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Glycosylase Base Editors : New Tools for Genome Editing. / Kulishova, Liliya M.; Zharkov, Dmitry O.

In: Biochemistry (Moscow), Vol. 91, No. 7, 31.07.2026, p. 1093-1113.

Research output: Contribution to journal › Review article › peer-review

Harvard

Kulishova, LM & Zharkov, DO 2026, 'Glycosylase Base Editors: New Tools for Genome Editing', Biochemistry (Moscow), vol. 91, no. 7, pp. 1093-1113. https://doi.org/10.1134/S0006297926601073

APA

Kulishova, L. M., & Zharkov, D. O. (2026). Glycosylase Base Editors: New Tools for Genome Editing. Biochemistry (Moscow), 91(7), 1093-1113. https://doi.org/10.1134/S0006297926601073

Vancouver

Kulishova LM, Zharkov DO. Glycosylase Base Editors: New Tools for Genome Editing. Biochemistry (Moscow). 2026 Jul 31;91(7):1093-1113. doi: 10.1134/S0006297926601073

Author

Kulishova, Liliya M. ; Zharkov, Dmitry O. / Glycosylase Base Editors : New Tools for Genome Editing. In: Biochemistry (Moscow). 2026 ; Vol. 91, No. 7. pp. 1093-1113.

BibTeX

@article{bd87f7822c4c44faa00533710f82ea41,
title = "Glycosylase Base Editors: New Tools for Genome Editing",
abstract = "Genome editing using the CRISPR/Cas9 system has become a staple of modern genome manipulation. In its original form, editing involved introducing double-strand breaks into DNA, which can cause genomic instability. The appearance of the first base editors in 2016 expanded the range of editing technologies and enabled single-nucleotide changes to be introduced into the genome through deamination of nucleobases, bypassing the double-strand break stage. Further development of base editors involves the incorporation of additional modules, DNA glycosylases, that can remove modified or even normal nucleobases and create non-instructive apurinic/apyrimidinic sites in DNA, significantly expanding the range of available single-nucleotide substitutions. This review examines the operating principles of the glycosylase base editors, the main limitations of these genome manipulation tools, and promising areas for the development of this technology.",
keywords = "Gene Editing/methods, Humans, DNA Glycosylases/metabolism, CRISPR-Cas Systems, Animals, DNA/genetics, редактирование генома, базовые редакторы, ДНК-деаминазы, ДНК-гликозилазы, белковая инженерия",
author = "Kulishova, {Liliya M.} and Zharkov, {Dmitry O.}",
note = "Kulishova, L.M., Zharkov, D.O. Glycosylase Base Editors: New Tools for Genome Editing. Biochemistry Moscow 91, 1093–1113 (2026). https://doi.org/10.1134/S0006297926601073 The work was financially supported by the grant from the Russian Science Foundation no. 21-64-00017p. Structural analysis was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. 125012300657-2).",
year = "2026",
month = jul,
day = "31",
doi = "10.1134/S0006297926601073",
language = "English",
volume = "91",
pages = "1093--1113",
journal = "Biochemistry (Moscow)",
issn = "0006-2979",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "7",

}

RIS

TY - JOUR

T1 - Glycosylase Base Editors

T2 - New Tools for Genome Editing

AU - Kulishova, Liliya M.

AU - Zharkov, Dmitry O.

N1 - Kulishova, L.M., Zharkov, D.O. Glycosylase Base Editors: New Tools for Genome Editing. Biochemistry Moscow 91, 1093–1113 (2026). https://doi.org/10.1134/S0006297926601073 The work was financially supported by the grant from the Russian Science Foundation no. 21-64-00017p. Structural analysis was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. 125012300657-2).

PY - 2026/7/31

Y1 - 2026/7/31

N2 - Genome editing using the CRISPR/Cas9 system has become a staple of modern genome manipulation. In its original form, editing involved introducing double-strand breaks into DNA, which can cause genomic instability. The appearance of the first base editors in 2016 expanded the range of editing technologies and enabled single-nucleotide changes to be introduced into the genome through deamination of nucleobases, bypassing the double-strand break stage. Further development of base editors involves the incorporation of additional modules, DNA glycosylases, that can remove modified or even normal nucleobases and create non-instructive apurinic/apyrimidinic sites in DNA, significantly expanding the range of available single-nucleotide substitutions. This review examines the operating principles of the glycosylase base editors, the main limitations of these genome manipulation tools, and promising areas for the development of this technology.

AB - Genome editing using the CRISPR/Cas9 system has become a staple of modern genome manipulation. In its original form, editing involved introducing double-strand breaks into DNA, which can cause genomic instability. The appearance of the first base editors in 2016 expanded the range of editing technologies and enabled single-nucleotide changes to be introduced into the genome through deamination of nucleobases, bypassing the double-strand break stage. Further development of base editors involves the incorporation of additional modules, DNA glycosylases, that can remove modified or even normal nucleobases and create non-instructive apurinic/apyrimidinic sites in DNA, significantly expanding the range of available single-nucleotide substitutions. This review examines the operating principles of the glycosylase base editors, the main limitations of these genome manipulation tools, and promising areas for the development of this technology.

KW - Gene Editing/methods

KW - Humans

KW - DNA Glycosylases/metabolism

KW - CRISPR-Cas Systems

KW - Animals

KW - DNA/genetics

KW - редактирование генома

KW - базовые редакторы

KW - ДНК-деаминазы

KW - ДНК-гликозилазы

KW - белковая инженерия

UR - https://www.scopus.com/pages/publications/105046274904

U2 - 10.1134/S0006297926601073

DO - 10.1134/S0006297926601073

M3 - Review article

C2 - 42633712

VL - 91

SP - 1093

EP - 1113

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - 7

ER -

ID: 83183962