Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA

Standard

Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA. / Korolenya, Valeria A.; Filipenko, Maxim L.; Smetanina, Mariya A.

In: Epigenomes, Vol. 9, No. 4, 53, 17.12.2025.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{307b6fb13dcf41399922d0e03386d564,

title = "Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA",

abstract = "The endothelium, as the inner layer of the vascular wall, is in constant contact with blood components, so that leukocytes have the ability to adhere to endotheliocytes and penetrate to the subendothelial space. When studying heterogenic vascular samples containing endothelial cells or pathological processes related to inflammation within the endothelium, it may be necessary to distinguish DNA by endothelial and leukocyte origin, which is possible due to its specific epigenetic modifications. To identify CpG loci that could serve as markers for endothelial cells, we searched for their distinctive stable methylated or demethylated states by applying marginal filtering (selecting CpG loci with methylation Beta values closer to 0 and 1) to the microarray data and identified 47 CpG loci with relatively stable methylation/demethylation status that differentiate endothelial (HUVEC, HCMEC, HPAEC, HPMEC, and LSEC) DNA from leukocyte (granulocytes, monocytes, and lymphocytes) DNA. In addition, we compared CpG loci with high and low levels of DNA methylation between different types of endothelial cells and leukocytes. We believe that the obtained data will hopefully facilitate further studies on endothelial dysfunction.",

keywords = "DNA methylation, endothelial cells, leukocytes, microarray analysis",

author = "Korolenya, {Valeria A.} and Filipenko, {Maxim L.} and Smetanina, {Mariya A.}",

note = "Korolenya, V.A.; Filipenko, M.L.; Smetanina, M.A. Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA. Epigenomes 2025, 9, 53. https://doi.org/10.3390/epigenomes9040053 This research was funded by the Russian Science Foundation, grant number 25-25-20187, and the Government of the Novosibirsk Region, agreement number 30-2025-001030.",

year = "2025",

month = dec,

day = "17",

doi = "10.3390/epigenomes9040053",

language = "English",

volume = "9",

journal = "Epigenomes",

issn = "2075-4655",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "4",

}

RIS

TY - JOUR

T1 - Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA

AU - Korolenya, Valeria A.

AU - Filipenko, Maxim L.

AU - Smetanina, Mariya A.

N1 - Korolenya, V.A.; Filipenko, M.L.; Smetanina, M.A. Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA. Epigenomes 2025, 9, 53. https://doi.org/10.3390/epigenomes9040053 This research was funded by the Russian Science Foundation, grant number 25-25-20187, and the Government of the Novosibirsk Region, agreement number 30-2025-001030.

PY - 2025/12/17

Y1 - 2025/12/17

N2 - The endothelium, as the inner layer of the vascular wall, is in constant contact with blood components, so that leukocytes have the ability to adhere to endotheliocytes and penetrate to the subendothelial space. When studying heterogenic vascular samples containing endothelial cells or pathological processes related to inflammation within the endothelium, it may be necessary to distinguish DNA by endothelial and leukocyte origin, which is possible due to its specific epigenetic modifications. To identify CpG loci that could serve as markers for endothelial cells, we searched for their distinctive stable methylated or demethylated states by applying marginal filtering (selecting CpG loci with methylation Beta values closer to 0 and 1) to the microarray data and identified 47 CpG loci with relatively stable methylation/demethylation status that differentiate endothelial (HUVEC, HCMEC, HPAEC, HPMEC, and LSEC) DNA from leukocyte (granulocytes, monocytes, and lymphocytes) DNA. In addition, we compared CpG loci with high and low levels of DNA methylation between different types of endothelial cells and leukocytes. We believe that the obtained data will hopefully facilitate further studies on endothelial dysfunction.

AB - The endothelium, as the inner layer of the vascular wall, is in constant contact with blood components, so that leukocytes have the ability to adhere to endotheliocytes and penetrate to the subendothelial space. When studying heterogenic vascular samples containing endothelial cells or pathological processes related to inflammation within the endothelium, it may be necessary to distinguish DNA by endothelial and leukocyte origin, which is possible due to its specific epigenetic modifications. To identify CpG loci that could serve as markers for endothelial cells, we searched for their distinctive stable methylated or demethylated states by applying marginal filtering (selecting CpG loci with methylation Beta values closer to 0 and 1) to the microarray data and identified 47 CpG loci with relatively stable methylation/demethylation status that differentiate endothelial (HUVEC, HCMEC, HPAEC, HPMEC, and LSEC) DNA from leukocyte (granulocytes, monocytes, and lymphocytes) DNA. In addition, we compared CpG loci with high and low levels of DNA methylation between different types of endothelial cells and leukocytes. We believe that the obtained data will hopefully facilitate further studies on endothelial dysfunction.

KW - DNA methylation

KW - endothelial cells

KW - leukocytes

KW - microarray analysis

UR - https://www.scopus.com/pages/publications/105025814065

U2 - 10.3390/epigenomes9040053

DO - 10.3390/epigenomes9040053

M3 - Article

VL - 9

JO - Epigenomes

JF - Epigenomes

SN - 2075-4655

IS - 4

M1 - 53

ER -

ID: 73778135