Research output: Contribution to journal › Article › peer-review
Fluorescent bacterial biosensor E. Coli/pTdcR-TurboYFP sensitive to terahertz radiation. / Serdyukov, Danil S.; Goryachkovskaya, Tatiana N.; Mescheryakova, Irina A. et al.
In: Biomedical Optics Express, Vol. 12, No. 2, 2021, p. 705-721.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Fluorescent bacterial biosensor E. Coli/pTdcR-TurboYFP sensitive to terahertz radiation
AU - Serdyukov, Danil S.
AU - Goryachkovskaya, Tatiana N.
AU - Mescheryakova, Irina A.
AU - Kuznetsov, Sergei A.
AU - Popik, Vasiliy M.
AU - Peltek, Sergey E.
N1 - Publisher Copyright: © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
PY - 2021
Y1 - 2021
N2 - A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli (E. coli) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli.
AB - A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli (E. coli) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli.
UR - http://www.scopus.com/inward/record.url?scp=85100104825&partnerID=8YFLogxK
U2 - 10.1364/BOE.412074
DO - 10.1364/BOE.412074
M3 - Article
C2 - 33680537
AN - SCOPUS:85100104825
VL - 12
SP - 705
EP - 721
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 2
ER -
ID: 27710644