TY - JOUR

T1 - Ecological safety of insecticide based on entomopathogenic virus DsCPV-1 for nontarget invertebrates

AU - Belevitch, Olga

AU - Yurchenko, Yuri

AU - Kharlamova, Daria

AU - Shatalova, Elena

AU - Agrikolyanskaya, Natalia

AU - Subbotina, Anna

AU - Ignatieva, Anastasia

AU - Tokarev, Yuri

AU - Martemyanov, Vyacheslav

N1 - The study was supported by Russian Science Foundation grants # 23-66-10015 (molecular studies) and #23-16-00262 (bioassays with terrestrial species), as well as Federal Fundamental Scientific Research Program for 2021\u20132025 #FWGS-2021-0001 (bioassays with hydrobionts).

PY - 2024/12

Y1 - 2024/12

N2 - For the first time under laboratory conditions, the virulence of a unique cypovirus strain, DsCPV-1, which has broad host specificity, was tested on nontarget aquatic organisms (natural species: Gammarus lacustris, Anopheles messeae, Coenagrion lunulatum, Cloeon robusta, Chironomus sp., Ilyocoris cimicoides, and Plea minutissima; laboratory species: Aedes aegypti and Daphnia magna), a terrestrial pollinator species (Apis mellifera), and an entomophage (Podisus maculiventris). The probability of this virus’s accumulation in the bodies of invertebrates and of its transmission along a trophic chain was evaluated by two approaches: bioassays and a molecular diagnostic analysis. In the bioassays, there was no significant increase in mortality among all the tested aquatic and terrestrial nontarget species exposed to DsCPV-1 as compared with control groups (no virus). When we fed Podisus maculiventris with caterpillars having active DsCPV-1 infection (i.e., with the virus replicating in the host) no viral replication was observed in bug. No replication was also observed in mosquitos as well as in bee after viral treatment. Thus, the results show that the DsCPV-1 virus has excellent environmental safety toward many invertebrate species and can be recommended for the control of lepidopteran pests in forestry and agriculture as insecticide with light effect on environment.

AB - For the first time under laboratory conditions, the virulence of a unique cypovirus strain, DsCPV-1, which has broad host specificity, was tested on nontarget aquatic organisms (natural species: Gammarus lacustris, Anopheles messeae, Coenagrion lunulatum, Cloeon robusta, Chironomus sp., Ilyocoris cimicoides, and Plea minutissima; laboratory species: Aedes aegypti and Daphnia magna), a terrestrial pollinator species (Apis mellifera), and an entomophage (Podisus maculiventris). The probability of this virus’s accumulation in the bodies of invertebrates and of its transmission along a trophic chain was evaluated by two approaches: bioassays and a molecular diagnostic analysis. In the bioassays, there was no significant increase in mortality among all the tested aquatic and terrestrial nontarget species exposed to DsCPV-1 as compared with control groups (no virus). When we fed Podisus maculiventris with caterpillars having active DsCPV-1 infection (i.e., with the virus replicating in the host) no viral replication was observed in bug. No replication was also observed in mosquitos as well as in bee after viral treatment. Thus, the results show that the DsCPV-1 virus has excellent environmental safety toward many invertebrate species and can be recommended for the control of lepidopteran pests in forestry and agriculture as insecticide with light effect on environment.

KW - Ecologically friendly bioinsecticide

KW - Entomophage

KW - Green technology

KW - Honey bee

KW - Hydrobiont

KW - Pollinator

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85210096554&origin=inward&txGid=d5527fb8a1557438d0c9ab93c6fc0fde

UR - https://www.mendeley.com/catalogue/bcb9e82f-78df-37fd-a4d7-e1a35af9b245/

U2 - 10.1038/s41598-024-78471-7

DO - 10.1038/s41598-024-78471-7

M3 - Article

C2 - 39582042

VL - 14

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 29093

ER -