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DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila. / Posukh, Olga V.; Shloma, Victor V.; Skrypnik, Polina A. et al.

In: Cells, Vol. 14, No. 16, 1243, 12.08.2025.

Research output: Contribution to journal › Article › peer-review

Harvard

Posukh, OV, Shloma, VV, Skrypnik, PA, Maksimov, DA, Antoshina, PA, Kalashnikova, DA, Nurislamov, A, Lukyanchikova, VA, Torgunakov, N, Battulin, NR, Fishman, VS, Vyatkin, YV, Smelova, AA, Romanov, SE, Laktionov, PP, Valishayev, D, Belyakin, SN & Singh, PB 2025, 'DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila', Cells, vol. 14, no. 16, 1243. https://doi.org/10.3390/cells14161243

APA

Posukh, O. V., Shloma, V. V., Skrypnik, P. A., Maksimov, D. A., Antoshina, P. A., Kalashnikova, D. A., Nurislamov, A., Lukyanchikova, V. A., Torgunakov, N., Battulin, N. R., Fishman, V. S., Vyatkin, Y. V., Smelova, A. A., Romanov, S. E., Laktionov, P. P., Valishayev, D., Belyakin, S. N., & Singh, P. B. (2025). DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila. Cells, 14(16), [1243]. https://doi.org/10.3390/cells14161243

Vancouver

Posukh OV, Shloma VV, Skrypnik PA, Maksimov DA, Antoshina PA, Kalashnikova DA et al. DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila. Cells. 2025 Aug 12;14(16):1243. doi: 10.3390/cells14161243

Author

Posukh, Olga V. ; Shloma, Victor V. ; Skrypnik, Polina A. et al. / DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila. In: Cells. 2025 ; Vol. 14, No. 16.

BibTeX

@article{6d7b1bfe35ff4a4185c5bc48b27fa624,
title = "DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila",
abstract = "The term chromosomal imprinting was introduced to denote the parent-of-origin-dependent behavior of chromosomes in the fungus gnat originally named Sciara coprophila (current taxonomic name is Bradysia coprophila). Such behavior is observed in Sciara coprophila embryos, where paternal X chromosomes (Xp) are specifically eliminated during the 7th–8th cleavage divisions. Elimination is regulated by a controlling element (CE) that has been mapped to heterochromomere II (H2) within the sub-telomeric short arm of polytene X chromosomes. Here, using a combination of a new Sciara genome assembly, along with ChIP-Seq and MeDIP analyses, we show that a 1.2 Mb region within the CE locus has a repressive epigenetic signature that is characterised by enrichments of H3K9me3, H4K20me3 and 5′-methyl cytosine (5meC). These data provide evidence for a model where the H3K9me3/HP1/H4K20me3 pathway operates to assemble a heterochromatin-like complex at the CE that renders it silent on Xp chromosomes that are not eliminated. In this regard, our findings support the idea that the H3K9me3/HP1/H4K20me3 pathway represents the most evolutionarily conserved mechanism linked to chromosomal imprinting in animals.",
keywords = "DNA methylation, H3K9me3, H4K20me3, HP1, genomic imprinting, heterochromatin-like complexes",
author = "Posukh, {Olga V.} and Shloma, {Victor V.} and Skrypnik, {Polina A.} and Maksimov, {Daniil A.} and Antoshina, {Polina A.} and Kalashnikova, {Daria A.} and Artem Nurislamov and Lukyanchikova, {Varvara A.} and Nikita Torgunakov and Battulin, {Nariman R.} and Fishman, {Veniamin S.} and Vyatkin, {Yuri V.} and Smelova, {Arina A.} and Romanov, {Stanislav E.} and Laktionov, {Petr P.} and Daulet Valishayev and Belyakin, {Stepan N.} and Singh, {Prim B.}",
note = "This work was funded by the Nazarbayev University Faculty Development Grant 201223FD8818 awarded to PBS. Funds were also provided by a grant from the Ministry of Education and Science of the Russian Federation #14.Y26.31.0024.",
year = "2025",
month = aug,
day = "12",
doi = "10.3390/cells14161243",
language = "English",
volume = "14",
journal = "Cells",
issn = "2073-4409",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "16",

}

RIS

TY - JOUR

T1 - DNA and Histone Modifications Identify a Putative Controlling Element (CE) on the X Chromosome of Sciara coprophila

AU - Posukh, Olga V.

