mining techniques and transcriptomic analysis so as to assess the differential transcriptional expression of wound-healing-associated genes in MPM. Moreover, we investigated the potential prognostic value as well as the functional enrichments of gene ontologies relative to microRNAs (miRNAs) of the significantly differentially expressed wound-healing-related genes in MPM. Results: Out of the 82 wound-healing-associated genes analyzed, 30 were found significantly deregulated in MPM. Kaplan-Meier analysis revealed that low ITGAV gene expression could serve as a prognostic factor favoring survival of MPM patients. Finally, gene ontology annotation enrichment analysis pointed to the members of the hsa-miR-143, hsa-miR-223, and the hsa-miR-29 miRNA family members as important regulators of the deregulated wound healing genes. Conclusions: 30 wound-healing-related genes were significantly deregulated in MPM, which are potential targets of hsa-miR-143, hsa-miR-223, and the hsa-miR-29 miRNA family members. Out of those genes, ITGAV gene expression was a prognostic factor of overall survival in MPM. Our results highlight the role of impaired tissue repair in MPM development and should be further validated experimentally.
AB - Background and objectives: Malignant pleural mesothelioma (MPM) is a devastating malignancy with poor prognosis. Reliable biomarkers for MPM diagnosis, monitoring, and prognosis are needed. The aim of this study was to identify genes associated with wound healing processes whose expression could serve as a prognostic factor in MPM patients. Materials and Methods: We used data mining techniques and transcriptomic analysis so as to assess the differential transcriptional expression of wound-healing-associated genes in MPM. Moreover, we investigated the potential prognostic value as well as the functional enrichments of gene ontologies relative to microRNAs (miRNAs) of the significantly differentially expressed wound-healing-related genes in MPM. Results: Out of the 82 wound-healing-associated genes analyzed, 30 were found significantly deregulated in MPM. Kaplan-Meier analysis revealed that low ITGAV gene expression could serve as a prognostic factor favoring survival of MPM patients. Finally, gene ontology annotation enrichment analysis pointed to the members of the hsa-miR-143, hsa-miR-223, and the hsa-miR-29 miRNA family members as important regulators of the deregulated wound healing genes. Conclusions: 30 wound-healing-related genes were significantly deregulated in MPM, which are potential targets of hsa-miR-143, hsa-miR-223, and the hsa-miR-29 miRNA family members. Out of those genes, ITGAV gene expression was a prognostic factor of overall survival in MPM. Our results highlight the role of impaired tissue repair in MPM development and should be further validated experimentally.
KW - in silico
KW - malignant pleural mesothelioma
KW - miRNA
KW - transcriptomics
KW - wound healing
KW - Wound healing
KW - Transcriptomics
KW - In silico
KW - Malignant pleural mesothelioma
KW - SURVIVAL
KW - METASTASIS
KW - TUMOR SUPPRESSORS
KW - BIOMARKERS
KW - EPITHELIAL-MESENCHYMAL TRANSITION
KW - CARCINOGENESIS
KW - INFLAMMATION
KW - EXTRACELLULAR-MATRIX
KW - EXPRESSION
KW - ASBESTOS
UR - http://www.scopus.com/inward/record.url?scp=85068447444&partnerID=8YFLogxK
U2 - 10.3390/medicina55060267
DO - 10.3390/medicina55060267
M3 - Article
C2 - 31212858
AN - SCOPUS:85068447444
VL - 55
JO - Medicina
JF - Medicina
SN - 1010-660X
IS - 6
M1 - 267
ER -
ID: 20778163