Research output: Contribution to journal › Article › peer-review
Effect of low temperatures on cytochrome photoresponse in mouse embryos. / Sazhina, E. A.; Okotrub, K. A.; Amstislavsky, S. Y. et al.
In: Archives of Biochemistry and Biophysics, Vol. 669, 15.07.2019, p. 32-38.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effect of low temperatures on cytochrome photoresponse in mouse embryos
AU - Sazhina, E. A.
AU - Okotrub, K. A.
AU - Amstislavsky, S. Y.
AU - Surovtsev, N. V.
N1 - Copyright © 2019 Elsevier Inc. All rights reserved.
PY - 2019/7/15
Y1 - 2019/7/15
N2 - Embryos cryopreservation is a widely used technology for genetic resources storage. Cryopreservation suppresses cell respiration, but very little is known about the changes that occur with mitochondria at low temperatures. We used Raman spectroscopy to investigate photoresponse and redox state of cytochromes in the respiratory electron transport chain (ETC)in early mouse embryos during cooling. Redox state of cytochromes was probed by the intensity of cytochrome resonance Raman lines. Photoinduced reactions of cytochromes were used to study the changes in the rates of redox reactions. It is found that the rate of cytochrome photoresponse detected by Raman spectra abruptly changes when embryos are cooled below −50 °C. Raman mapping revealed that the average intensity of cytochrome Raman peaks at −65 °C is higher than at −40 °C. Cytochrome b reduction was found in embryos frozen below −50 °C when irradiated with 532 nm laser radiation. These effects were observed for cells frozen in aqueous solutions of two different cryoprotectants: glycerol and propylene glycol. Raman spectroscopy of cytochromes reveals the abrupt changes in the ETC work of frozen mouse embryos at temperatures below −50 °C. We suggest that similar phenomena can be found in various cell types.
AB - Embryos cryopreservation is a widely used technology for genetic resources storage. Cryopreservation suppresses cell respiration, but very little is known about the changes that occur with mitochondria at low temperatures. We used Raman spectroscopy to investigate photoresponse and redox state of cytochromes in the respiratory electron transport chain (ETC)in early mouse embryos during cooling. Redox state of cytochromes was probed by the intensity of cytochrome resonance Raman lines. Photoinduced reactions of cytochromes were used to study the changes in the rates of redox reactions. It is found that the rate of cytochrome photoresponse detected by Raman spectra abruptly changes when embryos are cooled below −50 °C. Raman mapping revealed that the average intensity of cytochrome Raman peaks at −65 °C is higher than at −40 °C. Cytochrome b reduction was found in embryos frozen below −50 °C when irradiated with 532 nm laser radiation. These effects were observed for cells frozen in aqueous solutions of two different cryoprotectants: glycerol and propylene glycol. Raman spectroscopy of cytochromes reveals the abrupt changes in the ETC work of frozen mouse embryos at temperatures below −50 °C. We suggest that similar phenomena can be found in various cell types.
KW - Cryopreservation
KW - Cytochrome
KW - Glycerol
KW - Mouse embryo
KW - Propane-1,2-diol
KW - Raman spectroscopy
KW - OXIDATION
KW - REDOX STATE
KW - LIGHT
KW - RESONANCE RAMAN-SPECTRA
KW - CRYOPRESERVATION
KW - MITOCHONDRIAL-MEMBRANE
KW - INTRACELLULAR ICE FORMATION
KW - PHASE-TRANSITION
KW - FROZEN
KW - CELL
UR - http://www.scopus.com/inward/record.url?scp=85066254649&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2019.05.017
DO - 10.1016/j.abb.2019.05.017
M3 - Article
C2 - 31128967
AN - SCOPUS:85066254649
VL - 669
SP - 32
EP - 38
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
ER -
ID: 20346475