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Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. / Hillman, Courtney; Fontana, Barbara D.; Amstislavskaya, Tamara G. et al.

In: Lab Animal, Vol. 54, No. 6, 15081, 27.05.2025, p. 156-164.

Research output: Contribution to journal › Article › peer-review

Harvard

Hillman, C, Fontana, BD, Amstislavskaya, TG, Gorbunova, MA, Altenhofen, S, Barthelson, K, Bastos, LM, Borba, JV, Bonan, CD, Brennan, CH, Farías-Cea, A, Cooper, A, Corcoran, J, Dondossola, ER, Martinez-Duran, LM, Gallas-Lopes, M, Galstyan, DS, Garcia, EO, Gerken, E, Hindges, R, Kenney, JW, Kleshchev, MA, Kolesnikova, TO, Leggieri, A, Khatsko, SL, Lardelli, M, Lodetti, G, Lombardelli, G, Luchiari, AC, Portela, SM, Medan, V, Moutinho, LM, Nekhoroshev, EV, Petersen, BD, Petrunich-Rutherford, ML, Piato, A, Porfiri, M, Read, E, Resmim, CM, Rico, EP, Rosemberg, DB, de Abreu, MS, Salazar, CA, Stahlhofer-Buss, T, Teixeira, JR, Valentim, AM, Zhdanov, AV, Iturriaga-Vásquez, P, Wang, X, Wong, RY, Kalueff, AV & Parker, MO 2025, 'Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study', Lab Animal, vol. 54, no. 6, 15081, pp. 156-164. https://doi.org/10.1038/s41684-025-01548-x

APA

Hillman, C., Fontana, B. D., Amstislavskaya, T. G., Gorbunova, M. A., Altenhofen, S., Barthelson, K., Bastos, L. M., Borba, J. V., Bonan, C. D., Brennan, C. H., Farías-Cea, A., Cooper, A., Corcoran, J., Dondossola, E. R., Martinez-Duran, L. M., Gallas-Lopes, M., Galstyan, D. S., Garcia, E. O., Gerken, E., ... Parker, M. O. (2025). Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. Lab Animal, 54(6), 156-164. [15081]. https://doi.org/10.1038/s41684-025-01548-x

Vancouver

Hillman C, Fontana BD, Amstislavskaya TG, Gorbunova MA, Altenhofen S, Barthelson K et al. Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. Lab Animal. 2025 May 27;54(6):156-164. 15081. doi: 10.1038/s41684-025-01548-x

Author

Hillman, Courtney ; Fontana, Barbara D. ; Amstislavskaya, Tamara G. et al. / Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study. In: Lab Animal. 2025 ; Vol. 54, No. 6. pp. 156-164.

