Research output: Contribution to journal › Article › peer-review
Diversity of Archaea in Bottom Sediments of the Discharge Areas With Oil- and Gas-Bearing Fluids in Lake Baikal. / Lomakina, Anna V.; Mamaeva, Elena V.; Galachyants, Yuri P. et al.
In: Geomicrobiology Journal, Vol. 35, No. 1, 02.01.2018, p. 50-63.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Diversity of Archaea in Bottom Sediments of the Discharge Areas With Oil- and Gas-Bearing Fluids in Lake Baikal
AU - Lomakina, Anna V.
AU - Mamaeva, Elena V.
AU - Galachyants, Yuri P.
AU - Petrova, Darya P.
AU - Pogodaeva, Tatyana V.
AU - Shubenkova, Olga V.
AU - Khabuev, Andrey V.
AU - Morozov, Igor V.
AU - Zemskaya, Tamara I.
N1 - Publisher Copyright: © 2017 Taylor & Francis Group, LLC.
PY - 2018/1/2
Y1 - 2018/1/2
N2 - Using massively parallel sequencing (the Roche 454 platform) we have studied the diversity of archaeal 16S rRNA gene sequences in oxic and anoxic sediments at six sites in Lake Baikal with oil- and gas-bearing fluids discharge. Archaeal communities appeared to be represented mainly by five phyla: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Bathyarchaeota (miscellaneous Crenarchaeotic group), and Woesearchaeota (deep sea hydrothermal vent group 6). Among them we detected sequences of methanogens of the orders Methanomicrobiales, Methanosarsinales, Methanococcales, as well as representatives of the following uncultured archaeal lineages: Group C3, Marine Benthic Group D, and Terrestrial Miscellaneous Group. We have also identified sequences of ammonia-oxidizing archaea of the phyla Crenarchaeota and Thaumarchaeota. Phylogenetic analysis showed the presence ANME-2d-related sequences. However, the analysis of mcrA genes libraries has not revealed typical representatives of ANME groups. Comparison of amplicon libraries 16S rRNA gene fragments from different samples proved the widespread presence of previously detected Baikal archaeal lineages, which are members of the phylum Crenarchaeota and Thaumarchaeota (formerly Group C3 of Crenarchaeota).
AB - Using massively parallel sequencing (the Roche 454 platform) we have studied the diversity of archaeal 16S rRNA gene sequences in oxic and anoxic sediments at six sites in Lake Baikal with oil- and gas-bearing fluids discharge. Archaeal communities appeared to be represented mainly by five phyla: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Bathyarchaeota (miscellaneous Crenarchaeotic group), and Woesearchaeota (deep sea hydrothermal vent group 6). Among them we detected sequences of methanogens of the orders Methanomicrobiales, Methanosarsinales, Methanococcales, as well as representatives of the following uncultured archaeal lineages: Group C3, Marine Benthic Group D, and Terrestrial Miscellaneous Group. We have also identified sequences of ammonia-oxidizing archaea of the phyla Crenarchaeota and Thaumarchaeota. Phylogenetic analysis showed the presence ANME-2d-related sequences. However, the analysis of mcrA genes libraries has not revealed typical representatives of ANME groups. Comparison of amplicon libraries 16S rRNA gene fragments from different samples proved the widespread presence of previously detected Baikal archaeal lineages, which are members of the phylum Crenarchaeota and Thaumarchaeota (formerly Group C3 of Crenarchaeota).
KW - Archaeal communities
KW - Baikal archaeal lineages and ANME-2d
KW - Lake Baikal
KW - oil and methane seeps
KW - pyrosequencing
UR - http://www.scopus.com/inward/record.url?scp=85019252057&partnerID=8YFLogxK
U2 - 10.1080/01490451.2017.1315195
DO - 10.1080/01490451.2017.1315195
M3 - Article
AN - SCOPUS:85019252057
VL - 35
SP - 50
EP - 63
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
SN - 0149-0451
IS - 1
ER -
ID: 9444799