Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe. / Glazachev, Yuri I.; Schlotgauer, Anna A.; Timoshnikov, Viktor A. et al.
In: Journal of Membrane Biology, Vol. 253, No. 4, 01.08.2020, p. 343-356.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effect of Glycyrrhizic Acid and Arabinogalactan on the Membrane Potential of Rat Thymocytes Studied by Potential-Sensitive Fluorescent Probe
AU - Glazachev, Yuri I.
AU - Schlotgauer, Anna A.
AU - Timoshnikov, Viktor A.
AU - Kononova, Polina A.
AU - Selyutina, Olga Yu
AU - Shelepova, Ekaterina A.
AU - Zelikman, Maxim V.
AU - Khvostov, Mikhail V.
AU - Polyakov, Nikolay E.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micellar form resulted in a decrease of the amplitude of observed fluorescence kinetics that points out to a decrease of the transmembrane potential. The proposed mechanism is an increase of membrane ion permeability (passive ion transport) of the plasma cell membrane due to GA incorporation. The incorporation of GA molecules into the cell membrane is extremely sensitive to the degree of GA dissociation. The neutral form of glycyrrhizic acid enters the lipid bilayer in contrast to the deprotonated anionic form. The incubation of rat thymocytes with anionic form of GA, namely with its disodium salt, has no effect on the fluorescence kinetics. The possible reasons of this phenomenon are discussed in the light of the nuclear magnetic resonance (NMR) and molecular dynamics (MD) data. The treatment of thymocytes with AG affects only the initial rate of the probe incorporation. The proposed mechanism is that AG covers the surface of the cell membrane and forms a barrier for the probe. Additionally, our experiments demonstrated that both polysaccharide AG and GA in the neutral form (but not Na 2GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane.
AB - The effect of the natural saponin glycyrrhizic acid (GA) and polysaccharide arabinogalactan (AG) on the transmembrane potential of rat thymocytes was investigated using the potential-sensitive fluorescent probe 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM). Incubation of cells with GA in micellar form resulted in a decrease of the amplitude of observed fluorescence kinetics that points out to a decrease of the transmembrane potential. The proposed mechanism is an increase of membrane ion permeability (passive ion transport) of the plasma cell membrane due to GA incorporation. The incorporation of GA molecules into the cell membrane is extremely sensitive to the degree of GA dissociation. The neutral form of glycyrrhizic acid enters the lipid bilayer in contrast to the deprotonated anionic form. The incubation of rat thymocytes with anionic form of GA, namely with its disodium salt, has no effect on the fluorescence kinetics. The possible reasons of this phenomenon are discussed in the light of the nuclear magnetic resonance (NMR) and molecular dynamics (MD) data. The treatment of thymocytes with AG affects only the initial rate of the probe incorporation. The proposed mechanism is that AG covers the surface of the cell membrane and forms a barrier for the probe. Additionally, our experiments demonstrated that both polysaccharide AG and GA in the neutral form (but not Na 2GA) effectively capture the cationic probe in an aqueous solution and then deliver it to the cell membrane.
KW - Arabinogalactan
KW - Drug delivery
KW - Glycyrrhizic acid
KW - Membrane potential
KW - Thymocytes
KW - DRUG
KW - CELLS
KW - OLIGOSACCHARIDES
KW - TRANSDERMAL DELIVERY
KW - BIOAVAILABILITY
KW - PERMEABILITY TRANSITION
KW - BETA-CYCLODEXTRIN
KW - DYNAMICS
KW - FORCE-FIELD
KW - POLYSACCHARIDE ARABINOGALACTAN
UR - http://www.scopus.com/inward/record.url?scp=85088957806&partnerID=8YFLogxK
U2 - 10.1007/s00232-020-00132-3
DO - 10.1007/s00232-020-00132-3
M3 - Article
C2 - 32725429
AN - SCOPUS:85088957806
VL - 253
SP - 343
EP - 356
JO - Journal of Membrane Biology
JF - Journal of Membrane Biology
SN - 0022-2631
IS - 4
ER -
ID: 24961043