Research output: Contribution to journal › Article › peer-review
First cytogenetic analysis of lesser gymnures (Mammalia, Galericidae, Hylomys) from Vietnam. / Pavlova, Svetlana V.; Biltueva, Larisa S.; Romanenko, Svetlana A. et al.
In: Comparative Cytogenetics, Vol. 12, No. 3, 23.08.2018, p. 361-372.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - First cytogenetic analysis of lesser gymnures (Mammalia, Galericidae, Hylomys) from Vietnam
AU - Pavlova, Svetlana V.
AU - Biltueva, Larisa S.
AU - Romanenko, Svetlana A.
AU - Lemskaya, Natalya A.
AU - Shchinov, Anton V.
AU - Abramov, Alexei V.
AU - Rozhnov, Viatcheslav V.
N1 - Publisher Copyright: © Svetlana V. Pavlova et al.
PY - 2018/8/23
Y1 - 2018/8/23
N2 - Gymnures are an ancient group of small insectivorous mammals and are characterized by a controversial taxonomic status and the lack of a description of karyotypes for certain species. In this study, conventional cytogenetic techniques (Giemsa, CBG- and GTG-banding, Ag-NOR), CMA3-DAPI staining, and fluorescent in situ hybridization (FISH) with telomeric DNA probes were used to examine for the first time the karyotypes of lesser gymnures of group Hylomys suillus Müller, 1840 from northern and southern Vietnam. All studied specimens had karyotypes with 2n=48, NFa=64. C-positive heterochromatic blocks existed in centromeric regions of 7 bi-armed autosomes and the submetacentric X chromosome. The Y chromosome is a C-positive and dot-like. The nucleolus organizer regions resided terminally on the short arms of 2 small bi-armed pairs. Positive signals at the telomeres of all chromosomes were revealed by FISH. CMA3-positive blocks were localized on the telomeric and pericentric regions of most bi-armed and acrocentric chromosomes. Despite the large genetic distances between Hylomys Müller, 1840, lesser gymnures from H. suillus-group from northern and southern Vietnam have similar karyotypic characteristics.
AB - Gymnures are an ancient group of small insectivorous mammals and are characterized by a controversial taxonomic status and the lack of a description of karyotypes for certain species. In this study, conventional cytogenetic techniques (Giemsa, CBG- and GTG-banding, Ag-NOR), CMA3-DAPI staining, and fluorescent in situ hybridization (FISH) with telomeric DNA probes were used to examine for the first time the karyotypes of lesser gymnures of group Hylomys suillus Müller, 1840 from northern and southern Vietnam. All studied specimens had karyotypes with 2n=48, NFa=64. C-positive heterochromatic blocks existed in centromeric regions of 7 bi-armed autosomes and the submetacentric X chromosome. The Y chromosome is a C-positive and dot-like. The nucleolus organizer regions resided terminally on the short arms of 2 small bi-armed pairs. Positive signals at the telomeres of all chromosomes were revealed by FISH. CMA3-positive blocks were localized on the telomeric and pericentric regions of most bi-armed and acrocentric chromosomes. Despite the large genetic distances between Hylomys Müller, 1840, lesser gymnures from H. suillus-group from northern and southern Vietnam have similar karyotypic characteristics.
KW - Cell culture
KW - Cryoconservation
KW - FISH
KW - Insectivorous mammals
KW - Karyotype
KW - Telomeric sequence
KW - insectivorous mammals
KW - ERINACEOMORPHA
KW - cryoconservation
KW - cell culture
KW - karyotypc
KW - INSECTIVORA
KW - telomeric sequence
UR - http://www.scopus.com/inward/record.url?scp=85058000959&partnerID=8YFLogxK
U2 - 10.3897/CompCytogen.v12i3.27207
DO - 10.3897/CompCytogen.v12i3.27207
M3 - Article
C2 - 30275928
AN - SCOPUS:85058000959
VL - 12
SP - 361
EP - 372
JO - Comparative Cytogenetics
JF - Comparative Cytogenetics
SN - 1993-0771
IS - 3
ER -
ID: 18186497