BibTeX

@article{10a22e7d8ea74f77a2a87b354f0b88a8,
title = "Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study",
abstract = "The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.",
author = "Courtney Hillman and Fontana, {Barbara D.} and Amstislavskaya, {Tamara G.} and Gorbunova, {Maria A.} and Stefani Altenhofen and Karissa Barthelson and Bastos, {Leonardo M.} and Borba, {Jo{\~a}o V.} and Bonan, {Carla D.} and Brennan, {Caroline H.} and Amaury Far{\'i}as-Cea and Austin Cooper and Jamie Corcoran and Dondossola, {Eduardo R.} and Martinez-Duran, {Luis M.} and Matheus Gallas-Lopes and Galstyan, {David S.} and Garcia, {Ella O.} and Ewan Gerken and Robert Hindges and Kenney, {Justin W.} and Kleshchev, {Maxim A.} and Kolesnikova, {Tatiana O.} and Adele Leggieri and Khatsko, {Sergey L.} and Michael Lardelli and Guilherme Lodetti and Giulia Lombardelli and Luchiari, {Ana C.} and Portela, {Stefani M.} and Violeta Medan and Moutinho, {Lirane M.} and Nekhoroshev, {Evgeny V.} and Petersen, {Barbara D.} and Petrunich-Rutherford, {Maureen L.} and Angelo Piato and Maurizio Porfiri and Emily Read and Resmim, {C{\'a}ssio M.} and Rico, {Eduardo P.} and Rosemberg, {Denis B.} and {de Abreu}, {Murilo S.} and Salazar, {Catia A.} and Thailana Stahlhofer-Buss and Teixeira, {J{\'u}lia R.} and Valentim, {Ana M.} and Zhdanov, {Alexander V.} and Patricio Iturriaga-V{\'a}squez and Xian Wang and Wong, {Ryan Y.} and Kalueff, {Allan V.} and Parker, {Matthew O.}",
note = "We thank the technical teams and animal care and use staff for assistance in fish husbandry at all institutions. C.H. was funded by a studentship from DSTL (UK). M.O.P. received funding from NC3Rs (UK). D.S.G. was supported by St. Petersburg State University fund project ID 95443748 (Russia). D.B.R., C.D.B., B.D.P. and A.C.L. were supported by the Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq) fellowship grant (DBR process number 307690/2021-0, CDB process number 306115/2023-9, BDP process number 140312/2021-8 and ACL process number 308245/2023-7). S.A. was supported by Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior (CAPES; finance code 001). R.Y.W. was supported by the National Institutes of Health (R15MH113074 & 5P20GM103427). A.V.K. was supported by the School of Science, Xi\u2019an Jiaotong-Liverpool University (XJTLU, China). K.B. is supported by a Race Against Dementia \u2013 Dementia Australia Research Foundation Postdoctoral Fellowship. V.M. was supported by Agencia Nacional de Promoci\u00F3n Cient\u00EDfica y Tecnol\u00F3gica (ANPCyT-PICT2020-2137). G. Lombardelli and M.P. were supported by the National Science Foundation under grant no. CMMI 1901697. J.W.K. and B.D.F. were supported by the National Institutes of Health (R35GM142566). A.P. and C.D.B. were supported by the Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq, A.P. process number 305968/2023-8 and C.D.B. process number 402097/2023-8). C.H.B. and A.L. were supported by the NIH grant U01 DA044400-03. A.M.V. was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia (FCT; Portugal) through a Norma Transit\u00F3ria contract with i3S. E.O.G. and C.A.S. were supported by the National Science Foundation, award number EES 2308500, 2023-2028. E.R. was supported by a PhD studentship from the MRC Centre for Neurodevelopmental Disorders [MR/W006251/1]. P.I.-V. was supported by FONDECYT Grant 11250688. T.O.K. was supported by Sirius University of Science and Technology (Sochi, Russia, project ID NRB-RND-2116). Maurizio Porfiri lab are thankful to Tianjun Han for his help in setting up the automated tracking system.",
year = "2025",
month = may,
day = "27",
doi = "10.1038/s41684-025-01548-x",
language = "русский",
volume = "54",
pages = "156--164",
journal = "Lab Animal",
issn = "0093-7355",
publisher = "Nature Publishing Group",
number = "6",

}

RIS

TY - JOUR

T1 - Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study

AU - Hillman, Courtney

AU - Fontana, Barbara D.

AU - Amstislavskaya, Tamara G.

AU - Gorbunova, Maria A.

AU - Altenhofen, Stefani

AU - Barthelson, Karissa

AU - Bastos, Leonardo M.

AU - Borba, João V.

AU - Bonan, Carla D.

AU - Brennan, Caroline H.

AU - Farías-Cea, Amaury

AU - Cooper, Austin

AU - Corcoran, Jamie

AU - Dondossola, Eduardo R.

AU - Martinez-Duran, Luis M.

AU - Gallas-Lopes, Matheus

AU - Galstyan, David S.

AU - Garcia, Ella O.

AU - Gerken, Ewan

AU - Hindges, Robert

AU - Kenney, Justin W.

AU - Kleshchev, Maxim A.

AU - Kolesnikova, Tatiana O.

AU - Leggieri, Adele

AU - Khatsko, Sergey L.

AU - Lardelli, Michael

AU - Lodetti, Guilherme

AU - Lombardelli, Giulia

AU - Luchiari, Ana C.

AU - Portela, Stefani M.

AU - Medan, Violeta

AU - Moutinho, Lirane M.

