Genomic Characterization of a Novel Yezo Virus Revealed in Ixodes pavlovskyi Tick Virome in Western Siberia. / Apanasevich, Maxim; Dubovitskiy, Nikita; Derko, Anastasiya et al.
In: Viruses, Vol. 17, No. 10, 11.10.2025, p. 1362.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Genomic Characterization of a Novel Yezo Virus Revealed in Ixodes pavlovskyi Tick Virome in Western Siberia
AU - Apanasevich, Maxim
AU - Dubovitskiy, Nikita
AU - Derko, Anastasiya
AU - Khozyainova, Anna
AU - Tarasov, Alexander
AU - Kokhanenko, Alina
AU - Artemov, Gleb
AU - Denisov, Evgeny
AU - Shestopalov, Alexander
AU - Sharshov, Kirill
N1 - Construction of the phylogenetic trees was carried out using sequences deposited in GenBank. We are grateful to the GenBank database (https://www.ncbi.nlm.nih.gov/ accessed on 8 July 2025) and to the authors who provided sequence information. We are grateful to our colleagues from Core Facility “Medical genomics” of Tomsk National Research Medical Center of the Russian Academy of Sciences (Tomsk, Russia) for sequencing on a Genolab M platform. We are grateful to the reviewers for their significant assistance in working on the manuscript. Genomic Characterization of a Novel Yezo Virus Revealed in Ixodes pavlovskyi Tick Virome in Western Siberia / M. Apanasevich, N. Dubovitskiy, A. Derko [et al.] // Viruses. – 2025. – Vol. 17, No. 10. – DOI 10.3390/v17101362.
PY - 2025/10/11
Y1 - 2025/10/11
N2 - Ixodid ticks are blood-sucking ectoparasites of vertebrates. They constitute an integral part of natural foci and are responsible for the worldwide transmission of infections to humans, which can result in severe symptoms. For instance, the Tomsk region, where three abundant tick species (Dermacentor reticulatus, Ixodes pavlovskyi, I. persulcatus) occur, is an endemic area for tick-borne encephalitis virus (TBEV). An increasing number of novel infectious agents carried by ticks have been identified using metagenomic sequencing. A notable example is the Yezo virus (Orthonairovirus yezoense, YEZV), which was discovered in patients with fever after tick bites in Japan and China between 2014 and 2025. For the first time, we have performed metagenomic sequencing of the virome of ticks collected in the Tomsk region. In a sample obtained from a pool of I. pavlovskyi ticks, all three segments of the YEZV genome were detected. The phylogenetic analysis showed that the newly identified isolate formed a sister group to previously described virus isolates, indicating the presence of a new genetic variant. This study presents the first report of YEZV detection in I. pavlovskyi ticks in the Tomsk region, thereby expanding the geographical range and number of vector species for YEZV and highlighting the importance of monitoring viral agents circulating among ticks in Western Siberia.
AB - Ixodid ticks are blood-sucking ectoparasites of vertebrates. They constitute an integral part of natural foci and are responsible for the worldwide transmission of infections to humans, which can result in severe symptoms. For instance, the Tomsk region, where three abundant tick species (Dermacentor reticulatus, Ixodes pavlovskyi, I. persulcatus) occur, is an endemic area for tick-borne encephalitis virus (TBEV). An increasing number of novel infectious agents carried by ticks have been identified using metagenomic sequencing. A notable example is the Yezo virus (Orthonairovirus yezoense, YEZV), which was discovered in patients with fever after tick bites in Japan and China between 2014 and 2025. For the first time, we have performed metagenomic sequencing of the virome of ticks collected in the Tomsk region. In a sample obtained from a pool of I. pavlovskyi ticks, all three segments of the YEZV genome were detected. The phylogenetic analysis showed that the newly identified isolate formed a sister group to previously described virus isolates, indicating the presence of a new genetic variant. This study presents the first report of YEZV detection in I. pavlovskyi ticks in the Tomsk region, thereby expanding the geographical range and number of vector species for YEZV and highlighting the importance of monitoring viral agents circulating among ticks in Western Siberia.
KW - virome
KW - metagenomic sequencing
KW - tick-borne viruses
KW - Ixodes pavlovskyi
KW - Orthonaitovirus yezoense
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105020191813&origin=inward
UR - https://elibrary.ru/item.asp?id=83014879
U2 - 10.3390/v17101362
DO - 10.3390/v17101362
M3 - Article
VL - 17
SP - 1362
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 10
ER -
ID: 71755653