Research output: Contribution to journal › Article › peer-review
General Study and Gene Expression Profiling of Endotheliocytes Cultivated on Electrospun Materials. / Stepanova, Alena O.; Laktionov, Petr P.; Cherepanova, Anna V. et al.
In: Materials, Vol. 12, No. 24, 4082, 06.12.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - General Study and Gene Expression Profiling of Endotheliocytes Cultivated on Electrospun Materials
AU - Stepanova, Alena O.
AU - Laktionov, Petr P.
AU - Cherepanova, Anna V.
AU - Chernonosova, Vera S.
AU - Shevelev, Georgiy Yu
AU - Zaporozhchenko, Ivan A.
AU - Karaskov, Alexander M.
AU - Laktionov, Pavel P.
N1 - Publisher Copyright: © 2019 by the authors. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019/12/6
Y1 - 2019/12/6
N2 - Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. Here, we have cultivated human endothelial cells (HUVEC) on different 3D matrices to assess cell proliferation, gene expression and select the best substrate for endothelization. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL), and blends of PCL with gelatin (Gl) in hexafluoroisopropanol. Structure and surface properties of 3D matrices were characterized by SEM, AFM, and sessile drop analysis. Cell adhesion, viability, and proliferation were studied by SEM, Alamar Blue staining, and 5-ethynyl-2'-deoxyuridine (EdU) assay. Gene expression profiling was done on an Illumina HiSeq 2500 platform. Obtained data indicated that 3D matrices produced from PCL with Gl and treated with glutaraldehyde provide the most suitable support for HUVEC adhesion and proliferation. Transcriptome sequencing has demonstrated a minimal difference of gene expression profile in HUVEC cultivated on the surface of these matrices as compared to tissue culture plastic, thus confirming these matrices as the best support for endothelization.
AB - Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. Here, we have cultivated human endothelial cells (HUVEC) on different 3D matrices to assess cell proliferation, gene expression and select the best substrate for endothelization. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL), and blends of PCL with gelatin (Gl) in hexafluoroisopropanol. Structure and surface properties of 3D matrices were characterized by SEM, AFM, and sessile drop analysis. Cell adhesion, viability, and proliferation were studied by SEM, Alamar Blue staining, and 5-ethynyl-2'-deoxyuridine (EdU) assay. Gene expression profiling was done on an Illumina HiSeq 2500 platform. Obtained data indicated that 3D matrices produced from PCL with Gl and treated with glutaraldehyde provide the most suitable support for HUVEC adhesion and proliferation. Transcriptome sequencing has demonstrated a minimal difference of gene expression profile in HUVEC cultivated on the surface of these matrices as compared to tissue culture plastic, thus confirming these matrices as the best support for endothelization.
KW - 3D matrices
KW - Electrospinning
KW - Endothelization
KW - Polycaprolactone
KW - RNA sequencing
KW - Vascular grafts
UR - http://www.scopus.com/inward/record.url?scp=85079683379&partnerID=8YFLogxK
U2 - 10.3390/ma12244082
DO - 10.3390/ma12244082
M3 - Article
C2 - 31817735
VL - 12
JO - Materials
JF - Materials
SN - 1996-1944
IS - 24
M1 - 4082
ER -
ID: 23287839