Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review
ESEEM of spin labels to study intermolecular interactions, molecular assembly and conformation. / Dzuba, Sergei A.; Marsh, Derek.
Electron Paramagnetic Resonance. ed. / Bruce C. Gilbert; Bruce C. Gilbert; Bruce C. Gilbert; Victor Chechik; Victor Chechik; Victor Chechik; Damien M. Murphy; Damien M. Murphy; Damien M. Murphy. Royal Society of Chemistry, 2015. p. 102-121 (Electron Paramagnetic Resonance; Vol. 24).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review
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TY - CHAP
T1 - ESEEM of spin labels to study intermolecular interactions, molecular assembly and conformation
AU - Dzuba, Sergei A.
AU - Marsh, Derek
PY - 2015
Y1 - 2015
N2 - Electron Spin Echo Envelope Modulation, or ESEEM, is widely used to investigate structure of the immediate surroundings of paramagnetic species. In combination with site-specific spin labeling and selective deuterium substitution, it becomes a helpful tool to study location of proteins and small peptides within membranes, interaction of small molecules with proteins, supramolecular assemblies, water accessibility to specific regions of proteins and water penetration into membranes, protein folding and secondary structures. In this review, experimental approaches and limitations, theoretical background of the method, and recent applications are discussed.
AB - Electron Spin Echo Envelope Modulation, or ESEEM, is widely used to investigate structure of the immediate surroundings of paramagnetic species. In combination with site-specific spin labeling and selective deuterium substitution, it becomes a helpful tool to study location of proteins and small peptides within membranes, interaction of small molecules with proteins, supramolecular assemblies, water accessibility to specific regions of proteins and water penetration into membranes, protein folding and secondary structures. In this review, experimental approaches and limitations, theoretical background of the method, and recent applications are discussed.
UR - http://www.scopus.com/inward/record.url?scp=84937393112&partnerID=8YFLogxK
U2 - 10.1039/9781782620280-00102
DO - 10.1039/9781782620280-00102
M3 - Chapter
AN - SCOPUS:84937393112
T3 - Electron Paramagnetic Resonance
SP - 102
EP - 121
BT - Electron Paramagnetic Resonance
A2 - Gilbert, Bruce C.
A2 - Gilbert, Bruce C.
A2 - Gilbert, Bruce C.
A2 - Chechik, Victor
A2 - Chechik, Victor
A2 - Chechik, Victor
A2 - Murphy, Damien M.
A2 - Murphy, Damien M.
A2 - Murphy, Damien M.
PB - Royal Society of Chemistry
ER -
ID: 25832151