TY - JOUR

T1 - Effect of thidiazuron and meta-topolin on in vitro shoot multiplication and development of the Populus davidiana × P. bolleana hybrid

AU - Zheleznichenko, Tatiana

AU - Karakulov, Anatoly

AU - Asbaganov, Sergey

AU - Kochetov, Alex

N1 - This research was conducted within the framework of the Ministry of Science and Higher Education grant to the Institute of Cytology and Genetics, SB RAS (No. FWNR-2022-0017). This work was also supported in the frame the state assignments to Central Siberian Botanical Garden, SB RAS: No. АААА-А21-121011290025-2 (as a part of project “Analysis of biodiversity, conservation and restoration of rare and economically valuable plant species using experimental methods”) and No. АААА-А21-121011290027-6 (as a part of project “Theoretical and applied aspects of studying the gene pools of natural plant populations and conserving plant diversity ex situ”). This study utilized materials from the Central Siberian Botanical Garden SB RAS collection: unique scientific unit (USU) No. 440534 “Collection of living plants indoors and outdoors.” Effect of thidiazuron and meta-topolin on in vitro shoot multiplication and development of the Populus davidiana × P. bolleana hybrid / T. Zheleznichenko, A. Karakulov, S. Asbaganov, A. Kochetov // Plant Cell, Tissue and Organ Culture. - 2025. - Т. 163. № 1. - С. 35.

PY - 2025/10/14

Y1 - 2025/10/14

N2 - Populus davidiana × P. bolleana cv. Baicheng-1 is a female hybrid distinguished by seed production without fluff, exhibits hypoallergenic traits, rendering it valuable for mass propagation. Regeneration of the poplar under the influence of thidiazuron (TDZ) and meta-Topolin (m-T) at concentrations of 0.1, 0.5, and 1 µM on Murashige and Skoog (MS) medium from leaves and single-node segments of aseptic shoots was first studied. Direct organogenesis was observed with all types and concentrations of phytohormones. Nodal segments produced both axillary and adventitious shoots, while leaf explants generated only adventitious shoots under TDZ treatment. The number of shoots increased with higher TDZ concentrations, yielding 13.25 ± 3.0 to 26.16 ± 4.1 shoots per explant (3.4 to 4.4 times higher than m-T), but shoot height decreased from 12.54 ± 4.8 mm to ≥ 5 mm. m-T treatments produced more robust, and greener shoots with larger leaf blade areas. Rooting of all shoots reached 100% on hormone-free MS medium. Regenerants exhibited acclimatization survival rates of 84.7% − 100%. Preliminary assessment of clonal fidelity, using RAPD and ISSR markers, revealed an average genetic polymorphism of 58.4% between regenerants and the mother plant, and 34.4% among regenerants. The lowest variation observed in m-T-derived regenerants. Thus, TDZ proved the most effective for micropropagating, but m-T produces more viable and clonal fidelity regenerants. Nodal segments were the optimal explant type. An efficient system was developed for multiplication of P. davidiana × P. bolleana cv. Baicheng-1, which are hypoallergenic, ornamental, promising for urban landscaping. However, further studies of the genetic fidelity of the regenerants is necessary.

AB - Populus davidiana × P. bolleana cv. Baicheng-1 is a female hybrid distinguished by seed production without fluff, exhibits hypoallergenic traits, rendering it valuable for mass propagation. Regeneration of the poplar under the influence of thidiazuron (TDZ) and meta-Topolin (m-T) at concentrations of 0.1, 0.5, and 1 µM on Murashige and Skoog (MS) medium from leaves and single-node segments of aseptic shoots was first studied. Direct organogenesis was observed with all types and concentrations of phytohormones. Nodal segments produced both axillary and adventitious shoots, while leaf explants generated only adventitious shoots under TDZ treatment. The number of shoots increased with higher TDZ concentrations, yielding 13.25 ± 3.0 to 26.16 ± 4.1 shoots per explant (3.4 to 4.4 times higher than m-T), but shoot height decreased from 12.54 ± 4.8 mm to ≥ 5 mm. m-T treatments produced more robust, and greener shoots with larger leaf blade areas. Rooting of all shoots reached 100% on hormone-free MS medium. Regenerants exhibited acclimatization survival rates of 84.7% − 100%. Preliminary assessment of clonal fidelity, using RAPD and ISSR markers, revealed an average genetic polymorphism of 58.4% between regenerants and the mother plant, and 34.4% among regenerants. The lowest variation observed in m-T-derived regenerants. Thus, TDZ proved the most effective for micropropagating, but m-T produces more viable and clonal fidelity regenerants. Nodal segments were the optimal explant type. An efficient system was developed for multiplication of P. davidiana × P. bolleana cv. Baicheng-1, which are hypoallergenic, ornamental, promising for urban landscaping. However, further studies of the genetic fidelity of the regenerants is necessary.

KW - In vitro propagation

KW - P. davidiana × P. bolleana cv. baicheng-1

KW - RAPD and ISSR markers

KW - Thidiazuron

KW - meta-topolin

UR - https://www.mendeley.com/catalogue/19cb4f03-988f-33ac-bf45-baa76861661e/

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105019057171&origin=inward

U2 - 10.1007/s11240-025-03228-7

DO - 10.1007/s11240-025-03228-7

M3 - Article

VL - 163

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 1573-5044

IS - 1

M1 - 35

ER -