TY - JOUR

T1 - Effects of in vivo Administration of Insulin-Like Growth Factor-1 on Oocyte and Embryo Development in Mice under Chronic Psychosocial Stress

AU - Igonina, T. n.

AU - Lebedeva, D. a.

AU - Shavshaeva, N. a.

AU - Brusentsev, E. yu.

AU - Levinson, A. l.

AU - Amstislavsky, S. ya.

N1 - This work was supported by the Russian Science Foundation (project no. 23-25-00139).

PY - 2024/9/1

Y1 - 2024/9/1

N2 - Chronic stress may affect folliculogenesis. Understanding the relationship between stress and insulin-like growth factor-1 (IGF-1) is essential for addressing fertility issues. This study aimed to investigate the effects of in vivo IGF-1 administration on oocyte and embryo development in mice in conditions of chronic psychosocial stress. Female CD1 mice were subjected to alternating periods of social isolation and overcrowding. The stress response was confirmed by elevated corticosterone levels in blood serum. There were no significant differences in the number of ovulated oocytes across the groups. However, stress reduced oocyte maturation, while IGF-1 treatment of stressed females improved oocyte maturation and reduced their degraded rate. Stressed females exhibited a reduction in mitochondrial membrane potential in oocytes. IGF-1 treatment improved oocyte quality, increased mitochondrial potential, and reduced ROS levels in the oocytes collected from stressed female mice. There were no significant changes in apoptosis levels within cumulus-oocyte complexes or preimplantation embryos across all the studied groups. IGF-1 treatment in stressed females led to a higher number of cells in embryos compared to embryos collected from non-stressed controls and from non IGF-1 treated stressed females. In conclusion, chronic psychosocial stress negatively affects oocyte development and mitochondrial function in particular, but IGF-1 treatment mitigates these effects, improving oocyte and embryo quality. These results suggest a promising therapeutic role for IGF-1 in enhancing reproductive outcomes under stress conditions.

AB - Chronic stress may affect folliculogenesis. Understanding the relationship between stress and insulin-like growth factor-1 (IGF-1) is essential for addressing fertility issues. This study aimed to investigate the effects of in vivo IGF-1 administration on oocyte and embryo development in mice in conditions of chronic psychosocial stress. Female CD1 mice were subjected to alternating periods of social isolation and overcrowding. The stress response was confirmed by elevated corticosterone levels in blood serum. There were no significant differences in the number of ovulated oocytes across the groups. However, stress reduced oocyte maturation, while IGF-1 treatment of stressed females improved oocyte maturation and reduced their degraded rate. Stressed females exhibited a reduction in mitochondrial membrane potential in oocytes. IGF-1 treatment improved oocyte quality, increased mitochondrial potential, and reduced ROS levels in the oocytes collected from stressed female mice. There were no significant changes in apoptosis levels within cumulus-oocyte complexes or preimplantation embryos across all the studied groups. IGF-1 treatment in stressed females led to a higher number of cells in embryos compared to embryos collected from non-stressed controls and from non IGF-1 treated stressed females. In conclusion, chronic psychosocial stress negatively affects oocyte development and mitochondrial function in particular, but IGF-1 treatment mitigates these effects, improving oocyte and embryo quality. These results suggest a promising therapeutic role for IGF-1 in enhancing reproductive outcomes under stress conditions.

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001347081300008

U2 - 10.1134/S0022093024050065

DO - 10.1134/S0022093024050065

M3 - Article

VL - 60

SP - 1725

EP - 1740

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 5

ER -