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Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context. / Ovcherenko, Sergey S; Shernyukov, Andrey V; Nasonov, Dmitry M et al.

In: Journal of the American Chemical Society, Vol. 145, No. 10, 15.03.2023, p. 5613-5617.

Research output: Contribution to journal › Article › peer-review

Harvard

Ovcherenko, SS, Shernyukov, AV, Nasonov, DM, Endutkin, AV, Zharkov, DO & Bagryanskaya, EG 2023, 'Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context', Journal of the American Chemical Society, vol. 145, no. 10, pp. 5613-5617. https://doi.org/10.1021/jacs.2c11230

APA

Ovcherenko, S. S., Shernyukov, A. V., Nasonov, D. M., Endutkin, A. V., Zharkov, D. O., & Bagryanskaya, E. G. (2023). Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context. Journal of the American Chemical Society, 145(10), 5613-5617. https://doi.org/10.1021/jacs.2c11230

Vancouver

Ovcherenko SS, Shernyukov AV, Nasonov DM, Endutkin AV, Zharkov DO, Bagryanskaya EG. Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context. Journal of the American Chemical Society. 2023 Mar 15;145(10):5613-5617. Epub 2023 Mar 2. doi: 10.1021/jacs.2c11230

Author

Ovcherenko, Sergey S ; Shernyukov, Andrey V ; Nasonov, Dmitry M et al. / Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context. In: Journal of the American Chemical Society. 2023 ; Vol. 145, No. 10. pp. 5613-5617.

BibTeX

@article{4b8f013411bc4f1681dfb51bd3eca20b,
title = "Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context",
abstract = "8-Oxo-7,8-dihydroguanine (oxoG), an abundant DNA lesion, can mispair with adenine and induce mutations. To prevent this, cells possess DNA repair glycosylases that excise either oxoG from oxoG:C pairs (bacterial Fpg, human OGG1) or A from oxoG:A mispairs (bacterial MutY, human MUTYH). Early lesion recognition steps remain murky and may include enforced base pair opening or capture of a spontaneously opened pair. We adapted the CLEANEX-PM NMR protocol to detect DNA imino proton exchange and analyzed the dynamics of oxoG:C, oxoG:A, and their undamaged counterparts in nucleotide contexts with different stacking energy. Even in a poorly stacking context, the oxoG:C pair did not open easier than G:C, arguing against extrahelical base capture by Fpg/OGG1. On the contrary, oxoG opposite A significantly populated the extrahelical state, which may assist recognition by MutY/MUTYH.",
keywords = "Base Pairing, DNA Repair, DNA/chemistry, Guanine/chemistry, Humans, Nucleotides",
author = "Ovcherenko, {Sergey S} and Shernyukov, {Andrey V} and Nasonov, {Dmitry M} and Endutkin, {Anton V} and Zharkov, {Dmitry O} and Bagryanskaya, {Elena G}",
note = "Funding: This study was supported by Russian Science Foundation (21-14-00219). Partial salary support from Russian Ministry of Science and Higher Education (12103130005-8) is acknowledged.",
year = "2023",
month = mar,
day = "15",
doi = "10.1021/jacs.2c11230",
language = "English",
volume = "145",
pages = "5613--5617",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "10",

}

RIS

TY - JOUR

T1 - Dynamics of 8-Oxoguanine in DNA: Decisive Effects of Base Pairing and Nucleotide Context

AU - Ovcherenko, Sergey S

AU - Shernyukov, Andrey V

AU - Nasonov, Dmitry M

AU - Endutkin, Anton V

AU - Zharkov, Dmitry O

AU - Bagryanskaya, Elena G

N1 - Funding: This study was supported by Russian Science Foundation (21-14-00219). Partial salary support from Russian Ministry of Science and Higher Education (12103130005-8) is acknowledged.

PY - 2023/3/15

Y1 - 2023/3/15

N2 - 8-Oxo-7,8-dihydroguanine (oxoG), an abundant DNA lesion, can mispair with adenine and induce mutations. To prevent this, cells possess DNA repair glycosylases that excise either oxoG from oxoG:C pairs (bacterial Fpg, human OGG1) or A from oxoG:A mispairs (bacterial MutY, human MUTYH). Early lesion recognition steps remain murky and may include enforced base pair opening or capture of a spontaneously opened pair. We adapted the CLEANEX-PM NMR protocol to detect DNA imino proton exchange and analyzed the dynamics of oxoG:C, oxoG:A, and their undamaged counterparts in nucleotide contexts with different stacking energy. Even in a poorly stacking context, the oxoG:C pair did not open easier than G:C, arguing against extrahelical base capture by Fpg/OGG1. On the contrary, oxoG opposite A significantly populated the extrahelical state, which may assist recognition by MutY/MUTYH.

AB - 8-Oxo-7,8-dihydroguanine (oxoG), an abundant DNA lesion, can mispair with adenine and induce mutations. To prevent this, cells possess DNA repair glycosylases that excise either oxoG from oxoG:C pairs (bacterial Fpg, human OGG1) or A from oxoG:A mispairs (bacterial MutY, human MUTYH). Early lesion recognition steps remain murky and may include enforced base pair opening or capture of a spontaneously opened pair. We adapted the CLEANEX-PM NMR protocol to detect DNA imino proton exchange and analyzed the dynamics of oxoG:C, oxoG:A, and their undamaged counterparts in nucleotide contexts with different stacking energy. Even in a poorly stacking context, the oxoG:C pair did not open easier than G:C, arguing against extrahelical base capture by Fpg/OGG1. On the contrary, oxoG opposite A significantly populated the extrahelical state, which may assist recognition by MutY/MUTYH.

KW - Base Pairing

KW - DNA Repair

KW - DNA/chemistry

KW - Guanine/chemistry

KW - Humans

KW - Nucleotides

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85149386454&origin=inward&txGid=b7831bc74bd52444d36f9da89fa8ebb0

UR - https://www.mendeley.com/catalogue/29550847-2d17-31f9-8119-491677197e75/

U2 - 10.1021/jacs.2c11230

DO - 10.1021/jacs.2c11230

M3 - Article

C2 - 36867834

VL - 145

SP - 5613

EP - 5617

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 10

ER -

ID: 44922214