AU - Nekhoroshev, Evgeny V.

AU - Petersen, Barbara D.

AU - Petrunich-Rutherford, Maureen L.

AU - Piato, Angelo

AU - Porfiri, Maurizio

AU - Read, Emily

AU - Resmim, Cássio M.

AU - Rico, Eduardo P.

AU - Rosemberg, Denis B.

AU - de Abreu, Murilo S.

AU - Salazar, Catia A.

AU - Stahlhofer-Buss, Thailana

AU - Teixeira, Júlia R.

AU - Valentim, Ana M.

AU - Zhdanov, Alexander V.

AU - Iturriaga-Vásquez, Patricio

AU - Wang, Xian

AU - Wong, Ryan Y.

AU - Kalueff, Allan V.

AU - Parker, Matthew O.

N1 - We thank the technical teams and animal care and use staff for assistance in fish husbandry at all institutions. C.H. was funded by a studentship from DSTL (UK). M.O.P. received funding from NC3Rs (UK). D.S.G. was supported by St. Petersburg State University fund project ID 95443748 (Russia). D.B.R., C.D.B., B.D.P. and A.C.L. were supported by the Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq) fellowship grant (DBR process number 307690/2021-0, CDB process number 306115/2023-9, BDP process number 140312/2021-8 and ACL process number 308245/2023-7). S.A. was supported by Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior (CAPES; finance code 001). R.Y.W. was supported by the National Institutes of Health (R15MH113074 & 5P20GM103427). A.V.K. was supported by the School of Science, Xi\u2019an Jiaotong-Liverpool University (XJTLU, China). K.B. is supported by a Race Against Dementia \u2013 Dementia Australia Research Foundation Postdoctoral Fellowship. V.M. was supported by Agencia Nacional de Promoci\u00F3n Cient\u00EDfica y Tecnol\u00F3gica (ANPCyT-PICT2020-2137). G. Lombardelli and M.P. were supported by the National Science Foundation under grant no. CMMI 1901697. J.W.K. and B.D.F. were supported by the National Institutes of Health (R35GM142566). A.P. and C.D.B. were supported by the Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq, A.P. process number 305968/2023-8 and C.D.B. process number 402097/2023-8). C.H.B. and A.L. were supported by the NIH grant U01 DA044400-03. A.M.V. was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia (FCT; Portugal) through a Norma Transit\u00F3ria contract with i3S. E.O.G. and C.A.S. were supported by the National Science Foundation, award number EES 2308500, 2023-2028. E.R. was supported by a PhD studentship from the MRC Centre for Neurodevelopmental Disorders [MR/W006251/1]. P.I.-V. was supported by FONDECYT Grant 11250688. T.O.K. was supported by Sirius University of Science and Technology (Sochi, Russia, project ID NRB-RND-2116). Maurizio Porfiri lab are thankful to Tianjun Han for his help in setting up the automated tracking system.

PY - 2025/5/27

Y1 - 2025/5/27

N2 - The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.

AB - The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes. Global collaborative studies offer a unique solution to this problem. Here, we present a global collaboration using the zebrafish (Danio rerio) novel tank test, a popular behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show that female zebrafish exhibit more anxiety-like behavior than males, highlighting sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, also influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (for example, rotifers) mitigated anxiety-like behaviors. Environmental stressors, such as noise and transportation, significantly impacted behavior. We recommend standardizing testing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds and minimizing stressors to improve the reliability of zebrafish behavioral studies.

UR - https://www.mendeley.com/catalogue/2bd6a087-94e8-3cf4-a522-c99c2ec79bb0/

UR - https://pubmed.ncbi.nlm.nih.gov/40419676/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105006488764&origin=inward&txGid=d4546438cc9d6d845ade01d60a06c321

U2 - 10.1038/s41684-025-01548-x

DO - 10.1038/s41684-025-01548-x

M3 - статья

C2 - 40419676

VL - 54

SP - 156

EP - 164

JO - Lab Animal

JF - Lab Animal

SN - 0093-7355

IS - 6

M1 - 15081

ER -

ID: 67651231