AU - Shloma, Victor V.

AU - Skrypnik, Polina A.

AU - Maksimov, Daniil A.

AU - Antoshina, Polina A.

AU - Kalashnikova, Daria A.

AU - Nurislamov, Artem

AU - Lukyanchikova, Varvara A.

AU - Torgunakov, Nikita

AU - Battulin, Nariman R.

AU - Fishman, Veniamin S.

AU - Vyatkin, Yuri V.

AU - Smelova, Arina A.

AU - Romanov, Stanislav E.

AU - Laktionov, Petr P.

AU - Valishayev, Daulet

AU - Belyakin, Stepan N.

AU - Singh, Prim B.

N1 - This work was funded by the Nazarbayev University Faculty Development Grant 201223FD8818 awarded to PBS. Funds were also provided by a grant from the Ministry of Education and Science of the Russian Federation #14.Y26.31.0024.

PY - 2025/8/12

Y1 - 2025/8/12

N2 - The term chromosomal imprinting was introduced to denote the parent-of-origin-dependent behavior of chromosomes in the fungus gnat originally named Sciara coprophila (current taxonomic name is Bradysia coprophila). Such behavior is observed in Sciara coprophila embryos, where paternal X chromosomes (Xp) are specifically eliminated during the 7th–8th cleavage divisions. Elimination is regulated by a controlling element (CE) that has been mapped to heterochromomere II (H2) within the sub-telomeric short arm of polytene X chromosomes. Here, using a combination of a new Sciara genome assembly, along with ChIP-Seq and MeDIP analyses, we show that a 1.2 Mb region within the CE locus has a repressive epigenetic signature that is characterised by enrichments of H3K9me3, H4K20me3 and 5′-methyl cytosine (5meC). These data provide evidence for a model where the H3K9me3/HP1/H4K20me3 pathway operates to assemble a heterochromatin-like complex at the CE that renders it silent on Xp chromosomes that are not eliminated. In this regard, our findings support the idea that the H3K9me3/HP1/H4K20me3 pathway represents the most evolutionarily conserved mechanism linked to chromosomal imprinting in animals.

AB - The term chromosomal imprinting was introduced to denote the parent-of-origin-dependent behavior of chromosomes in the fungus gnat originally named Sciara coprophila (current taxonomic name is Bradysia coprophila). Such behavior is observed in Sciara coprophila embryos, where paternal X chromosomes (Xp) are specifically eliminated during the 7th–8th cleavage divisions. Elimination is regulated by a controlling element (CE) that has been mapped to heterochromomere II (H2) within the sub-telomeric short arm of polytene X chromosomes. Here, using a combination of a new Sciara genome assembly, along with ChIP-Seq and MeDIP analyses, we show that a 1.2 Mb region within the CE locus has a repressive epigenetic signature that is characterised by enrichments of H3K9me3, H4K20me3 and 5′-methyl cytosine (5meC). These data provide evidence for a model where the H3K9me3/HP1/H4K20me3 pathway operates to assemble a heterochromatin-like complex at the CE that renders it silent on Xp chromosomes that are not eliminated. In this regard, our findings support the idea that the H3K9me3/HP1/H4K20me3 pathway represents the most evolutionarily conserved mechanism linked to chromosomal imprinting in animals.

KW - DNA methylation

KW - H3K9me3

KW - H4K20me3

KW - HP1

KW - genomic imprinting

KW - heterochromatin-like complexes

UR - https://www.scopus.com/pages/publications/105014343743

UR - https://www.mendeley.com/catalogue/3191fe0b-f7d3-3144-b6cb-ebb2786ee2e8/

U2 - 10.3390/cells14161243

DO - 10.3390/cells14161243

M3 - Article

C2 - 40862722

VL - 14

JO - Cells

JF - Cells

SN - 2073-4409

IS - 16

M1 - 1243

ER -

ID: 68972378