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Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. / Chanyshev, Mikhail D.; Yarushkin, Andrey A.; Koldysheva, Elena V. et al.

In: Toxicology Mechanisms and Methods, Vol. 30, No. 8, 12.10.2020, p. 562-569.

Research output: Contribution to journal › Article › peer-review

Harvard

Chanyshev, MD, Yarushkin, AA, Koldysheva, EV, Lushnikova, EL & Gulyaeva, LF 2020, 'Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure', Toxicology Mechanisms and Methods, vol. 30, no. 8, pp. 562-569. https://doi.org/10.1080/15376516.2020.1777493

APA

Chanyshev, M. D., Yarushkin, A. A., Koldysheva, E. V., Lushnikova, E. L., & Gulyaeva, L. F. (2020). Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. Toxicology Mechanisms and Methods, 30(8), 562-569. https://doi.org/10.1080/15376516.2020.1777493

Vancouver

Chanyshev MD, Yarushkin AA, Koldysheva EV, Lushnikova EL, Gulyaeva LF. Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. Toxicology Mechanisms and Methods. 2020 Oct 12;30(8):562-569. Epub 2020 Jun 29. doi: 10.1080/15376516.2020.1777493

Author

Chanyshev, Mikhail D. ; Yarushkin, Andrey A. ; Koldysheva, Elena V. et al. / Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. In: Toxicology Mechanisms and Methods. 2020 ; Vol. 30, No. 8. pp. 562-569.

BibTeX

@article{9d9d98b68fad49de8dfb0a13c1fe27da,
title = "Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure",
abstract = "The main objective of the present study was to investigate the toxic effect of long-term exposure to DDT (2,2-dichlorodiphenyl-1,1,1-trichloroethane) on rat livers. Female Wistar rats were treated with once-weekly i.p. doses of DDT (10 and 50 mg/kg) for 12 weeks. Histological analysis revealed significant changes in the liver structure, especially at a dose of 50 mg/kg, which consistent with a fibrotic state. Long-term DDT exposure increased micro RNA-21 (miR-21) level and decreased Acetyl-CoA acetyltransferase 1 (Acat1) mRNA and protein levels in a dose-dependent manner. A dual-luciferase reporter assay confirmed the regulation of the rat Acat1 3′-UTR by miR-21. Previous studies have described the involvement of ACAT1 in fibrogenesis; thus, regulation of the Acat1 gene by miR-21 may play a role in DDT exposure-mediated liver fibrosis.",
keywords = "Acat1, DDT, liver fibrosis, mir-21, rat, TARGET, GENE, MICRORNAS, EXPRESSION, PESTICIDES, ACAT1",
author = "Chanyshev, {Mikhail D.} and Yarushkin, {Andrey A.} and Koldysheva, {Elena V.} and Lushnikova, {Elena L.} and Gulyaeva, {Lyudmila F.}",
year = "2020",
month = oct,
day = "12",
doi = "10.1080/15376516.2020.1777493",
language = "English",
volume = "30",
pages = "562--569",
journal = "Toxicology Mechanisms and Methods",
issn = "1537-6516",
publisher = "Informa Healthcare",
number = "8",

}

RIS

TY - JOUR

T1 - Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure

AU - Chanyshev, Mikhail D.

AU - Yarushkin, Andrey A.

AU - Koldysheva, Elena V.

AU - Lushnikova, Elena L.

AU - Gulyaeva, Lyudmila F.

PY - 2020/10/12

Y1 - 2020/10/12

N2 - The main objective of the present study was to investigate the toxic effect of long-term exposure to DDT (2,2-dichlorodiphenyl-1,1,1-trichloroethane) on rat livers. Female Wistar rats were treated with once-weekly i.p. doses of DDT (10 and 50 mg/kg) for 12 weeks. Histological analysis revealed significant changes in the liver structure, especially at a dose of 50 mg/kg, which consistent with a fibrotic state. Long-term DDT exposure increased micro RNA-21 (miR-21) level and decreased Acetyl-CoA acetyltransferase 1 (Acat1) mRNA and protein levels in a dose-dependent manner. A dual-luciferase reporter assay confirmed the regulation of the rat Acat1 3′-UTR by miR-21. Previous studies have described the involvement of ACAT1 in fibrogenesis; thus, regulation of the Acat1 gene by miR-21 may play a role in DDT exposure-mediated liver fibrosis.

AB - The main objective of the present study was to investigate the toxic effect of long-term exposure to DDT (2,2-dichlorodiphenyl-1,1,1-trichloroethane) on rat livers. Female Wistar rats were treated with once-weekly i.p. doses of DDT (10 and 50 mg/kg) for 12 weeks. Histological analysis revealed significant changes in the liver structure, especially at a dose of 50 mg/kg, which consistent with a fibrotic state. Long-term DDT exposure increased micro RNA-21 (miR-21) level and decreased Acetyl-CoA acetyltransferase 1 (Acat1) mRNA and protein levels in a dose-dependent manner. A dual-luciferase reporter assay confirmed the regulation of the rat Acat1 3′-UTR by miR-21. Previous studies have described the involvement of ACAT1 in fibrogenesis; thus, regulation of the Acat1 gene by miR-21 may play a role in DDT exposure-mediated liver fibrosis.

KW - Acat1

KW - DDT

KW - liver fibrosis

KW - mir-21

KW - rat

KW - TARGET

KW - GENE

KW - MICRORNAS

KW - EXPRESSION

KW - PESTICIDES

KW - ACAT1

UR - http://www.scopus.com/inward/record.url?scp=85087515047&partnerID=8YFLogxK

U2 - 10.1080/15376516.2020.1777493

DO - 10.1080/15376516.2020.1777493

M3 - Article

C2 - 32508177

AN - SCOPUS:85087515047

VL - 30

SP - 562

EP - 569

JO - Toxicology Mechanisms and Methods

JF - Toxicology Mechanisms and Methods

SN - 1537-6516

IS - 8

ER -

ID: 24722238