Standard

Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains. / Soloveva, Irina V.; Ilyicheva, Tatyana N.; Marchenko, Vasiliy Yu et al.

In: BioMed Research International, Vol. 2021, 6662027, 2021.

Research output: Contribution to journalArticlepeer-review

Harvard

Soloveva, IV, Ilyicheva, TN, Marchenko, VY, Pyankov, OV, Tochilina, AG, Belova, IV, Zhirnov, VA, Bormotov, NI, Skarnovich, MO, Durymanov, AG, Molodtsova, SB, Filippova, EI, Ovchinnikova, AS, Magerramova, AV, Ryzhikov, AB & Maksyutov, RA 2021, 'Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains', BioMed Research International, vol. 2021, 6662027. https://doi.org/10.1155/2021/6662027

APA

Soloveva, I. V., Ilyicheva, T. N., Marchenko, V. Y., Pyankov, O. V., Tochilina, A. G., Belova, I. V., Zhirnov, V. A., Bormotov, N. I., Skarnovich, M. O., Durymanov, A. G., Molodtsova, S. B., Filippova, E. I., Ovchinnikova, A. S., Magerramova, A. V., Ryzhikov, A. B., & Maksyutov, R. A. (2021). Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains. BioMed Research International, 2021, [6662027]. https://doi.org/10.1155/2021/6662027

Vancouver

Soloveva IV, Ilyicheva TN, Marchenko VY, Pyankov OV, Tochilina AG, Belova IV et al. Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains. BioMed Research International. 2021;2021:6662027. doi: 10.1155/2021/6662027

Author

Soloveva, Irina V. ; Ilyicheva, Tatyana N. ; Marchenko, Vasiliy Yu et al. / Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains. In: BioMed Research International. 2021 ; Vol. 2021.

BibTeX

@article{846f0c3bf58a4300a8ad0f24c4cf949e,
title = "Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains",
abstract = "Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine. ",
keywords = "Animals, Bifidobacterium longum/genetics, COVID-19/metabolism, Chlorocebus aethiops, Dogs, High-Throughput Nucleotide Sequencing, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human, Lactobacillus/genetics, Madin Darby Canine Kidney Cells, Probiotics/pharmacology, SARS-CoV-2/metabolism, Vero Cells",
author = "Soloveva, {Irina V.} and Ilyicheva, {Tatyana N.} and Marchenko, {Vasiliy Yu} and Pyankov, {Oleg V.} and Tochilina, {Anna G.} and Belova, {Irina V.} and Zhirnov, {Vladimir A.} and Bormotov, {Nikolay I.} and Skarnovich, {Maksim O.} and Durymanov, {Aleksander G.} and Molodtsova, {Svetlana B.} and Filippova, {Ekaterina I.} and Ovchinnikova, {Alena S.} and Magerramova, {Anastasia V.} and Ryzhikov, {Alexander B.} and Maksyutov, {Rinat A.}",
note = "Publisher Copyright: {\textcopyright} 2021 Irina V. Soloveva et al.",
year = "2021",
doi = "10.1155/2021/6662027",
language = "English",
volume = "2021",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains

AU - Soloveva, Irina V.

AU - Ilyicheva, Tatyana N.

AU - Marchenko, Vasiliy Yu

AU - Pyankov, Oleg V.

AU - Tochilina, Anna G.

AU - Belova, Irina V.

AU - Zhirnov, Vladimir A.

AU - Bormotov, Nikolay I.

AU - Skarnovich, Maksim O.

AU - Durymanov, Aleksander G.

AU - Molodtsova, Svetlana B.

AU - Filippova, Ekaterina I.

AU - Ovchinnikova, Alena S.

AU - Magerramova, Anastasia V.

AU - Ryzhikov, Alexander B.

AU - Maksyutov, Rinat A.

N1 - Publisher Copyright: © 2021 Irina V. Soloveva et al.

PY - 2021

Y1 - 2021

N2 - Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.

AB - Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.

KW - Animals

KW - Bifidobacterium longum/genetics

KW - COVID-19/metabolism

KW - Chlorocebus aethiops

KW - Dogs

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Influenza A Virus, H1N1 Subtype

KW - Influenza, Human

KW - Lactobacillus/genetics

KW - Madin Darby Canine Kidney Cells

KW - Probiotics/pharmacology

KW - SARS-CoV-2/metabolism

KW - Vero Cells

UR - http://www.scopus.com/inward/record.url?scp=85109313780&partnerID=8YFLogxK

UR - https://www.elibrary.ru/item.asp?id=46372060

U2 - 10.1155/2021/6662027

DO - 10.1155/2021/6662027

M3 - Article

C2 - 34258278

AN - SCOPUS:85109313780

VL - 2021

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

M1 - 6662027

ER -

ID: 34153522