TY - JOUR

T1 - Effects of Assisted Reproductive Technologies on Social Behavior of BTBR Mice, a Model of Autism Spectrum Disorders

AU - Rozhkova, I. N.

AU - Okotrub, S. V.

AU - Brusentsev, E. Yu.

AU - Igonina, T. N.

AU - Rakhmanova, T. A.

AU - Lebedeva, D. A.

AU - Yatsuk, T. A.

AU - Kozeneva, V. S.

AU - Naprimerov, V. A.

AU - Amstislavsky, S. Ya.

N1 - Effects of Assisted Reproductive Technologies on Social Behavior of BTBR Mice, a Model of Autism Spectrum Disorders / I. N. Rozhkova, S. V. Okotrub, E. Yu. Brusentsev [et al.] // Journal of Evolutionary Biochemistry and Physiology. – 2023. – Vol. 59, No. 2. – P. 458-472. – DOI 10.1134/s0022093023020138. This work was partially funded from the budgetary project no. FWNR-2022-0002 of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences (ICG SB RAS) and the Russian Foundation for Basic Research (RFBR grant no. 20-015-00162). The studies were carried out using the equipment of the Center for Genetic Resources of Laboratory Animals at the ICG SB RAS, supported by the Ministry of Education and Science of the Russian Federation (Unique project identifier RFMEFI62119X0023).

PY - 2023/4/26

Y1 - 2023/4/26

N2 - This work is the first attempt to study the effect of suchan assisted reproductive technology (ART) as in vitro culture ofpreimplantation embryos on social behavior of offspring, using BTBR (BTBRT+Itpr3tf/J) mice as an idiopathic model of autism. C57BL/6J micewere used as controls. Social behavior was investigated in the adultoffspring of mice obtained either by natural conception (C57BL/6Jand BTBR groups) or after in vitro culture and embryo transfer (ET)of appropriate mouse strains to pseudopregnant C57BL/6J females(ET-C57BL/6J and ET-BTBR groups). The BTBR mice demonstrated reducedlevels of social recognition and motivation compared to C57BL/6Jmice. However, ET-C57BL/6J and ET-BTBR females demonstrated a decreasedinterest in a novel partner (stranger) compared to naturally conceivedcontrols of the same sex, as revealed in a social recognition sessionof this test. Thus, in vitro culture of embryo affects social recognitionin the offspring of C57BL/6J and BTBR mice, but in females only.

AB - This work is the first attempt to study the effect of suchan assisted reproductive technology (ART) as in vitro culture ofpreimplantation embryos on social behavior of offspring, using BTBR (BTBRT+Itpr3tf/J) mice as an idiopathic model of autism. C57BL/6J micewere used as controls. Social behavior was investigated in the adultoffspring of mice obtained either by natural conception (C57BL/6Jand BTBR groups) or after in vitro culture and embryo transfer (ET)of appropriate mouse strains to pseudopregnant C57BL/6J females(ET-C57BL/6J and ET-BTBR groups). The BTBR mice demonstrated reducedlevels of social recognition and motivation compared to C57BL/6Jmice. However, ET-C57BL/6J and ET-BTBR females demonstrated a decreasedinterest in a novel partner (stranger) compared to naturally conceivedcontrols of the same sex, as revealed in a social recognition sessionof this test. Thus, in vitro culture of embryo affects social recognitionin the offspring of C57BL/6J and BTBR mice, but in females only.

KW - C57BL/6J

KW - BTBR

KW - PREIMPLANTATION EMBRYOS

KW - ASSISTED REPRODUCTIVE TECHNOLOGIES

KW - IN VITRO CULTURE

KW - AUTISM SPECTRUM DISORDERS

KW - SOCIAL BEHAVIOR

KW - EVOLUTIONARY BIOLOGY

KW - BIOCHEMISTRY

KW - GENERAL

KW - ANIMAL PHYSIOLOGY

UR - https://www.mendeley.com/catalogue/fd4d1eac-71ef-3a3b-8ef8-e64cc2c81512/

UR - https://www.elibrary.ru/item.asp?id=61498200

U2 - 10.1134/s0022093023020138

DO - 10.1134/s0022093023020138

M3 - Article

VL - 59

SP - 458

EP - 472

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 2

ER -