Research output: Contribution to journal › Article › peer-review
Differential alternative splicing in brain regions of rats selected for aggressive behavior. / Babenko, V. N.; Bragin, A. O.; Chadaeva, I. V. et al.
In: Molekuliarnaia biologiia, Vol. 51, No. 5, 09.11.2017, p. 870-880.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Differential alternative splicing in brain regions of rats selected for aggressive behavior
AU - Babenko, V. N.
AU - Bragin, A. O.
AU - Chadaeva, I. V.
AU - Markel, A. L.
AU - Orlov, Y. L.
PY - 2017/11/9
Y1 - 2017/11/9
N2 - Profiles of alternative mRNA isoforms have been determined in three brain regions of rats from an aggressive and a tame line selected for 74 generations. Among 2319 genes with alternatively spliced exons, approximately 84% were confirmed by analyzing public databases. Based on Gene Ontology-guided clustering of alternatively spliced genes, it has been found that the sample was enriched in synapse-specific genes (FDR < 10^(-17)). Patterns of gene expression in the brains of animals with genetically determined high or low aggression were more frequently found to differ in the use of alternatively spliced exons than in animals environmentally conditioned for increased or lowered propensity to aggression. For the Adcyap1r1 gene, five alternatively spliced mRNA isoforms have been represented differentially in aggressive animals. A detailed analysis of the gene that encodes glutamate ionotropic receptor NMDA type subunit 1 (Grin1) has confirmed significant differences in the levels of its alternatively spliced isoforms in certain brain regions of tame and aggressive rats. These differences may affect the behavior in rats genetically selected for aggression levels.
AB - Profiles of alternative mRNA isoforms have been determined in three brain regions of rats from an aggressive and a tame line selected for 74 generations. Among 2319 genes with alternatively spliced exons, approximately 84% were confirmed by analyzing public databases. Based on Gene Ontology-guided clustering of alternatively spliced genes, it has been found that the sample was enriched in synapse-specific genes (FDR < 10^(-17)). Patterns of gene expression in the brains of animals with genetically determined high or low aggression were more frequently found to differ in the use of alternatively spliced exons than in animals environmentally conditioned for increased or lowered propensity to aggression. For the Adcyap1r1 gene, five alternatively spliced mRNA isoforms have been represented differentially in aggressive animals. A detailed analysis of the gene that encodes glutamate ionotropic receptor NMDA type subunit 1 (Grin1) has confirmed significant differences in the levels of its alternatively spliced isoforms in certain brain regions of tame and aggressive rats. These differences may affect the behavior in rats genetically selected for aggression levels.
KW - aggressive behavior
KW - alternative splicing
KW - Grin1
KW - RNA profiling
KW - synaptic conduction genes
KW - Aggression
KW - Alternative Splicing
KW - Animals
KW - Brain/metabolism
KW - Male
KW - RNA, Messenger/genetics
KW - Rats
KW - Receptors, N-Methyl-D-Aspartate/biosynthesis
KW - Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I/biosynthesis
KW - Selective Breeding
UR - http://www.scopus.com/inward/record.url?scp=85049065044&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=29959244
U2 - 10.7868/S0026898417050159
DO - 10.7868/S0026898417050159
M3 - Article
C2 - 29116075
AN - SCOPUS:85049065044
VL - 51
SP - 870
EP - 880
JO - Molekulyarnaya Biologiya
JF - Molekulyarnaya Biologiya
SN - 0026-8984
IS - 5
ER -
ID: 14279790