Research output: Contribution to journal › Article › peer-review
Expression analysis of microRNA and lncRNA in visceral adipose tissue of obese and non-obese individuals. / Bairqdar, A.; Ivanoshchuk, D. E.; Tuzovskaya, O. V. et al.
In: Vavilovskii Zhurnal Genetiki i Selektsii, Vol. 29, No. 3, 2025, p. 448-457.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Expression analysis of microRNA and lncRNA in visceral adipose tissue of obese and non-obese individuals
AU - Bairqdar, A.
AU - Ivanoshchuk, D. E.
AU - Tuzovskaya, O. V.
AU - Shirokova, N. S.
AU - Kashtanova, E. V.
AU - Polonskaya, Y. V.
AU - Ragino, Y. I.
AU - Shakhtshneider, E. V.
N1 - The molecular genetic testing was carried out within the framework of the main topic of state assignment No. FWNR-2025-0006. Expression analysis of microRNA and lncRNA in visceral adipose tissue of obese and non-obese individuals / Bairqdar A., Ivanoshchuk D.E., Tuzovskaya O.V., Shirokova N.S., Kashtanova E.V., Polonskaya Y.V., Ragino Y.I., Shakhtshneider E.V. // Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov J Genet Breed. 2025. - №29 (3). С. 448-457.
PY - 2025
Y1 - 2025
N2 - Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play important roles in all biological processes, including adipogenesis, lipid metabolism, and insulin response. Analyzing expression patterns of lncRNAs and miRNAs in human visceral fat tissue can enhance our understanding of their roles in metabolic disorders. Our research aims to investigate the expression of lncRNAs (ASMER1, SNHG9, P5549, P19461, and GAS5) and miRNAs (miR-26A, miR-222, miR-221, and miR-155) in visceral adipose tissues of individuals with abdominal obesity (n = 70) compared to their levels in non-obese participants (n = 31), using Real-Time PCR. Among the tested miRNAs, only miR-26A was significantly downregulated in the visceral adipose tissue of obese individuals, with no significant change in the expression of miR-26A in obese people with or without type 2 diabetes. Similarly, of the tested lncRNAs, only GAS5 showed significantly higher expression levels in obese patients with type 2 diabetes (T2D) (n = 10) compared to obese patients without T2D (n = 60). To test possible interactions between the analyzed non-coding RNAs, we used Spearman’s bivariate correlation test. GAS5 expression levels showed a weak negative correlation (p < 0.05, rs = 0.25) with miR-155 levels in obese patients only. Conversely, a strong positive correlation (p < 0.01, rs = 0.92) between SNHG9 and GAS5 was found in the non-obese group, with a weaker correlation in abdominally obese patients (p < 0.01, rs = 0.67); additionally, miR-26A and miR-155 levels were moderately correlated in the non-obese group (p < 0.05, rs = 0.47) and were found to correlate weakly in obese patients (p < 0.05, rs = 0.26). Our results showed that abdominally obese participants demonstrated higher expression levels of miR-26A in visceral adipose tissue and a significantly lower correlation between GAS5 and SNHG9 expression when compared to non-obese subjects.
AB - Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play important roles in all biological processes, including adipogenesis, lipid metabolism, and insulin response. Analyzing expression patterns of lncRNAs and miRNAs in human visceral fat tissue can enhance our understanding of their roles in metabolic disorders. Our research aims to investigate the expression of lncRNAs (ASMER1, SNHG9, P5549, P19461, and GAS5) and miRNAs (miR-26A, miR-222, miR-221, and miR-155) in visceral adipose tissues of individuals with abdominal obesity (n = 70) compared to their levels in non-obese participants (n = 31), using Real-Time PCR. Among the tested miRNAs, only miR-26A was significantly downregulated in the visceral adipose tissue of obese individuals, with no significant change in the expression of miR-26A in obese people with or without type 2 diabetes. Similarly, of the tested lncRNAs, only GAS5 showed significantly higher expression levels in obese patients with type 2 diabetes (T2D) (n = 10) compared to obese patients without T2D (n = 60). To test possible interactions between the analyzed non-coding RNAs, we used Spearman’s bivariate correlation test. GAS5 expression levels showed a weak negative correlation (p < 0.05, rs = 0.25) with miR-155 levels in obese patients only. Conversely, a strong positive correlation (p < 0.01, rs = 0.92) between SNHG9 and GAS5 was found in the non-obese group, with a weaker correlation in abdominally obese patients (p < 0.01, rs = 0.67); additionally, miR-26A and miR-155 levels were moderately correlated in the non-obese group (p < 0.05, rs = 0.47) and were found to correlate weakly in obese patients (p < 0.05, rs = 0.26). Our results showed that abdominally obese participants demonstrated higher expression levels of miR-26A in visceral adipose tissue and a significantly lower correlation between GAS5 and SNHG9 expression when compared to non-obese subjects.
KW - GAS5
KW - SNHG9
KW - abdominal obesity
KW - long non-coding RNA
KW - miR-155
KW - miR-26A
KW - microRNA
KW - visceral adipose tissues
UR - https://www.mendeley.com/catalogue/f3b5341a-35a6-37f6-8dea-4d8747ec092f/
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105008016149&origin=inward&txGid=a84d3aeb0e88841e885ed086df1863c8
UR - https://elibrary.ru/item.asp?id=82493232
U2 - 10.18699/vjgb-25-48
DO - 10.18699/vjgb-25-48
M3 - Article
VL - 29
SP - 448
EP - 457
JO - Вавиловский журнал генетики и селекции
JF - Вавиловский журнал генетики и селекции
SN - 2500-0462
IS - 3
ER -
ID: 68029938