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Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation. / Ushakov, Victor S.; Tsidulko, Alexandra Y.; De La Bourdonnaye, Gabin et al.

In: International Journal of Molecular Sciences, Vol. 18, No. 11, 2301, 01.11.2017.

Research output: Contribution to journalArticlepeer-review

Harvard

Ushakov, VS, Tsidulko, AY, De La Bourdonnaye, G, Kazanskaya, GM, Volkov, AM, Kiselev, RS, Kobozev, VV, Kostromskaya, DV, Gaytan, AS, Krivoshapkin, AL, Aidagulova, SV & Grigorieva, EV 2017, 'Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation', International Journal of Molecular Sciences, vol. 18, no. 11, 2301. https://doi.org/10.3390/ijms18112301

APA

Ushakov, V. S., Tsidulko, A. Y., De La Bourdonnaye, G., Kazanskaya, G. M., Volkov, A. M., Kiselev, R. S., Kobozev, V. V., Kostromskaya, D. V., Gaytan, A. S., Krivoshapkin, A. L., Aidagulova, S. V., & Grigorieva, E. V. (2017). Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation. International Journal of Molecular Sciences, 18(11), [2301]. https://doi.org/10.3390/ijms18112301

Vancouver

Ushakov VS, Tsidulko AY, De La Bourdonnaye G, Kazanskaya GM, Volkov AM, Kiselev RS et al. Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation. International Journal of Molecular Sciences. 2017 Nov 1;18(11):2301. doi: 10.3390/ijms18112301

Author

Ushakov, Victor S. ; Tsidulko, Alexandra Y. ; De La Bourdonnaye, Gabin et al. / Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 11.

BibTeX

@article{28f217739de448f9b7201552f4f57d13,
title = "Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation",
abstract = "Heparan sulfate (HS) is an important component of the extracellular matrix and cell surface, which plays a key role in cell-cell and cell-matrix interactions. Functional activity of HS directly depends on its structure, which determined by a complex system of HS biosynthetic enzymes. During malignant transformation, the system can undergo significant changes, but for glioma, HS biosynthesis has not been studied in detail. In this study, we performed a comparative analysis of the HS biosynthetic system in human gliomas of different grades. RT-PCR analysis showed that the overall transcriptional activity of the main HS biosynthesis-involved genes (EXT1, EXT2, NDST1, NDST2, GLCE, HS2ST1, HS3ST1, HS3ST2, HS6ST1, HS6ST2, SULF1, SULF2, HPSE) was decreased by 1.5-2-fold in Grade II-III glioma (p < 0.01) and by 3-fold in Grade IV glioma (glioblastoma multiforme, GBM) (p < 0.05), as compared with the para-tumourous tissue. The inhibition was mainly due to the elongation (a decrease in EXT1/2 expression by 3-4-fold) and 6-O-sulfation steps (a decrease in 6OST1/2 expression by 2-5-fold) of the HS biosynthesis. Heparanase (HPSE) expression was identified in 50% of GBM tumours by immunostaining, and was characterised by a high intratumoural heterogeneity of the presence of the HPSE protein. The detected disorganisation of the HS biosynthetic system in gliomas might be a potential molecular mechanism for the changes of HS structure and content in tumour microenvironments, contributing to the invasion of glioma cells and the development of the disease.",
keywords = "Biosynthesis, Extracellular matrix, Glioma, Heparan sulfate, Heparanase, Invasion, Sulfotransferase, Tumour microenvironment, PROTEOGLYCANS, heparanase, sulfotransferase, CANCER, heparan sulfate, invasion, ROLES, biosynthesis, GROWTH, glioma, tumour microenvironment, EXTRACELLULAR-MATRIX, EXPRESSION, extracellular matrix",
author = "Ushakov, {Victor S.} and Tsidulko, {Alexandra Y.} and {De La Bourdonnaye}, Gabin and Kazanskaya, {Galina M.} and Volkov, {Alexander M.} and Kiselev, {Roman S.} and Kobozev, {Vyacheslav V.} and Kostromskaya, {Diana V.} and Gaytan, {Alexey S.} and Krivoshapkin, {Alexei L.} and Aidagulova, {Svetlana V.} and Grigorieva, {Elvira V.}",
note = "Publisher Copyright: {\textcopyright} 2017 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2017",
month = nov,
day = "1",
doi = "10.3390/ijms18112301",
language = "English",
volume = "18",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "11",

}

RIS

TY - JOUR

T1 - Heparan sulfate biosynthetic system is inhibited in human glioma due to EXT1/2 and HS6ST1/2 down-regulation

AU - Ushakov, Victor S.

