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Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells. / Potter, Ekaterina A.; Dolgova, Evgenia V.; Proskurina, Anastasia S. et al.

In: Oncotarget, Vol. 8, No. 6, 07.02.2017, p. 9425-9441.

Research output: Contribution to journal › Article › peer-review

Harvard

Potter, EA, Dolgova, EV, Proskurina, AS, Efremov, YR, Minkevich, AM, Rozanov, AS, Peltek, SE, Nikolin, VP, Popova, NA, Seledtsov, IA, Molodtsov, VV, Zavyalov, EL, Taranov, OS, Baiborodin, SI, Ostanin, AA, Chernykh, ER, Kolchanov, NA & Bogachev, SS 2017, 'Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells', Oncotarget, vol. 8, no. 6, pp. 9425-9441. https://doi.org/10.18632/oncotarget.14116

APA

Potter, E. A., Dolgova, E. V., Proskurina, A. S., Efremov, Y. R., Minkevich, A. M., Rozanov, A. S., Peltek, S. E., Nikolin, V. P., Popova, N. A., Seledtsov, I. A., Molodtsov, V. V., Zavyalov, E. L., Taranov, O. S., Baiborodin, S. I., Ostanin, A. A., Chernykh, E. R., Kolchanov, N. A., & Bogachev, S. S. (2017). Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells. Oncotarget, 8(6), 9425-9441. https://doi.org/10.18632/oncotarget.14116

Vancouver

Potter EA, Dolgova EV, Proskurina AS, Efremov YR, Minkevich AM, Rozanov AS et al. Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells. Oncotarget. 2017 Feb 7;8(6):9425-9441. doi: 10.18632/oncotarget.14116

Author

Potter, Ekaterina A. ; Dolgova, Evgenia V. ; Proskurina, Anastasia S. et al. / Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells. In: Oncotarget. 2017 ; Vol. 8, No. 6. pp. 9425-9441.

BibTeX

@article{557d4d7f051d4e6cad26efbb88c5fa4f,
title = "Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells",
abstract = "Using the ability of poorly differentiated cells to natively internalize fragments of extracellular double-stranded DNA as a marker, we isolated a tumorigenic subpopulation present in Krebs-2 ascites that demonstrated the features of tumor-inducing cancer stem cells. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, we identified a set of 168 genes specifically expressed in TAMRA-positive cells (tumor-initiating stem cells), these genes remaining silent in TAMRA-negative cancer cells. TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells. The observed expression differences between TAMRA+ and TAMRA- cells were validated by Real Time PCR. The results obtained corroborated the biological data that TAMRA+ murine Krebs-2 tumor cells are tumor-initiating stem cells. The approach developed can be applied to profile any poorly differentiated cell types that are capable of immanent internalization of double-stranded DNA.",
keywords = "DNA internalization, Real Time PCR, RNAseq, TAMRA, Tumor-initiating stem cells, Gene Expression Profiling/methods, Rhodamines/metabolism, Signal Transduction, Gene Expression Regulation, Neoplastic, Neoplastic Stem Cells/metabolism, Transcriptome, Carcinoma, Krebs 2/genetics, Gene Regulatory Networks, Biomarkers, Tumor/genetics, DNA/genetics, Phenotype, Animals, Sequence Analysis, RNA, Cell Differentiation, High-Throughput Nucleotide Sequencing, Fluorescent Dyes/metabolism, Alu Elements, Real-Time Polymerase Chain Reaction, SIGNALING PATHWAYS, COLONY-STIMULATING FACTOR, DRUG-RESISTANCE, BREAST-CANCER, IN-VITRO, tumor-initiating stem cells, PROSTATE-CANCER, ANTIAPOPTOTIC GENES, CANCER-ASSOCIATED FIBROBLASTS, SELF-RENEWAL, ACUTE MYELOID-LEUKEMIA",
author = "Potter, {Ekaterina A.} and Dolgova, {Evgenia V.} and Proskurina, {Anastasia S.} and Efremov, {Yaroslav R.} and Minkevich, {Alexandra M.} and Rozanov, {Aleksey S.} and Peltek, {Sergey E.} and Nikolin, {Valeriy P.} and Popova, {Nelly A.} and Seledtsov, {Igor A.} and Molodtsov, {Vladimir V.} and Zavyalov, {Evgeniy L.} and Taranov, {Oleg S.} and Baiborodin, {Sergey I.} and Ostanin, {Alexander A.} and Chernykh, {Elena R.} and Kolchanov, {Nikolay A.} and Bogachev, {Sergey S.}",
year = "2017",
month = feb,
day = "7",
doi = "10.18632/oncotarget.14116",
language = "English",
volume = "8",
pages = "9425--9441",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "6",

}

RIS

TY - JOUR

T1 - Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells

AU - Potter, Ekaterina A.

