Effectiveness of Repeated Administration of Praziquantel with Disodium Glycyrrhizinate and Two Enantiomers of Praziquantel on Opisthorchis felineus (Rivolta, 1884)

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Effectiveness of Repeated Administration of Praziquantel with Disodium Glycyrrhizinate and Two Enantiomers of Praziquantel on Opisthorchis felineus (Rivolta, 1884). / Avgustinovich, D. F.; Tsyganov, M. A.; Pakharukova, M. Y. et al.

In: Acta Parasitologica, Vol. 65, No. 1, 01.03.2020, p. 156-164.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{8db8ff337e42420596b976d6b14013fb,

title = "Effectiveness of Repeated Administration of Praziquantel with Disodium Glycyrrhizinate and Two Enantiomers of Praziquantel on Opisthorchis felineus (Rivolta, 1884)",

abstract = "Background: Nowadays, it is still important to develop effective anti-opisthorchiasis agents. In this work, we tested a complex of praziquantel (PZQ) with a plant origin compound—disodium glycyrrhizinate—in the ratio 1:10 PZQ:Na2GA, containing 11-fold less of the active ingredient. Our aim was to study various ways to treat trematode Opisthorchis felineus with this complex in vitro. Additionally, an in vitro comparison of the anthelmintic action was made among racemic-PZQ, (R)-PZQ, and (S)-PZQ on juvenile and adult maritae of O. felineus. Methods: Worms extracted from the hamsters were subjected to various regimens of administration of the complex: once a day for 3 days or three times within 1 day. Moreover, mature maritae and juvenile worms of O. felineus were subjected to the comparison the anthelmintic effectiveness of racemic-PZQ, (R)-PZQ, and (S)-PZQ. Results: The O. felineus maritae that received PZQ:Na2GA (1:10) thrice within 1 day were most strongly affected by the drug. Their motility substantially decreased already on the second day after the last dose, and the percentage of live worms by the end of the experimental period was the lowest. These results indicate a cumulative anthelmintic effect of this substance under the regimen “three times within 1 day.” For the first time, we report that among the three substances (racemic-PZQ and two enantiomers), (R)-PZQ has the highest anthelmintic activity, toward both juvenile and sexually mature maritae of O. felineus. Conclusion: These findings suggest that the development of a supramolecular complex of (R)-PZQ with disodium glycyrrhizinate and administration of this complex three times within 1 day are promising approaches.",

keywords = "(R)-PZQ and (S)-PZQ, Disodium glycyrrhizinate, Opisthorchis felineus, Praziquantel, VIVERRINI, INFECTIONS, ACID, DRUGS",

author = "Avgustinovich, {D. F.} and Tsyganov, {M. A.} and Pakharukova, {M. Y.} and Chulakov, {E. N.} and Dushkin, {A. V.} and Krasnov, {V. P.} and Mordvinov, {V. A.}",

year = "2020",

month = mar,

day = "1",

doi = "10.2478/s11686-019-00149-2",

language = "English",

volume = "65",

pages = "156--164",

journal = "Acta Parasitologica",

issn = "1230-2821",

publisher = "Walter de Gruyter GmbH",

number = "1",

}

RIS

TY - JOUR

T1 - Effectiveness of Repeated Administration of Praziquantel with Disodium Glycyrrhizinate and Two Enantiomers of Praziquantel on Opisthorchis felineus (Rivolta, 1884)

AU - Avgustinovich, D. F.

AU - Tsyganov, M. A.

AU - Pakharukova, M. Y.

AU - Chulakov, E. N.

AU - Dushkin, A. V.

AU - Krasnov, V. P.

AU - Mordvinov, V. A.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - Background: Nowadays, it is still important to develop effective anti-opisthorchiasis agents. In this work, we tested a complex of praziquantel (PZQ) with a plant origin compound—disodium glycyrrhizinate—in the ratio 1:10 PZQ:Na2GA, containing 11-fold less of the active ingredient. Our aim was to study various ways to treat trematode Opisthorchis felineus with this complex in vitro. Additionally, an in vitro comparison of the anthelmintic action was made among racemic-PZQ, (R)-PZQ, and (S)-PZQ on juvenile and adult maritae of O. felineus. Methods: Worms extracted from the hamsters were subjected to various regimens of administration of the complex: once a day for 3 days or three times within 1 day. Moreover, mature maritae and juvenile worms of O. felineus were subjected to the comparison the anthelmintic effectiveness of racemic-PZQ, (R)-PZQ, and (S)-PZQ. Results: The O. felineus maritae that received PZQ:Na2GA (1:10) thrice within 1 day were most strongly affected by the drug. Their motility substantially decreased already on the second day after the last dose, and the percentage of live worms by the end of the experimental period was the lowest. These results indicate a cumulative anthelmintic effect of this substance under the regimen “three times within 1 day.” For the first time, we report that among the three substances (racemic-PZQ and two enantiomers), (R)-PZQ has the highest anthelmintic activity, toward both juvenile and sexually mature maritae of O. felineus. Conclusion: These findings suggest that the development of a supramolecular complex of (R)-PZQ with disodium glycyrrhizinate and administration of this complex three times within 1 day are promising approaches.

AB - Background: Nowadays, it is still important to develop effective anti-opisthorchiasis agents. In this work, we tested a complex of praziquantel (PZQ) with a plant origin compound—disodium glycyrrhizinate—in the ratio 1:10 PZQ:Na2GA, containing 11-fold less of the active ingredient. Our aim was to study various ways to treat trematode Opisthorchis felineus with this complex in vitro. Additionally, an in vitro comparison of the anthelmintic action was made among racemic-PZQ, (R)-PZQ, and (S)-PZQ on juvenile and adult maritae of O. felineus. Methods: Worms extracted from the hamsters were subjected to various regimens of administration of the complex: once a day for 3 days or three times within 1 day. Moreover, mature maritae and juvenile worms of O. felineus were subjected to the comparison the anthelmintic effectiveness of racemic-PZQ, (R)-PZQ, and (S)-PZQ. Results: The O. felineus maritae that received PZQ:Na2GA (1:10) thrice within 1 day were most strongly affected by the drug. Their motility substantially decreased already on the second day after the last dose, and the percentage of live worms by the end of the experimental period was the lowest. These results indicate a cumulative anthelmintic effect of this substance under the regimen “three times within 1 day.” For the first time, we report that among the three substances (racemic-PZQ and two enantiomers), (R)-PZQ has the highest anthelmintic activity, toward both juvenile and sexually mature maritae of O. felineus. Conclusion: These findings suggest that the development of a supramolecular complex of (R)-PZQ with disodium glycyrrhizinate and administration of this complex three times within 1 day are promising approaches.

KW - (R)-PZQ and (S)-PZQ

KW - Disodium glycyrrhizinate

KW - Opisthorchis felineus

KW - Praziquantel

KW - VIVERRINI

KW - INFECTIONS

KW - ACID

KW - DRUGS

UR - http://www.scopus.com/inward/record.url?scp=85076041688&partnerID=8YFLogxK

U2 - 10.2478/s11686-019-00149-2

DO - 10.2478/s11686-019-00149-2

M3 - Article

C2 - 31797191

AN - SCOPUS:85076041688

VL - 65

SP - 156

EP - 164

JO - Acta Parasitologica

JF - Acta Parasitologica

SN - 1230-2821

IS - 1

ER -

ID: 22547214