AU - Tsidulko, Alexandra Y.

AU - De La Bourdonnaye, Gabin

AU - Kazanskaya, Galina M.

AU - Volkov, Alexander M.

AU - Kiselev, Roman S.

AU - Kobozev, Vyacheslav V.

AU - Kostromskaya, Diana V.

AU - Gaytan, Alexey S.

AU - Krivoshapkin, Alexei L.

AU - Aidagulova, Svetlana V.

AU - Grigorieva, Elvira V.

N1 - Publisher Copyright: © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Heparan sulfate (HS) is an important component of the extracellular matrix and cell surface, which plays a key role in cell-cell and cell-matrix interactions. Functional activity of HS directly depends on its structure, which determined by a complex system of HS biosynthetic enzymes. During malignant transformation, the system can undergo significant changes, but for glioma, HS biosynthesis has not been studied in detail. In this study, we performed a comparative analysis of the HS biosynthetic system in human gliomas of different grades. RT-PCR analysis showed that the overall transcriptional activity of the main HS biosynthesis-involved genes (EXT1, EXT2, NDST1, NDST2, GLCE, HS2ST1, HS3ST1, HS3ST2, HS6ST1, HS6ST2, SULF1, SULF2, HPSE) was decreased by 1.5-2-fold in Grade II-III glioma (p < 0.01) and by 3-fold in Grade IV glioma (glioblastoma multiforme, GBM) (p < 0.05), as compared with the para-tumourous tissue. The inhibition was mainly due to the elongation (a decrease in EXT1/2 expression by 3-4-fold) and 6-O-sulfation steps (a decrease in 6OST1/2 expression by 2-5-fold) of the HS biosynthesis. Heparanase (HPSE) expression was identified in 50% of GBM tumours by immunostaining, and was characterised by a high intratumoural heterogeneity of the presence of the HPSE protein. The detected disorganisation of the HS biosynthetic system in gliomas might be a potential molecular mechanism for the changes of HS structure and content in tumour microenvironments, contributing to the invasion of glioma cells and the development of the disease.

AB - Heparan sulfate (HS) is an important component of the extracellular matrix and cell surface, which plays a key role in cell-cell and cell-matrix interactions. Functional activity of HS directly depends on its structure, which determined by a complex system of HS biosynthetic enzymes. During malignant transformation, the system can undergo significant changes, but for glioma, HS biosynthesis has not been studied in detail. In this study, we performed a comparative analysis of the HS biosynthetic system in human gliomas of different grades. RT-PCR analysis showed that the overall transcriptional activity of the main HS biosynthesis-involved genes (EXT1, EXT2, NDST1, NDST2, GLCE, HS2ST1, HS3ST1, HS3ST2, HS6ST1, HS6ST2, SULF1, SULF2, HPSE) was decreased by 1.5-2-fold in Grade II-III glioma (p < 0.01) and by 3-fold in Grade IV glioma (glioblastoma multiforme, GBM) (p < 0.05), as compared with the para-tumourous tissue. The inhibition was mainly due to the elongation (a decrease in EXT1/2 expression by 3-4-fold) and 6-O-sulfation steps (a decrease in 6OST1/2 expression by 2-5-fold) of the HS biosynthesis. Heparanase (HPSE) expression was identified in 50% of GBM tumours by immunostaining, and was characterised by a high intratumoural heterogeneity of the presence of the HPSE protein. The detected disorganisation of the HS biosynthetic system in gliomas might be a potential molecular mechanism for the changes of HS structure and content in tumour microenvironments, contributing to the invasion of glioma cells and the development of the disease.

KW - Biosynthesis

KW - Extracellular matrix

KW - Glioma

KW - Heparan sulfate

KW - Heparanase

KW - Invasion

KW - Sulfotransferase

KW - Tumour microenvironment

KW - PROTEOGLYCANS

KW - heparanase

KW - sulfotransferase

KW - CANCER

KW - heparan sulfate

KW - invasion

KW - ROLES

KW - biosynthesis

KW - GROWTH

KW - glioma

KW - tumour microenvironment

KW - EXTRACELLULAR-MATRIX

KW - EXPRESSION

KW - extracellular matrix

UR - http://www.scopus.com/inward/record.url?scp=85033573744&partnerID=8YFLogxK

U2 - 10.3390/ijms18112301

DO - 10.3390/ijms18112301

M3 - Article

C2 - 29104277

AN - SCOPUS:85033573744

VL - 18

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 11

M1 - 2301

ER -

ID: 9698556