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Identification of the xenograft and its ascendant sphere-forming cell line as belonging to EBV-induced lymphoma, and characterization of the status of sphere-forming cells. / Dolgova, Evgeniya V.; Petrova, Daria D.; Proskurina, Anastasia S. et al.
In: Cancer Cell International, Vol. 19, No. 1, 120, 06.05.2019, p. 120.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Identification of the xenograft and its ascendant sphere-forming cell line as belonging to EBV-induced lymphoma, and characterization of the status of sphere-forming cells
AU - Dolgova, Evgeniya V.
AU - Petrova, Daria D.
AU - Proskurina, Anastasia S.
AU - Ritter, Genrikh S.
AU - Kisaretova, Polina E.
AU - Potter, Ekaterina A.
AU - Efremov, Yaroslav R.
AU - Bayborodin, Sergey I.
AU - Karamysheva, Tatiana V.
AU - Romanenko, Margarita V.
AU - Netesov, Sergey V.
AU - Taranov, Oleg S.
AU - Ostanin, Aleksandr A.
AU - Chernykh, Elena R.
AU - Bogachev, Sergey S.
PY - 2019/5/6
Y1 - 2019/5/6
N2 - Background: We have characterized the human cell line arised from the Epstein-Barr virus (EBV) positive multiple myeloma aspirate subjected to the long-term cultivation. This cell line has acquired the ability to form free-floating spheres and to produce a xenograft upon transplantation into NOD/SCID mice. Methods: Cells from both in vitro culture and developed xenografts were investigated with a number of analytical approaches, including pathomorphological analysis, FISH analysis, and analysis of the surface antigens and of the VDJ locus rearrangement. Results: The obtained results, as well as the confirmed presence of EBV, testify that both biological systems are derived from B-cells, which, in turn, is a progeny of the EBV-transformed B-cellular clone that supplanted the primordial multiple myeloma cells. Next we assessed whether cells that (i) were constantly present in vitro in the investigated cell line, (ii) were among the sphere-forming cells, and (iii) were capable of internalizing a fluorescent TAMRA-labeled DNA probe (TAMRA+ cells) belonged to one of the three types of undifferentiated bone marrow cells of a multiple myeloma patient: CD34+ hematopoietic stem cells, CD90+ mesenchymal stem cells, and clonotypic multiple myeloma cell. Conclusion: TAMRA+ cells were shown to constitute the fourth independent subpopulation of undifferentiated bone marrow cells of the multiple myeloma patient. We have demonstrated the formation of ectopic contacts between TAMRA+ cells and cells of other types in culture, in particular with CD90+ mesenchymal stem cells, followed by the transfer of some TAMRA+ cell material into the contacted cell.
AB - Background: We have characterized the human cell line arised from the Epstein-Barr virus (EBV) positive multiple myeloma aspirate subjected to the long-term cultivation. This cell line has acquired the ability to form free-floating spheres and to produce a xenograft upon transplantation into NOD/SCID mice. Methods: Cells from both in vitro culture and developed xenografts were investigated with a number of analytical approaches, including pathomorphological analysis, FISH analysis, and analysis of the surface antigens and of the VDJ locus rearrangement. Results: The obtained results, as well as the confirmed presence of EBV, testify that both biological systems are derived from B-cells, which, in turn, is a progeny of the EBV-transformed B-cellular clone that supplanted the primordial multiple myeloma cells. Next we assessed whether cells that (i) were constantly present in vitro in the investigated cell line, (ii) were among the sphere-forming cells, and (iii) were capable of internalizing a fluorescent TAMRA-labeled DNA probe (TAMRA+ cells) belonged to one of the three types of undifferentiated bone marrow cells of a multiple myeloma patient: CD34+ hematopoietic stem cells, CD90+ mesenchymal stem cells, and clonotypic multiple myeloma cell. Conclusion: TAMRA+ cells were shown to constitute the fourth independent subpopulation of undifferentiated bone marrow cells of the multiple myeloma patient. We have demonstrated the formation of ectopic contacts between TAMRA+ cells and cells of other types in culture, in particular with CD90+ mesenchymal stem cells, followed by the transfer of some TAMRA+ cell material into the contacted cell.
KW - B-cell lymphoma
KW - Clonotypic B cell
KW - Lymphoblastoid cell line
KW - Mesenchymal stem cells
KW - TAMRA-labeled DNA probe
KW - CANCER-CELLS
KW - MULTIPLE-MYELOMA
KW - DEEP-SEQUENCING METHOD
KW - FUSION
KW - ACUTE MYELOID-LEUKEMIA
KW - PROGNOSTIC VALUE
KW - MESENCHYMAL STEM-CELLS
KW - IN-VITRO
KW - DISEASE
KW - EXPRESSION
UR - http://www.scopus.com/inward/record.url?scp=85068346632&partnerID=8YFLogxK
U2 - 10.1186/s12935-019-0842-x
DO - 10.1186/s12935-019-0842-x
M3 - Article
C2 - 31080361
AN - SCOPUS:85068346632
VL - 19
SP - 120
JO - Cancer Cell International
JF - Cancer Cell International
SN - 1475-2867
IS - 1
M1 - 120
ER -
ID: 20778373