Research output: Contribution to journal › Article › peer-review
Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer. / Yunusova, Natalia V.; Zambalova, Elena A.; Patysheva, Marina R. et al.
In: Asian Pacific Journal of Cancer Prevention, Vol. 22, No. 3, 3, 2021, p. 861-869.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Exosomal Protease Cargo as Prognostic Biomarker in Colorectal Cancer
AU - Yunusova, Natalia V.
AU - Zambalova, Elena A.
AU - Patysheva, Marina R.
AU - Kolegova, Elena S.
AU - Afanas’ev, Sergey G.
AU - Cheremisina, Olga V.
AU - Grigor’eva, Alina E.
AU - Tamkovich, Svetlana N.
AU - Kondakova, Irina V.
N1 - Funding Information: This work supported by the Russian Foundation for Basic Research and the Government of Tomsk region, grant 18-415-703006. Publisher Copyright: © 2021. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Objective: The aim of the study was to develop a model for predicting cancer risk in colorectal polyps’ patients (CPPs), as well as to reveal additional prognosis factors for Stage III colorectal cancer based on differences in subpopulations of tetraspanins, tetraspanin-associated and tetraspanin-non-associated proteases in blood plasma exosomes of CPPs and colorectal cancer patients (CRCPs). Methods: The subpopulations of CD151- and Tspan8-positive exosomes, the subpopulations of metalloproteinase at the surface of СD9-positive exosomes and the level of 20S proteasomes in plasma exosomes in 15 CPPs (tubulovillous adenomas) and 60 CRCPs were evaluated using flow cytometry and Western blotting. Logistic regression analysis was performed to predict cancer risk of CPPs. Results: The levels of 20S proteasomes in exosomes, MMP9+, MMP9+/MMP2+/EMMPRIN+ in CD9-positive blood plasma exosomes are associated with the risk of malignant transformation of colorectal tubulovillous adenomas. In patients with Stage III CRC, the levels of 20S proteasomes (less than 2 units) and MMP9+ subpopulations (more than 61%) in plasma exosomes are unfavorable prognostic factors for overall survival. The levels of 20S proteasomes and ADAM10+/ ADAM17- subpopulations in CD9-positive blood plasma exosomes are the most significant values for predicting relapse-free survival. Conclusion: Protease cargo in CD9-positive blood plasma exosomes is prognostic biomarker for colorectal polyps and colorectal cancer.
AB - Objective: The aim of the study was to develop a model for predicting cancer risk in colorectal polyps’ patients (CPPs), as well as to reveal additional prognosis factors for Stage III colorectal cancer based on differences in subpopulations of tetraspanins, tetraspanin-associated and tetraspanin-non-associated proteases in blood plasma exosomes of CPPs and colorectal cancer patients (CRCPs). Methods: The subpopulations of CD151- and Tspan8-positive exosomes, the subpopulations of metalloproteinase at the surface of СD9-positive exosomes and the level of 20S proteasomes in plasma exosomes in 15 CPPs (tubulovillous adenomas) and 60 CRCPs were evaluated using flow cytometry and Western blotting. Logistic regression analysis was performed to predict cancer risk of CPPs. Results: The levels of 20S proteasomes in exosomes, MMP9+, MMP9+/MMP2+/EMMPRIN+ in CD9-positive blood plasma exosomes are associated with the risk of malignant transformation of colorectal tubulovillous adenomas. In patients with Stage III CRC, the levels of 20S proteasomes (less than 2 units) and MMP9+ subpopulations (more than 61%) in plasma exosomes are unfavorable prognostic factors for overall survival. The levels of 20S proteasomes and ADAM10+/ ADAM17- subpopulations in CD9-positive blood plasma exosomes are the most significant values for predicting relapse-free survival. Conclusion: Protease cargo in CD9-positive blood plasma exosomes is prognostic biomarker for colorectal polyps and colorectal cancer.
KW - 20S proteasome
KW - CD151
KW - colorectal cancer
KW - metalloproteinases
KW - Plasma exosomes
KW - Prognosis
KW - Proteasome Endopeptidase Complex/metabolism
KW - Colonic Polyps/enzymology
KW - Humans
KW - Middle Aged
KW - Male
KW - Peptide Hydrolases/metabolism
KW - Exosomes/enzymology
KW - Tetraspanin 24/metabolism
KW - Neoplasm Grading
KW - Colorectal Neoplasms/enzymology
KW - Female
KW - Basigin/metabolism
KW - Carcinoma/enzymology
KW - Matrix Metalloproteinase 2/metabolism
KW - Matrix Metalloproteinase 9/metabolism
KW - Tetraspanins/metabolism
KW - Intestinal Polyps/enzymology
KW - Adenoma, Villous/enzymology
KW - Adenoma/enzymology
KW - Neoplasm Staging
UR - http://www.scopus.com/inward/record.url?scp=85103483848&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=46771479
U2 - 10.31557/APJCP.2021.22.3.861
DO - 10.31557/APJCP.2021.22.3.861
M3 - Article
C2 - 33773551
AN - SCOPUS:85103483848
VL - 22
SP - 861
EP - 869
JO - Asian Pacific Journal of Cancer Prevention
JF - Asian Pacific Journal of Cancer Prevention
SN - 1513-7368
IS - 3
M1 - 3
ER -
ID: 35485182