TY - JOUR

T1 - Enhancement of the Anticancer Effect during the Simultaneous Treatment of Cells by a Cold Atmospheric Plasma Jet and Gold Nanoparticles

AU - Schweigert, I.

AU - Zakrevsky, D.

AU - Milakhina, E.

AU - Gugin, P.

AU - Biryukov, M.

AU - Polyakova, A.

AU - Kryachkova, N.

AU - Gorbunova, E.

AU - Epanchintseva, A.

AU - Pyshnaya, I.

AU - Koval, O.

N1 - This work was supported by the Russian Science Foundation, grant no. 22-49-08003 (https://rscf.ru/project/22-49-08003/). Enhancement of the Anticancer Effect during the Simultaneous Treatment of Cells by a Cold Atmospheric Plasma Jet and Gold Nanoparticles / I. Schweigert, D. Zakrevsky, E. Milakhina [et al.] // Plasma Physics Reports. – 2024. – Vol. 50, No. 11. – P. 1375-1387. – DOI 10.1134/S1063780X24601597.

PY - 2024/11/1

Y1 - 2024/11/1

N2 - Abstract: Selecting the most effective and biologically safe operation regimes of a cold atmospheric plasma jet (CAPJ) is a defining factor in developing the cancer treatments based on the CAPJ. Experimentally and numerically, by changing the pulse duration of the positive pulsed voltage, we determined the optimum CAPJ regimes with regular propagation of streamers and a maximum discharge current at a temperature T < 42°C. In these regimes, the CAPJ appreciably suppresses the viability of the cancerous cells. It was shown that adding gold nanoparticles increases the cytotoxic effect of the plasma jet and decreases the viability of the NCI‑H23 epithelioid lung adenocarcinoma, the A549 lung adenocarcinoma, the BrCCh4e-134 mammary adenocarcinoma, and the cells of the uMel1 uveal melanoma. The polyethylenglycol-modified gold nanoparticles with fluorescent labels were used to visualize the absorption of the nanoparticles by the cells. It was shown that the CAPJ stimulated the penetration of the nanoparticles into the cells when they were applied to the medium immediately before the CAPJ treatment or immediately after, which indicates a short-time increase in the permeability of the cell membrane.

AB - Abstract: Selecting the most effective and biologically safe operation regimes of a cold atmospheric plasma jet (CAPJ) is a defining factor in developing the cancer treatments based on the CAPJ. Experimentally and numerically, by changing the pulse duration of the positive pulsed voltage, we determined the optimum CAPJ regimes with regular propagation of streamers and a maximum discharge current at a temperature T < 42°C. In these regimes, the CAPJ appreciably suppresses the viability of the cancerous cells. It was shown that adding gold nanoparticles increases the cytotoxic effect of the plasma jet and decreases the viability of the NCI‑H23 epithelioid lung adenocarcinoma, the A549 lung adenocarcinoma, the BrCCh4e-134 mammary adenocarcinoma, and the cells of the uMel1 uveal melanoma. The polyethylenglycol-modified gold nanoparticles with fluorescent labels were used to visualize the absorption of the nanoparticles by the cells. It was shown that the CAPJ stimulated the penetration of the nanoparticles into the cells when they were applied to the medium immediately before the CAPJ treatment or immediately after, which indicates a short-time increase in the permeability of the cell membrane.

KW - REGIME OPTIMIZATION

KW - COLD ATMOSPHERIC PLASMA JET

KW - PENETRATION OF GOLD NANOPARTICLES

KW - PULSED VOLTAGE

KW - PULSE DURATION

KW - regime optimization

KW - penetration of gold nanoparticles

KW - pulsed voltage

KW - cold atmospheric plasma jet

KW - pulse duration

UR - https://www.elibrary.ru/item.asp?id=79631699

UR - https://www.mendeley.com/catalogue/0ff66991-b521-3c9a-a49f-904cbf54e6fd/

U2 - 10.1134/S1063780X24601597

DO - 10.1134/S1063780X24601597

M3 - Article

VL - 50

SP - 1375

EP - 1387

JO - Plasma Physics Reports

JF - Plasma Physics Reports

SN - 1063-780X

IS - 11

ER -