Research output: Contribution to journal › Article › peer-review
Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains. / Soloveva, Irina V.; Ilyicheva, Tatyana N.; Marchenko, Vasiliy Yu et al.
In: BioMed Research International, Vol. 2021, 6662027, 2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Genome Features and in Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains
AU - Soloveva, Irina V.
AU - Ilyicheva, Tatyana N.
AU - Marchenko, Vasiliy Yu
AU - Pyankov, Oleg V.
AU - Tochilina, Anna G.
AU - Belova, Irina V.
AU - Zhirnov, Vladimir A.
AU - Bormotov, Nikolay I.
AU - Skarnovich, Maksim O.
AU - Durymanov, Aleksander G.
AU - Molodtsova, Svetlana B.
AU - Filippova, Ekaterina I.
AU - Ovchinnikova, Alena S.
AU - Magerramova, Anastasia V.
AU - Ryzhikov, Alexander B.
AU - Maksyutov, Rinat A.
N1 - Publisher Copyright: © 2021 Irina V. Soloveva et al.
PY - 2021
Y1 - 2021
N2 - Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.
AB - Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.
KW - Animals
KW - Bifidobacterium longum/genetics
KW - COVID-19/metabolism
KW - Chlorocebus aethiops
KW - Dogs
KW - High-Throughput Nucleotide Sequencing
KW - Humans
KW - Influenza A Virus, H1N1 Subtype
KW - Influenza, Human
KW - Lactobacillus/genetics
KW - Madin Darby Canine Kidney Cells
KW - Probiotics/pharmacology
KW - SARS-CoV-2/metabolism
KW - Vero Cells
UR - http://www.scopus.com/inward/record.url?scp=85109313780&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=46372060
U2 - 10.1155/2021/6662027
DO - 10.1155/2021/6662027
M3 - Article
C2 - 34258278
AN - SCOPUS:85109313780
VL - 2021
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
M1 - 6662027
ER -
ID: 34153522