Research output: Contribution to journal › Article › peer-review
Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes. / Guselnikov, Sergey V.; Baranov, Konstantin O.; Najakshin, Alexander M. et al.
In: Frontiers in Immunology, Vol. 9, No. MAY, 1079, 28.05.2018, p. 1079.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes
AU - Guselnikov, Sergey V.
AU - Baranov, Konstantin O.
AU - Najakshin, Alexander M.
AU - Mechetina, Ludmila V.
AU - Chikaev, Nikolai A.
AU - Makunin, Alexey I.
AU - Kulemzin, Sergey V.
AU - Andreyushkova, Daria A.
AU - Stöck, Matthias
AU - Wuertz, Sven
AU - Gessner, Jörn
AU - Warren, Wesley C.
AU - Schartl, Manfred
AU - Trifonov, Vladimir A.
AU - Taranin, Alexander V.
PY - 2018/5/28
Y1 - 2018/5/28
N2 - The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish. First, based on the bioinformatic analysis of recent transcriptomic and genomic resources, we experimentally characterized the IgL genes in the chondrostean fish, Acipenser ruthenus (sterlet). We show that this species has three loci encoding IgL kappa-like chains with a translocon-type gene organization and a single VJC cluster, encoding homogeneous lambda-like light chain. In addition, sterlet possesses sigma-like VL and J-CL genes, which are transcribed separately and both encode protein products with cleavable leader peptides. The Acipenseriformes IgL dataset was extended by the sequences mined in the databases of species belonging to other non-teleost lineages of ray-finned fish: Holostei and Polypteriformes. Inclusion of these new data into phylogenetic analysis showed a clear subdivision of IgL chains into five groups. The isotype described previously as the teleostean IgL lambda turned out to be a kappa and lambda chain paralog that emerged before the radiation of ray-finned fish. We designate this isotype as lambda-2. The phylogeny also showed that sigma-2 IgL chains initially regarded as specific for cartilaginous fish are present in holosteans, polypterids, and even in turtles. We conclude that there were five ancient IgL isotypes, which evolved differentially in various lineages of jawed vertebrates.
AB - The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish. First, based on the bioinformatic analysis of recent transcriptomic and genomic resources, we experimentally characterized the IgL genes in the chondrostean fish, Acipenser ruthenus (sterlet). We show that this species has three loci encoding IgL kappa-like chains with a translocon-type gene organization and a single VJC cluster, encoding homogeneous lambda-like light chain. In addition, sterlet possesses sigma-like VL and J-CL genes, which are transcribed separately and both encode protein products with cleavable leader peptides. The Acipenseriformes IgL dataset was extended by the sequences mined in the databases of species belonging to other non-teleost lineages of ray-finned fish: Holostei and Polypteriformes. Inclusion of these new data into phylogenetic analysis showed a clear subdivision of IgL chains into five groups. The isotype described previously as the teleostean IgL lambda turned out to be a kappa and lambda chain paralog that emerged before the radiation of ray-finned fish. We designate this isotype as lambda-2. The phylogeny also showed that sigma-2 IgL chains initially regarded as specific for cartilaginous fish are present in holosteans, polypterids, and even in turtles. We conclude that there were five ancient IgL isotypes, which evolved differentially in various lineages of jawed vertebrates.
KW - Acipenser ruthenus
KW - Acipenseriformes
KW - Evolution
KW - Holostei
KW - Immunoglobulin light chain
KW - Polypteriformes
KW - B-CELL DEVELOPMENT
KW - RAINBOW-TROUT
KW - immunoglobulin light chain
KW - GENOMIC ORGANIZATION
KW - STERLET ACIPENSER-RUTHENUS
KW - evolution
KW - HEAVY-CHAIN
KW - IMMUNE-SYSTEM
KW - MESSENGER-RNA
KW - JAWED VERTEBRATES
KW - CDNA SEQUENCE
KW - SIBERIAN STURGEON
UR - http://www.scopus.com/inward/record.url?scp=85047509373&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2018.01079
DO - 10.3389/fimmu.2018.01079
M3 - Article
C2 - 29892283
AN - SCOPUS:85047509373
VL - 9
SP - 1079
JO - Frontiers in Immunology
JF - Frontiers in Immunology
SN - 1664-3224
IS - MAY
M1 - 1079
ER -
ID: 13633111