Research output: Contribution to journal › Article › peer-review
Human Gut Microbiome Response to Short-Term Bifidobacterium-Based Probiotic Treatment. / Naumova, Natalia; Alikina, Tatiana; Tupikin, Alexey et al.
In: Indian Journal of Microbiology, Vol. 60, No. 4, 01.12.2020, p. 451-457.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Human Gut Microbiome Response to Short-Term Bifidobacterium-Based Probiotic Treatment
AU - Naumova, Natalia
AU - Alikina, Tatiana
AU - Tupikin, Alexey
AU - Kalmykova, Anna
AU - Soldatova, Galina
AU - Vlassov, Valentin
AU - Kabilov, Marsel
N1 - © Association of Microbiologists of India 2020.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Gut microbiota is believed to play a crucial role in modulating obesity in humans, and probiotics affecting gut microbiota can alleviate some of the obesity-related health complications. The study was aimed to investigate changes in the composition of the gut microbiome in obese humans due to short-term (2 weeks) treatment of obese patients with a probiotic preparation containing Bifidobacterium longum. Faecal microbiome diversity was studied using the 16S amplicon sequencing by Illumina MiSeq. Bioinformatic analysis showed distribution across 14 phyla (with Firmicutes and Bacteroidetes dominating), 21 class, 125 genera and 973 OTUs. The probiotic treatment decreased relative abundance of Bacteroidetes (Prevotellaceae and Bacteroidaceae), while increasing that of Actinobacteria (Bifidobacteriaceae and Coriobacteriaceae), and Firmicutes (Negativicutes: Veillonellaceae and Clostridia: Peptostreptococcaceae). The probiotic treatment decreased total blood sugar and increased patients’ assessment of their physical and mental health. Thus even the short-term Bifidobacterium-based probiotic treatment brought significant compositional changes in the 16S rRNA gene diversity in faecal bacterial assemblages by increasing beneficial and decreasing pathogenic or opportunistic bacteria; the related shifts in life quality assessment necessitate further research into the causal relationships involved.
AB - Gut microbiota is believed to play a crucial role in modulating obesity in humans, and probiotics affecting gut microbiota can alleviate some of the obesity-related health complications. The study was aimed to investigate changes in the composition of the gut microbiome in obese humans due to short-term (2 weeks) treatment of obese patients with a probiotic preparation containing Bifidobacterium longum. Faecal microbiome diversity was studied using the 16S amplicon sequencing by Illumina MiSeq. Bioinformatic analysis showed distribution across 14 phyla (with Firmicutes and Bacteroidetes dominating), 21 class, 125 genera and 973 OTUs. The probiotic treatment decreased relative abundance of Bacteroidetes (Prevotellaceae and Bacteroidaceae), while increasing that of Actinobacteria (Bifidobacteriaceae and Coriobacteriaceae), and Firmicutes (Negativicutes: Veillonellaceae and Clostridia: Peptostreptococcaceae). The probiotic treatment decreased total blood sugar and increased patients’ assessment of their physical and mental health. Thus even the short-term Bifidobacterium-based probiotic treatment brought significant compositional changes in the 16S rRNA gene diversity in faecal bacterial assemblages by increasing beneficial and decreasing pathogenic or opportunistic bacteria; the related shifts in life quality assessment necessitate further research into the causal relationships involved.
KW - 16S rRNA gene sequencing
KW - Gut microbiome
KW - Humans
KW - Obesity
KW - Probiotic
KW - SPORTS
KW - FECAL MICROBIOTA
UR - http://www.scopus.com/inward/record.url?scp=85085376073&partnerID=8YFLogxK
U2 - 10.1007/s12088-020-00888-1
DO - 10.1007/s12088-020-00888-1
M3 - Article
C2 - 33087994
AN - SCOPUS:85085376073
VL - 60
SP - 451
EP - 457
JO - Indian Journal of Microbiology
JF - Indian Journal of Microbiology
SN - 0046-8991
IS - 4
ER -
ID: 24394785