AU - Dolgova, Evgenia V.

AU - Proskurina, Anastasia S.

AU - Efremov, Yaroslav R.

AU - Minkevich, Alexandra M.

AU - Rozanov, Aleksey S.

AU - Peltek, Sergey E.

AU - Nikolin, Valeriy P.

AU - Popova, Nelly A.

AU - Seledtsov, Igor A.

AU - Molodtsov, Vladimir V.

AU - Zavyalov, Evgeniy L.

AU - Taranov, Oleg S.

AU - Baiborodin, Sergey I.

AU - Ostanin, Alexander A.

AU - Chernykh, Elena R.

AU - Kolchanov, Nikolay A.

AU - Bogachev, Sergey S.

PY - 2017/2/7

Y1 - 2017/2/7

N2 - Using the ability of poorly differentiated cells to natively internalize fragments of extracellular double-stranded DNA as a marker, we isolated a tumorigenic subpopulation present in Krebs-2 ascites that demonstrated the features of tumor-inducing cancer stem cells. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, we identified a set of 168 genes specifically expressed in TAMRA-positive cells (tumor-initiating stem cells), these genes remaining silent in TAMRA-negative cancer cells. TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells. The observed expression differences between TAMRA+ and TAMRA- cells were validated by Real Time PCR. The results obtained corroborated the biological data that TAMRA+ murine Krebs-2 tumor cells are tumor-initiating stem cells. The approach developed can be applied to profile any poorly differentiated cell types that are capable of immanent internalization of double-stranded DNA.

AB - Using the ability of poorly differentiated cells to natively internalize fragments of extracellular double-stranded DNA as a marker, we isolated a tumorigenic subpopulation present in Krebs-2 ascites that demonstrated the features of tumor-inducing cancer stem cells. Having combined TAMRA-labeled DNA probe and the power of RNA-seq technology, we identified a set of 168 genes specifically expressed in TAMRA-positive cells (tumor-initiating stem cells), these genes remaining silent in TAMRA-negative cancer cells. TAMRA+ cells displayed gene expression signatures characteristic of both stem cells and cancer cells. The observed expression differences between TAMRA+ and TAMRA- cells were validated by Real Time PCR. The results obtained corroborated the biological data that TAMRA+ murine Krebs-2 tumor cells are tumor-initiating stem cells. The approach developed can be applied to profile any poorly differentiated cell types that are capable of immanent internalization of double-stranded DNA.

KW - DNA internalization

KW - Real Time PCR

KW - RNAseq

KW - TAMRA

KW - Tumor-initiating stem cells

KW - Gene Expression Profiling/methods

KW - Rhodamines/metabolism

KW - Signal Transduction

KW - Gene Expression Regulation, Neoplastic

KW - Neoplastic Stem Cells/metabolism

KW - Transcriptome

KW - Carcinoma, Krebs 2/genetics

KW - Gene Regulatory Networks

KW - Biomarkers, Tumor/genetics

KW - DNA/genetics

KW - Phenotype

KW - Animals

KW - Sequence Analysis, RNA

KW - Cell Differentiation

KW - High-Throughput Nucleotide Sequencing

KW - Fluorescent Dyes/metabolism

KW - Alu Elements

KW - Real-Time Polymerase Chain Reaction

KW - SIGNALING PATHWAYS

KW - COLONY-STIMULATING FACTOR

KW - DRUG-RESISTANCE

KW - BREAST-CANCER

KW - IN-VITRO

KW - tumor-initiating stem cells

KW - PROSTATE-CANCER

KW - ANTIAPOPTOTIC GENES

KW - CANCER-ASSOCIATED FIBROBLASTS

KW - SELF-RENEWAL

KW - ACUTE MYELOID-LEUKEMIA

UR - http://www.scopus.com/inward/record.url?scp=85012025889&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.14116

DO - 10.18632/oncotarget.14116

M3 - Article

C2 - 28031533

AN - SCOPUS:85012025889

VL - 8

SP - 9425

EP - 9441

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 6

ER -

ID: 10311690