Research output: Contribution to journal › Article › peer-review
Drosophila female fertility and juvenile hormone metabolism depends on the type of Wolbachia infection. / Gruntenko, Nataly E.; Karpova, Evgenia K.; Adonyeva, Natalya V. et al.
In: The Journal of experimental biology, Vol. 222, No. Pt 4, jeb195347, 22.02.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Drosophila female fertility and juvenile hormone metabolism depends on the type of Wolbachia infection
AU - Gruntenko, Nataly E.
AU - Karpova, Evgenia K.
AU - Adonyeva, Natalya V.
AU - Andreenkova, Olga V.
AU - Burdina, Elena V.
AU - Ilinsky, Yury Yu
AU - Bykov, Roman A.
AU - Menshanov, Petr N.
AU - Rauschenbach, Inga Yu
N1 - © 2019. Published by The Company of Biologists Ltd.
PY - 2019/2/22
Y1 - 2019/2/22
N2 - Maternally inherited intracellular bacteria Wolbachia cause both parasitic and mutualistic effects on their numerous insect hosts, including manipulating the host reproductive system in order to increase the bacteria spreading in a host population, and increasing the host fitness. Here, we demonstrate that the type of Wolbachia infection determines the effect on Drosophila melanogaster egg production as a proxy for fecundity, and metabolism of juvenile hormone (JH), which acts as gonadotropin in adult insects. For this study, we used six D. melanogaster lineages carrying the nuclear background of interbred Bi90 lineage and cytoplasmic backgrounds with or without Wolbachia of different genotype variants. The wMelCS genotype of Wolbachia decreases egg production in infected D. melanogaster females in the beginning of oviposition and increases it later (from the sixth day after eclosion), whereas the wMelPop Wolbachia strain causes the opposite effect, and the wMel, wMel2 and wMel4 genotypes of Wolbachia do not show any effect on these traits compared with uninfected Bi90 D. melanogaster females. The intensity of JH catabolism negatively correlates with the fecundity level in the flies carrying both wMelCS and wMelPop Wolbachia The JH catabolism in females infected with genotypes of the wMel group does not differ from that in uninfected females. The effects of wMelCS and wMelPop infection on egg production can be levelled by the modulation of JH titre (via precocene/JH treatment of the flies). Thus, at least one of the mechanisms promoting the effect of Wolbachia on D. melanogaster female fecundity is mediated by JH.
AB - Maternally inherited intracellular bacteria Wolbachia cause both parasitic and mutualistic effects on their numerous insect hosts, including manipulating the host reproductive system in order to increase the bacteria spreading in a host population, and increasing the host fitness. Here, we demonstrate that the type of Wolbachia infection determines the effect on Drosophila melanogaster egg production as a proxy for fecundity, and metabolism of juvenile hormone (JH), which acts as gonadotropin in adult insects. For this study, we used six D. melanogaster lineages carrying the nuclear background of interbred Bi90 lineage and cytoplasmic backgrounds with or without Wolbachia of different genotype variants. The wMelCS genotype of Wolbachia decreases egg production in infected D. melanogaster females in the beginning of oviposition and increases it later (from the sixth day after eclosion), whereas the wMelPop Wolbachia strain causes the opposite effect, and the wMel, wMel2 and wMel4 genotypes of Wolbachia do not show any effect on these traits compared with uninfected Bi90 D. melanogaster females. The intensity of JH catabolism negatively correlates with the fecundity level in the flies carrying both wMelCS and wMelPop Wolbachia The JH catabolism in females infected with genotypes of the wMel group does not differ from that in uninfected females. The effects of wMelCS and wMelPop infection on egg production can be levelled by the modulation of JH titre (via precocene/JH treatment of the flies). Thus, at least one of the mechanisms promoting the effect of Wolbachia on D. melanogaster female fecundity is mediated by JH.
KW - Egg production
KW - Fecundity
KW - Heat stress
KW - Juvenile hormone
KW - Oocytes
KW - Precocene
KW - CYTOPLASMIC INCOMPATIBILITY
KW - STRESS-RESPONSE
KW - POPULATIONS
KW - RECEPTOR
KW - PIPIENTIS
KW - BACTERIAL ENDOSYMBIONTS
KW - PATHWAY
KW - BIOLOGY
UR - http://www.scopus.com/inward/record.url?scp=85062026660&partnerID=8YFLogxK
U2 - 10.1242/jeb.195347
DO - 10.1242/jeb.195347
M3 - Article
C2 - 30679245
AN - SCOPUS:85062026660
VL - 222
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
SN - 0022-0949
IS - Pt 4
M1 - jeb195347
ER -
ID: 18626911