Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis. / Shalaev, Pavel V.; Bondina, Ekaterina V.; Dolgushin, Sergey A. et al.
Clinical and Preclinical Optical Diagnostics II. ed. / J. Quincy Brown; Ton G. van Leeuwen. SPIE, 2019. 110731W (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11073).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
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TY - GEN
T1 - Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis
AU - Shalaev, Pavel V.
AU - Bondina, Ekaterina V.
AU - Dolgushin, Sergey A.
AU - Semeykina, Victoria S.
AU - Sankova, Natalya N.
AU - Parkhomchuk, Ekaterina V.
N1 - Publisher Copyright: © 2019 SPIE. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.
AB - Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.
KW - Cyanine dyes
KW - Immunofluorescence analysis
KW - Polystyrene microspheres
KW - polystyrene microspheres
KW - immunofluorescence analysis
KW - cyanine dyes
UR - http://www.scopus.com/inward/record.url?scp=85073749856&partnerID=8YFLogxK
U2 - 10.1117/12.2526387
DO - 10.1117/12.2526387
M3 - Conference contribution
AN - SCOPUS:85073749856
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Clinical and Preclinical Optical Diagnostics II
A2 - Brown, J. Quincy
A2 - van Leeuwen, Ton G.
PB - SPIE
T2 - Clinical and Preclinical Optical Diagnostics II 2019
Y2 - 23 June 2019 through 25 June 2019
ER -
ID: 21937851